CD46 antibody | rA44B44-08/4D7
Mouse anti Human CD46
- Product Type
- Monoclonal Antibody
- Clone
- rA44B44-08/4D7
- Isotype
- IgG1
- Specificity
- CD46
Antibody Quality and Performance Guarantee
TZA0165GA
Recombinant Antibodies
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| Mouse anti Human CD46, clone rA44B44-08/4D7, is a recombinant monoclonal antibody that recognizes CD46, also known as membrane cofactor protein (MCP), Trophoblast leukocyte common antigen or TLX. CD46 is a single-pass type I transmembrane protein expressed by almost all nucleated cells. Four major isoforms exist, each of which has an amino terminus containing four complement control protein modules and an alternatively spliced O-glycosylated domain. The cytoplasmic region consists of an anchor region and an alternatively spliced cytoplasmic tail containing a distinct signaling motif. CD46 has a role in regulating complement activation by binding C3b and C4b after deposition on a host cell, facilitating cleavage. The CD46 C2 isoform is exclusively expressed on the inner acrosomal membrane of human spermatozoa, where is serves a role in fertilization. CD46 is also involved in T cell activation, autophagy and the intracellular complement system. CD46 is often dysregulated in cancer, for example in multiple myeloma and various solid tumors. It has been reported as a potential tumor target and prognostic indicator. CD46 is a receptor or adherence factor for a number of pathogens, including the measles virus. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant CD46 Cys35-Asp343 expressed by mammalian cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
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