CD42b antibody | AK2

Product Code Applications Pack Size List Price Your Price Qty
MCA740T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IP 25 µg loader
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loader
MCA740GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IP 0.1 mg loader
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loader
MCA740
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IP 0.2 mg loader
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loader
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Mouse anti Human CD42b antibody, clone AK2 recognizes the human CD42b cell surface antigen, also known as platelet glycoprotein GP1B.

CD42b is expressed by platelets and megakaryocytes. Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA740T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
MCA740GA, MCA740: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/50 1/100
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 100ul whole blood.

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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)

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Description Mouse IgG1 Negative Control

References for CD42b antibody

  1. Ward, C.M. & Berndt, M.C. (1995) Epitope and functional characterization of the CD42 (gpIb/IX) mAb panel.
    Leucocyte Typing V. White Cell Differentiation Antigens. Volume Two. Oxford University Press, Oxford.
  2. Burgess, J.K. et al. (1998) Quinine-dependent antibodies bind a restricted set of epitopes on the glycoprotein Ib-IX complex: characterization of the epitopes.
    Blood. 92: 2366-73.
  3. Burgess, J.K. et al. (2000) Rifampicin-dependent antibodies bind a similar or identical epitope to glycoprotein IX-specific quinine-dependent antibodies.
    Blood. 95: 1988-92.
  4. Jayo, A. et al. (2010) L718P mutation in the membrane-proximal cytoplasmic tail of beta 3 promotes abnormal alpha IIb beta 3 clustering and lipid microdomain coalescence, and associates with a thrombasthenia-like phenotype.
    Haematologica. 95: 1158-66.
  5. Lova, P. et al. (2004) Contribution of protease-activated receptors 1 and 4 and glycoprotein Ib-IX-V in the G(i)-independent activation of platelet Rap1B by thrombin.
    J Biol Chem. 279: 25299-306.
  6. Shen, Y. et al. (2000) Requirement of leucine-rich repeats of glycoprotein (GP) Ibalpha for shear-dependent and static binding of von Willebrand factor to the platelet membrane GP Ib-IX-V complex.
    Blood. 95: 903-10.
  7. Wright, S.D. et al. (1993) Double heterozygosity for mutations in the platelet glycoprotein IX gene in three siblings with Bernard-Soulier syndrome.
    Blood. 81: 2339-47.
  8. Nomura, S. et al. (1995) Significance of cytokines and CD68-positive microparticles in immune thrombocytopenic purpura.
    Eur J Haematol. 55: 49-56.
  9. View The Latest Product References
  10. Speich, H.E. et al. (2008) Platelets undergo phosphorylation of Syk at Y525/526 and Y352 in response to pathophysiological shear stress.
    Am J Physiol Cell Physiol. 295: C1045-54.
  11. Balduini, A. et al (2008) Adhesive receptors, extracellular proteins and myosin IIA orchestrate proplatelet formation by human megakaryocytes.
    J Thromb Haemost. 6: 1900-7.
  12. Amor, N.B. et al. (2009) Acidic-store depletion is required for human platelet aggregation.
    Blood Coagul Fibrinolysis. 20: 511-6.
  13. Tasneem, S. et al. (2009) Platelet adhesion to multimerin 1 in vitro: influences of platelet membrane receptors, von Willebrand factor and shear.
    J Thromb Haemost. 7: 685-92.
  14. Lincoln, B. et al. (2010) Integrated system investigating shear-mediated platelet interactions with von Willebrand factor using microliters of whole blood
    Anal Biochem. 405: 174-83.
  15. Goetzl EJ et al. (2016) Human plasma platelet-derived exosomes: effects of aspirin.
    FASEB J. Feb 12. pii: fj.201500150R. [Epub ahead of print]
  16. Michalska-Jakubus, M. et al. (2016) Plasma endothelial microparticles reflect the extent of capillaroscopic alterations and correlate with the severity of skin involvement in systemic sclerosis.
    Microvasc Res. Nov 23. pii: S0026-2862(16)30097-8. [Epub ahead of print]
  17. Ralph, A. et al. (2016) Computational Tracking of Shear-Mediated Platelet Interactions with von Willebrand Factor.
    Cardiovasc Eng Technol. 7 (4): 389-405.
  18. Rossi, E. et al. (2017) Human endoglin as a potential new partner involved in platelet-endothelium interactions.
    Cell Mol Life Sci. Oct 28 [Epub ahead of print].
  19. Kim, J.S. et al. (2021) Randomization to Omega-3 Fatty Acid Supplementation and Endothelial Function in COPD: The COD-Fish Randomized Controlled Trial.
    Chronic Obstr Pulm Dis. 8(1):41-53.

Flow Cytometry

Functional Assays

Synonyms
GPIB-ALPHA
RRID
AB_323684
UniProt
P07359
Entrez Gene
GP1BA
GO Terms
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0005624 membrane fraction
GO:0007597 blood coagulation, intrinsic pathway
GO:0015057 thrombin receptor activity
GO:0030168 platelet activation
GO:0042730 fibrinolysis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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