CD44 antibody | 156-3C11
KO Validated
IP-MS Validated
Mouse anti Human CD44 antibody, clone 156-3C11 (MCA2726) used for the determination of CD44 expression on small cell lung cancer cells by flow cytometry.
Image caption:
SCLC cell line OH-1 binds to selectins and expresses CEA. OH-1 cells, as a cell line of neuroectodermal origin, are positive for NCAM and EpCAM, which could be detected by flow cytometry analysis and can thus serve as a marker for these cells when grown in mice. Cells show binding to E- and P-selectin fusion protein. The glycosylation motif sialyl Lewis A, a binding partner recognized by selectins, could be detected with the CA19-9 antibody. The cell line OH-1 exhibits several known proteins which known to be protein backbones for selectin binding carbohydrate residues such as PSGL-1, MUC18, CD44 and CEA. Isotype controls are shown as dotted lines.
From: Heidemann F, Schildt A, Schmid K, Bruns OT, Riecken K, Jung C, et al.. (2014)
Selectins Mediate Small Cell Lung Cancer Systemic Metastasis.
PLoS ONE 9(4): e92327.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD44 antibody, clone 156-3C11 (MCA2726) used for for the determination of CD44 expression on human small cell lung cancer cell (OH-1) tumors in mice by immunohistochemistry.
Image caption:
Spontaneous OH-1 metastases express CEA. Primary OH-1 tumor as well as spontaneous OH-1 lung and bone metastases were positively stained for CD44, CEA, EpCAM and PGP9.5 (red = positive cells). Metastases are indicated by red asterisks. Scale bar: 50 μm for all panels.
From: Heidemann F, Schildt A, Schmid K, Bruns OT, Riecken K, Jung C, et al. (2014)
Selectins Mediate Small Cell Lung Cancer Systemic Metastasis.
PLoS ONE 9(4): e92327.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD44 antibody, clone 156-3C11 (MCA2726) used for for the determination of CD44 expression on human small cell lung cancer cell tumors by immunohistochemistry.
Image caption:
Decreased survival of SCLC patients with moderate CEA expression levels. TMA of SCLC patients was immunohistochemically stained against CEA. Three patient groups were discriminated: (D) no CEA expression (CEA−), (E) moderate CEA expression (CEA+) and (F) strong CEA expression (CEA++). Kaplan Meier survival curves show significantly decreased median survival of patients with moderate CEA levels compared to patients with (A) negative (log-rank test, p = 0.0054) or (B) strong CEA levels (log-rank test, p = 0.0002), but no significant difference between median survival of patients (C) with negative compared to strong CEA levels (log-rank test, p = 0.1016). In parallel a TMA of SCLC was immunohistochemically stained against CD44. Two patient groups were discriminated: (H) CD44 negative (CD44−) and (I) CD44 positive (CD44+). Kaplan Meier survival curve shows no statistical different median survival of patients (G) with negative CD44 levels compared with patients with positive CD44 levels (log-rank test, p = 0.7). Scale bar = 200 μm.
From: Heidemann F, Schildt A, Schmid K, Bruns OT, Riecken K, Jung C, et al. (2014)
Selectins Mediate Small Cell Lung Cancer Systemic Metastasis.
PLoS ONE 9(4): e92327.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD44 antibody, clone 156-3C11 used for the evaluation of CD44 expression on JIMT-1 breast cancer cell lysate by western blotting.
Image caption:
Mammosphere propagation of cell lines changed the mRNA and protein expression of CD44 isoforms. A. Breast cancer cell lines grown as monolayer cells or mammospheres were analyzed for mRNA expression of CD44 isoforms using q-RT-PCR. In general, the gene expression of the alternatively spliced variants CD44v2-v10, CD44v3-v10, CD44v8-v10 increased and the expression of CD44S decreased for cells cultured as mammospheres. B. Western blot analysis (CD44 antibody, clone 156-3C11) of the breast cancer cell line JIMT-1 confirmed changes in expression of CD44 variants for cells propagated as mammospheres.
From: Olsson, E. et al.
CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers.
BMC Cancer. 2011 Sep 29;11:418.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD44 antibody, clone 156-3C11 (MCA2726) used to evaluate CD44 expression on feline renal cortical fibroblasts from healthy and diseased animals by immunofluorescence.
Image caption:
Immunofluorescence studies of feline cortical fibroblasts isolated from healthy (FCF) and diseased kidneys (CKD-FCF). Immunofluorescence staining of FCF and CKD-FCF. Cell nuclei were stained with DAPI (blue). Both CKD-FCF and FCF stained positive for the mesenchymal markers vimentin (a), CD29 (b) and CD44 (c), and were negative for the epithelial marker cytokeratin AE1/AE3 (d), endothelial cell marker vWF (e), and the myogenic marker desmin (f) (images from CKD-FCF shown). CKD-FCF demonstrated VCAM-1 expression (g) in addition to this, but FCF isolated from the kidneys of healthy cats did not (h). Isotype controls were negative (i). Images were collected using a DM4000B upright microscope with samples illuminated using an EBQ100 light source and filter cubes A4 and L5 (all from Leica Microsystems) and an AxioCam MRm monochrome camera controlled through Axiovision software version 4.8.2 (Carl Zeiss Ltd). Images are representative of cells isolated from 3 different cats (CKD-FCF) and 2 different cats (FCF)
From: Lawson JS, Syme HM, Wheeler-Jones CPD, Elliott J.
Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor β1.
BMC Vet Res. 2018 Mar 9;14(1):76.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F P WB IF IP ResetMouse anti Human CD44
- Product Type
- Monoclonal Antibody
- Clone
- 156-3C11
- Isotype
- IgG2a
- Format
- Purified
- Specificity
- CD44
Antibody Quality and Performance Guarantee
| Mouse anti Human CD44 antibody, clone 156-3C11 recognizes human Phagocytic glycoprotein 1 also known as CD44, HCAM or CD44s. CD44 is a ~90 kDa single pass type I transmembrane glycoprotein. Various isoforms of CD44 exist due to differential expression of exon products form the membrane proximal region of the extracellular domain. Mouse anti Human CD44 antibody, clone 156-3C11 recognizes the ~90 kDa standard form lacking any of the alternative spliced products, the clone is expected to recognize all isoforms of CD44. CD44 is expressed on leucocytes, erythrocytes, white matter of the brain and some epithelial cells of the breast and small intestine. Antibodies produced by clone 156-3C11 recognise epitope 3, defined as a protease resistant epitope on the CD44 molecule (CD44 and CD45R Cluster report. In Leucocyte Typing V. White cell differentiation antigens. Eds Schlossman, S.F. et al). CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration (Lesley et al. 1990). CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing (Shimizu et al. 1989). CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential (East and Hart 1993). |
|
- Target Species
- Human
- Western Blotting
- MCA2726 detects a band of approximately 80kDa in HeLa cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Baboon African green monkey Cat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Stimulated human leucocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
1/100 | |
| Immunohistology - Paraffin 1 | |
1/100 | |
| Immunoprecipitation | |
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| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Human tonsil.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for CD44 antibody
-
Denning, S.M. et al. (1995) CD44 and CD45R Cluster report. In Leucocyte Typing V. White cell differentiation antigens. Eds Schlossman, S.F. et al.
Oxford University Press. Volume 2,AS10:1713 – 1719 -
Olsson, E. et al. (2011) CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers.
BMC Cancer. 11: 418. -
Alves, C.S. et al. (2009) Biomolecular characterization of CD44-fibrin(ogen) binding: distinct molecular requirements mediate binding of standard and variant isoforms of CD44 to immobilized fibrin(ogen).
J Biol Chem. 284: 1177-89. -
Heidemann, F. et al. (2014) Selectins mediate small cell lung cancer systemic metastasis.
PLoS One. 9(4):e92327. -
Zhang, D. et al. (2015) Screening and Identification of a Phage Display Derived Peptide That Specifically Binds to the CD44 Protein Region Encoded by Variable Exons.
J Biomol Screen. pii: 1087057115608604. -
Zhang, P. et al. (2014) CD44 variant, but not standard CD44 isoforms, mediate disassembly of endothelial VE-cadherin junction on metastatic melanoma cells.
FEBS Lett. 588 (24): 4573-82. -
Pinto, F. et al. (2014) T-box transcription factor brachyury is associated with prostate cancer progression and aggressiveness.
Clin Cancer Res. 20 (18): 4949-61. -
Afonso, J. et al. (2015) CD147 and MCT1-potential partners in bladder cancer aggressiveness and cisplatin resistance.
Mol Carcinog. 54 (11): 1451-66.
View The Latest Product References
-
Lawson, J.S. et al. (2018) Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor β1.
BMC Vet Res. 14 (1): 76. -
Lawson, J.S. et al. (2019) Characterisation of Crandell-Rees Feline Kidney (CRFK) cells as mesenchymal in phenotype.
Res Vet Sci. 127: 99-102.
MCA2726
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