CD49a antibody | TS2/7
FITC conjugated Mouse anti Human CD49a antibody, clone TS2/7 (MCA1133F) used to evaluate VLA-1 expression on human dermal cells by flow cytometry.
Image caption:
Flow cytometric analysis to detect the incidence of dermal pericytes in neonatal versus aged skin.
A, B. Dual staining for CD45 and CD49a (VLA-1) and analysis of the CD45- fraction of dermal cells illustrates a higher incidence of CD49abri skin pericytes in neonatal foreskin (A, n=6) than aged 48-59yo adult skin (B, n=6). C. Quantitation of pooled data from A, B showing lower incidence of pericytes in aged skin versus neonatal skin (***=p<001). Error bars represent mean ± SD.
From: Zhuang, L.; Visalakshan, R.M.; Kaur, P.
Dermal Pericytes Exhibit Declined Ability to Promote Human Skin Regeneration with Ageing in 3D Organotypic Culture Models.
Cells 2021, 10, 3051.
doi: 10.3390/cells10113051
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD49a:Alexa Fluor® 647
Mouse anti Human CD49a:FITC
Mouse anti Human CD49a
Mouse anti Human CD49a:RPE
- Product Type
- Monoclonal Antibody
- Clone
- TS2/7
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA1133A647 | F | 100 Tests/1ml |
|
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| MCA1133A647T | F | 25 Tests/0.25ml |
|
|
| MCA1133F | F | 0.1 mg |
|
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| MCA1133G | C F IP | 0.2 mg |
|
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| MCA1133GT | C F IP | 25 µg |
|
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| MCA1133PE | F | 100 Tests |
|
Mouse anti Human CD49a antibody, clone TS2/7 recognizes the human alpha 1 integrin sub-unit, which forms the VLA-1 heterodimer in association with the beta 1 integrin. VLA-1 is a receptor for collagen and laminin, and is expressed by chronically activated T cells, melanoma cells and smooth muscle cells.
Mouse anti Human CD49a antibody, clone TS2/7 has been reported as being suitable for use in Western Blotting.
Mouse anti Human CD49a antibody, clone TS2/7 is routinely tested in flow cytometry using HeLa cells.
Mouse anti Human CD49a antibody, clone TS2/7 has been reported as being suitable for use in Western Blotting.
Mouse anti Human CD49a antibody, clone TS2/7 is routinely tested in flow cytometry using HeLa cells.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Human CTL line directed against HLA-DR.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb.c mice were fused with cells of the NS1 mouse myeloma.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P56199
- Entrez Gene
- ITGA1
- GO Terms
- GO:0006936 muscle contraction
- GO:0004872 receptor activity
- GO:0005518 collagen binding
- GO:0008305 integrin complex
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007411 axon guidance
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD49a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | 1/25 | 1/50 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
|
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| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD49a antibody
-
Hemler, M.E. et al. (1984) Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines.
J Immunol. 132 (6): 3011-8. -
Hemler, M.E. et al. (1985) Biochemical characterization of VLA-1 and VLA-2. Cell surface heterodimers on activated T cells.
J Biol Chem. 260 (28): 15246-52. -
Hafler, D.A. et al. (1985) Investigation of in vivo activated T cells in multiple sclerosis and inflammatory central nervous system diseases.
Clin Immunol Immunopathol. 37 (2): 163-71. -
Klemmt, P.A. et al. (2007) Endometrial cells from women with endometriosis have increased adhesion and proliferative capacity in response to extracellular matrix components: towards a mechanistic model for endometriosis progression.
Hum Reprod. 22: 3139-47. -
Berry, P.A. et al. (2011) The calcium sensor STIM1 is regulated by androgens in prostate stromal cells.
Prostate. 71: 1646-55. -
Elshaw, S.R. et al. (2001) A comparison of ocular melanocyte and uveal melanoma cell invasion and the implication of alpha1beta1, alpha4beta1 and alpha6beta1 integrins.
Br J Ophthalmol. 85: 732-8. -
Cervar-Zivkovic, M. et al. (2011) Endothelin-1 Stimulates Proliferation of First-Trimester Trophoblasts via the A- and B-Type Receptor and Invasion via the B-Type Receptor.
J Clin Endocrinol Metab. 96: 3408-15. -
Kaneko, Y. et al. (2012) Integrin α1/β1 and α2/β1 as a receptor for IgA1 in human glomerular mesangial cells in IgA nephropathy.
Int Immunol. 24: 219-32. -
Majali-Martinez, A. et al. (2018) Temporal changes of the endothelin system in human cytotrophoblasts during the first trimester of pregnancy.
Physiol Res. 67 (Suppl 1): S247-S255. -
Zhuang, L. et al. (2021) Dermal Pericytes Exhibit Declined Ability to Promote Human Skin Regeneration with Ageing in 3D Organotypic Culture Models
Cells. 10 (11): 3051.
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