CD49a antibody | TS2/7
FITC conjugated Mouse anti Human CD49a antibody, clone TS2/7 (MCA1133F) used to evaluate VLA-1 expression on human dermal cells by flow cytometry.
Image caption:
Flow cytometric analysis to detect the incidence of dermal pericytes in neonatal versus aged skin.
A, B. Dual staining for CD45 and CD49a (VLA-1) and analysis of the CD45- fraction of dermal cells illustrates a higher incidence of CD49abri skin pericytes in neonatal foreskin (A, n=6) than aged 48-59yo adult skin (B, n=6). C. Quantitation of pooled data from A, B showing lower incidence of pericytes in aged skin versus neonatal skin (***=p<001). Error bars represent mean ± SD.
From: Zhuang, L.; Visalakshan, R.M.; Kaur, P.
Dermal Pericytes Exhibit Declined Ability to Promote Human Skin Regeneration with Ageing in 3D Organotypic Culture Models.
Cells 2021, 10, 3051.
doi: 10.3390/cells10113051
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD49a
- Product Type
- Monoclonal Antibody
- Clone
- TS2/7
- Isotype
- IgG1
- Specificity
- CD49a
Antibody Quality and Performance Guarantee
| Mouse anti Human CD49a antibody, clone TS2/7 recognizes the human alpha 1 integrin sub-unit, which forms the VLA-1 heterodimer in association with the beta 1 integrin. VLA-1 is a receptor for collagen and laminin, and is expressed by chronically activated T cells, melanoma cells and smooth muscle cells. Mouse anti Human CD49a antibody, clone TS2/7 has been reported as being suitable for use in Western Blotting. Mouse anti Human CD49a antibody, clone TS2/7 is routinely tested in flow cytometry using HeLa cells. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human CTL line directed against HLA-DR.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb.c mice were fused with cells of the NS1 mouse myeloma.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/50 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Hemler, M.E. et al. (1984) Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines.
J Immunol. 132 (6): 3011-8.
References for CD49a antibody
-
Hemler, M.E. et al. (1985) Biochemical characterization of VLA-1 and VLA-2. Cell surface heterodimers on activated T cells.
J Biol Chem. 260 (28): 15246-52. -
Hafler, D.A. et al. (1985) Investigation of in vivo activated T cells in multiple sclerosis and inflammatory central nervous system diseases.
Clin Immunol Immunopathol. 37 (2): 163-71. -
Klemmt, P.A. et al. (2007) Endometrial cells from women with endometriosis have increased adhesion and proliferative capacity in response to extracellular matrix components: towards a mechanistic model for endometriosis progression.
Hum Reprod. 22: 3139-47. -
Berry, P.A. et al. (2011) The calcium sensor STIM1 is regulated by androgens in prostate stromal cells.
Prostate. 71: 1646-55. -
Elshaw, S.R. et al. (2001) A comparison of ocular melanocyte and uveal melanoma cell invasion and the implication of alpha1beta1, alpha4beta1 and alpha6beta1 integrins.
Br J Ophthalmol. 85: 732-8. -
Cervar-Zivkovic, M. et al. (2011) Endothelin-1 Stimulates Proliferation of First-Trimester Trophoblasts via the A- and B-Type Receptor and Invasion via the B-Type Receptor.
J Clin Endocrinol Metab. 96: 3408-15. -
Kaneko, Y. et al. (2012) Integrin α1/β1 and α2/β1 as a receptor for IgA1 in human glomerular mesangial cells in IgA nephropathy.
Int Immunol. 24: 219-32. -
Majali-Martinez, A. et al. (2018) Temporal changes of the endothelin system in human cytotrophoblasts during the first trimester of pregnancy.
Physiol Res. 67 (Suppl 1): S247-S255.
View The Latest Product References
-
Zhuang, L. et al. (2021) Dermal Pericytes Exhibit Declined Ability to Promote Human Skin Regeneration with Ageing in 3D Organotypic Culture Models
Cells. 10 (11): 3051.
- Synonyms
- Integrin Alpha 1 Chain
- VLA-1
- RRID
- AB_566785
- UniProt
- P56199
- Entrez Gene
- ITGA1
- GO Terms
- GO:0006936 muscle contraction
- GO:0004872 receptor activity
- GO:0005518 collagen binding
- GO:0008305 integrin complex
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007411 axon guidance
MCA1133G
155087
1702
MCA1133GT
154565
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