CD45RO antibody | UCHL1

Mouse anti Human CD45RO

Product Type
Monoclonal Antibody
Clone
UCHL1
Isotype
IgG2a
Specificity
CD45RO

Product Code Applications Pack Size List Price Your Price Qty
MCA461GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P 0.1 mg loader
List Price Your Price
loader
MCA461GT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P 25 µg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD45RO monoclonal antibody, clone UCHL1 recognizes the low molecular weight isoform (180 kDa) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype.

In peripheral blood Mouse anti Human CD45RO, clone UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
Marmoset
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA461GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA461GT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human IL-2 dependent T cells
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Tonsil

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References for CD45RO antibody

  1. Smith, S.H. et al. (1986) Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.
    Immunology 58: 63-70.
  2. Norton, A.J. et al. (1986) Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues.
    J Clin Pathol. 39 (4): 399-405.
  3. Beverley, P.C.L. et al. (1986) T-cell subsets and function. Progress in Immunology VI. Cinader, B., Miller, G.G., eds.
    Academic Press Orlando pp 941-948.
  4. Beverley, P.C. (1987) Human T cell subsets.
    Immunol Lett. 14 (4): 263-7.
  5. Terry. L.A. et al. (1987) Phenotypic heterogeneity of the CD4+ and CD8+ subsets.
    Leucocyte Typing III. McMichael, A.J., Beverley, P.C.L. et al. eds. University Press. pp 225-7.
  6. Akbar, A.N. et al. (1988) Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells.
    J Immunol. 140 (7): 2171-8.
  7. Terry, L.A. et al. (1988) The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45.
    Immunology. 64 (2): 331-6.
  8. Beverley, P.C.L. et al. (1988) Phenotypic diversity of the CD45 antigen and its relation to function.
    Immunology, Suppl. 1: 3-5.
  9. View The Latest Product References
  10. Merkenschlager, M. et al. (1988) Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation.
    Eur J Immunol. 18 (11): 1653-61.
  11. Cavers, M. et al. (2002) Differential expression of beta1 and beta2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations.
    Clin Exp Immunol. 127: 60-5.
  12. Hutnick, N.A. et al. (2010) Vaccination with Ad5 vectors expands Ad5-specific CD8 T cells without altering memory phenotype or functionality.
    PLoS One. 5: e14385.
  13. Leigh, J.E. et al. (2006) Characterization of the immune status of CD8+ T cells in oral lesions of human immunodeficiency virus-infected persons with oropharyngeal Candidiasis.
    Clin Vaccine Immunol. 13: 678-83.
  14. Nistala, K. et al. (2008) Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
    Arthritis Rheum. 58: 875-87.
  15. Liu Y et al. (2015) Fractionation of human spermatogenic cells using STA-PUT gravity sedimentation and their miRNA profiling.
    Sci Rep. 5: 8084.
  16. Wilson, C.L. et al. (2015) Ubiquitin C-terminal hydrolase 1: A novel functional marker for liver myofibroblasts and a therapeutic target in chronic liver disease.
    J Hepatol. 63 (6): 1421-8.

Flow Cytometry

Immunohistology - Frozen

RRID
AB_324651
UniProt
P08575
Entrez Gene
PTPRC
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002378 immunoglobulin biosynthetic process
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007411 axon guidance
GO:0019901 protein kinase binding
GO:0030890 positive regulation of B cell proliferation
GO:0033261 regulation of S phase
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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