CD44v6 antibody | VFF-7

Mouse anti Human CD44v6

Product Type
Monoclonal Antibody
Clone
VFF-7
Isotype
IgG1
Specificity
CD44v6

Product Code Applications Pack Size List Price Your Price Qty
MCA1730
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * WB 0.1 mg loader
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loader
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Mouse anti Human CD44v6 antibody, clone VFF-7 recognizes an epitope encoded by exon v6 on the variant portion of human CD44.

Target Species
Human
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c micewere fused with cells of the P3X63Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen 1/400 1/500
Immunohistology - Paraffin 1 1/200
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Mouse IgG1 Negative Control

References for CD44v6 antibody

  1. Neumayer, R. et al. (1999) CD44 expression in benign and malignant colorectal polyps.
    Dis Colon Rectum. 42 (1): 50-5.
  2. Koopman, G. et al. (1993) Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.
    J Exp Med. 177 (4): 897-904.
  3. Yoo, S.H. et al. (2005) Expression patterns of markers for type II pneumocytes in pulmonary sclerosing hemangiomas and fetal lung tissues.
    Arch Pathol Lab Med. 129 (7): 915-9.
  4. Kim, J.S. and Werth, V.P. (2011) Identification of specific chondroitin sulfate species in cutaneous autoimmune disease.
    J Histochem Cytochem. 59: 780-90.
  5. Chaiyarit, P. et al. (2008) Alteration of the expression of CD44 isoforms in oral epithelia and saliva from patients with oral lichen planus.
    J Clin Immunol. 28: 26-34.
  6. Shirure, V.S. et al. (2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.
    Am J Physiol Cell Physiol. 308 (1): C68-78.
  7. Noori, M.S. et al. (2018) An adhesion based approach for the detection of esophageal cancer.
    Integr Biol (Camb). 10 (12): 747-57.

RRID
AB_322693
UniProt
P16070
Entrez Gene
CD44
View more products with CD44 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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