CD44v3 antibody | VFF-327

Mouse anti Human CD44v3

Product Type
Monoclonal Antibody
Clone
VFF-327
Isotype
IgG1
Specificity
CD44v3

Product Code Applications Pack Size List Price Your Price Qty
MCA1734
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * 0.1 mg loader
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loader
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Mouse anti Human CD44v3 antibody, clone VFF-327 recognizes an epitope encoded by exon V3 on the variant portion of human CD44.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 10ug/ml
Immunohistology - Frozen 10ug/ml
Immunohistology - Paraffin 1 10ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
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Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for CD44v3 antibody

  1. Alam, T.N. et al. (2004) Differential expression of CD44 during human prostate epithelial cell differentiation.
    J Histochem Cytochem. 52 (8): 1083-90.
  2. Kim, J.S. & Werth, V.P. (2011) Identification of specific chondroitin sulfate species in cutaneous autoimmune disease.
    J Histochem Cytochem. 59 (8): 780-90.
  3. Bauer, S. et al. (2006) Fibroblast activation protein is expressed by rheumatoid myofibroblast-like synoviocytes.
    Arthritis Res Ther. 8: R171.
  4. Hanley, W.D. et al. (2006) Variant isoforms of CD44 are P- and L-selectin ligands on colon carcinoma cells.
    FASEB J. 20: 337-9.
  5. Shirure, V.S. et al. (2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.
    Am J Physiol Cell Physiol. 308 (1): C68-78.
  6. Spadea, A. et al. (2019) Evaluating the Efficiency of Hyaluronic Acid for Tumor Targeting via CD44.
    Mol Pharm. 16 (6): 2481-93.
  7. Birzele, F. et al. (2015) Markers for the responsiveness to anti-cd44 antibodies
    Patent Application Publication: US 2015/0044232 A1
  8. Noori, M.S. et al. (2018) An adhesion based approach for the detection of esophageal cancer.
    Integr Biol (Camb). 10 (12): 747-57.
  9. View The Latest Product References

Immunohistology - Frozen

RRID
AB_322700
UniProt
P16070
Entrez Gene
CD44
View more products with CD44 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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