CD44v6 antibody | VFF-7
Mouse anti Human CD44v6
- Product Type
- Monoclonal Antibody
- Clone
- VFF-7
- Isotype
- IgG1
- Specificity
- CD44v6
Antibody Quality and Performance Guarantee
| Mouse anti Human CD44v6 antibody, clone VFF-7 recognizes an epitope encoded by exon v6 on the variant portion of human CD44. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Immunogen
- Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c micewere fused with cells of the P3X63Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
| Immunohistology - Frozen | |
1/400 | 1/500 |
| Immunohistology - Paraffin 1 | |
1/200 | |
| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Koopman, G. et al. (1993) Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44.
J Exp Med. 177 (4): 897-904.
References for CD44v6 antibody
-
Neumayer, R. et al. (1999) CD44 expression in benign and malignant colorectal polyps.
Dis Colon Rectum. 42 (1): 50-5. -
Yoo, S.H. et al. (2005) Expression patterns of markers for type II pneumocytes in pulmonary sclerosing hemangiomas and fetal lung tissues.
Arch Pathol Lab Med. 129 (7): 915-9. -
Chaiyarit, P. et al. (2008) Alteration of the expression of CD44 isoforms in oral epithelia and saliva from patients with oral lichen planus.
J Clin Immunol. 28: 26-34. -
Kim, J.S. and Werth, V.P. (2011) Identification of specific chondroitin sulfate species in cutaneous autoimmune disease.
J Histochem Cytochem. 59: 780-90. -
Shirure, V.S. et al. (2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.
Am J Physiol Cell Physiol. 308 (1): C68-78. -
Noori, M.S. et al. (2018) An adhesion based approach for the detection of esophageal cancer.
Integr Biol (Camb). 10 (12): 747-57.
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