CD45 antibody | HI30

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Mouse anti Human CD45:FITC

Mouse anti Human CD45

Mouse anti Human CD45:RPE

Mouse anti Human CD45:RPE-Cy5

Product Type
Monoclonal Antibody
Clone
HI30
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1719F F 0.1 mg
MCA1719GA F P 0.1 mg
MCA1719PE F 100 Tests/0.5ml
MCA1719C F 100 Tests
Mouse anti Human CD45 antibody, clone HI30 recognizes the human CD45 cell surface antigen, also known as the leukocyte common antigen.

CD45 is expressed by all leukocytes; expression on granulocytes being weaker than on lymphocytes.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Product Form
Purified IgG conjugated to RPE - Cy5 - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P08575 Related reagents
Entrez Gene
PTPRC Related reagents
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002378 immunoglobulin biosynthetic process
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007411 axon guidance
GO:0019901 protein kinase binding
GO:0030890 positive regulation of B cell proliferation
GO:0033261 regulation of S phase
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
Acknowledgements
Cy® and CyDye® are registered trademarks of GE Healthcare
Regulatory
For research purposes only

Applications of CD45 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5
Flow Cytometry Neat 1/10
Immunohistology - Paraffin 0.1 10ug/ml
Flow Cytometry Neat
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells or 100ul whole blood.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD45 antibody

  1. Alvarez-gonzalez, C. et al. (2013) Cord blood Lin(-)CD45(-) embryonic-like stem cells are a heterogeneous population that lack self-renewal capacity.
    PLoS One. 8 (6): e67968.
  2. Eksteen, B. et al. (2009) Gut homing receptors on CD8 T cells are retinoic acid dependent and not maintained by liver dendritic or stellate cells.
    Gastroenterology. 137 (1): 320-9.