CD44v3 antibody | VFF-327
Mouse anti Human CD44v3 antibody, clone VFF-327 recognises an epitope encoded by exon V3 on the variant portion of human CD44.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- P16070 Related reagents
- Entrez Gene
- CD44 Related reagents
- For research purposes only
Applications of CD44v3 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||10ug/ml|
|Immunohistology - Paraffin 1||10ug/ml|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Mouse IgG1 Negative Control||MCA928||100 Tests||F|
Product Specific References
References for CD44v3 antibody
Alam, T.N. et al. (2004) Differential expression of CD44 during human prostate epithelial cell differentiation.
J Histochem Cytochem. 52 (8): 1083-90.
Kim, J.S. & Werth, V.P. (2011) Identification of specific chondroitin sulfate species in cutaneous autoimmune disease.
J Histochem Cytochem. 59 (8): 780-90.
Bauer, S. et al. (2006) Fibroblast activation protein is expressed by rheumatoid myofibroblast-like synoviocytes.
Arthritis Res Ther. 8: R171.
Hanley, W.D. et al. (2006) Variant isoforms of CD44 are P- and L-selectin ligands on colon carcinoma cells.
FASEB J. 20: 337-9.
Shirure, V.S. et al. (2015) CD44 variant isoforms expressed by breast cancer cells are functional E-selectin ligands under flow conditions.
Am J Physiol Cell Physiol. 308 (1): C68-78.