CD44 antibody | 156-3C11

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Mouse anti Human CD44

Product Type
Monoclonal Antibody
Clone
156-3C11
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2726T C F IP P* WB 25 µg
MCA2726 C F IF IP P* WB 0.2 mg
Mouse anti Human CD44 antibody, clone 156-3C11 recognizes human Phagocytic glycoprotein 1 also known as CD44, HCAM or CD44s. CD44 is a ~90 kDa single pass type I transmembrane glycoprotein. Various isoforms of CD44 exist due to differential expression of exon products form the membrane proximal region of the extracellular domain. Mouse anti Human CD44 antibody, clone 156-3C11 recognizes the ~90 kDa standard form lacking any of the alternative spliced products, the clone is expected to recognize all isoforms of CD44. CD44 is expressed on leucocytes, erythrocytes, white matter of the brain and some epithelial cells of the breast and small intestine. Antibodies produced by clone 156-3C11 recognise epitope 3, defined as a protease resistant epitope on the CD44 molecule (CD44 and CD45R Cluster report. In Leucocyte Typing V. White cell differentiation antigens. Eds Schlossman, S.F. et al).

CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration (Lesley et al. 1990). CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing (Shimizu et al. 1989). CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential (East and Hart 1993).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Baboon
African green monkey
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Stimulated human leucocytes.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P16070 Related reagents
Entrez Gene
CD44 Related reagents
Regulatory
For research purposes only

Applications of CD44 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen 1/100
Immunohistology - Paraffin 1 1/100
Immunoprecipitation
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human tonsil.
Western Blotting
MCA2726 detects a band of approximately 80kDa in HeLa cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA929 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Application Based External Images

Western Blotting

Product Specific References

References for CD44 antibody

  1. Denning, S.M. et al. (1995) CD44 and CD45R Cluster report. In Leucocyte Typing V. White cell differentiation antigens. Eds Schlossman, S.F. et al.
    Oxford University Press. Volume 2,AS10:1713 – 1719
  2. Olsson, E. et al. (2011) CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers.
    BMC Cancer. 11: 418.
  3. Alves, C.S. et al. (2009) Biomolecular characterization of CD44-fibrin(ogen) binding: distinct molecular requirements mediate binding of standard and variant isoforms of CD44 to immobilized fibrin(ogen).
    J Biol Chem. 284: 1177-89.
  4. Heidemann, F. et al. (2014) Selectins mediate small cell lung cancer systemic metastasis.
    PLoS One. 9(4):e92327.
  5. Zhang, D. et al. (2015) Screening and Identification of a Phage Display Derived Peptide That Specifically Binds to the CD44 Protein Region Encoded by Variable Exons.
    J Biomol Screen. pii: 1087057115608604.
  6. Zhang, P. et al. (2014) CD44 variant, but not standard CD44 isoforms, mediate disassembly of endothelial VE-cadherin junction on metastatic melanoma cells.
    FEBS Lett. 588 (24): 4573-82.
  7. Pinto, F. et al. (2014) T-box transcription factor brachyury is associated with prostate cancer progression and aggressiveness.
    Clin Cancer Res. 20 (18): 4949-61.
  8. Afonso, J. et al. (2015) CD147 and MCT1-potential partners in bladder cancer aggressiveness and cisplatin resistance.
    Mol Carcinog. 54 (11): 1451-66.
  9. Lawson, J.S. et al. (2018) Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor β1.
    BMC Vet Res. 14 (1): 76.