CD42a antibody | GRP-P

Mouse anti Human CD42a

Product Type
Monoclonal Antibody
Clone
GRP-P
Isotype
IgG1
Specificity
CD42a

Product Code Applications Pack Size List Price Your Price Qty
MCA1227
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD42a antibody, clone GRP-P recognizes the platelet GPIX glycoprotein, a 23kDa surface marker expressed by platelets and megakaryocytes. Platelet GPIX is also known as CD42a.

The CD42 complex is the major platelet receptor for von Willebrand factor.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mink
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Human red blood cells and platelets.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/100
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

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References for CD42a antibody

  1. Sarma, J. et al. (2002) Increased platelet binding to circulating monocytes in acute coronary syndromes.
    Circulation. 105 (18): 2166-71.
  2. Bournazos, S. et al. (2008) Monocyte functional responsiveness after PSGL-1-mediated platelet adhesion is dependent on platelet activation status.
    Arterioscler Thromb Vasc Biol. 28: 1491-8.
  3. Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
    Vet Immunol Immunopathol. 119: 27-37.
  4. Perdomo, J. et al. (2011) Quinine-induced thrombocytopenia: drug-dependent GPIb/IX antibodies inhibit megakaryocyte and proplatelet production in vitro.
    Blood. 117: 5975-86.
  5. Harding, S.A. et al. (2004) Increased CD40 ligand and platelet-monocyte aggregates in patients with type 1 diabetes mellitus.
    Atherosclerosis. 176: 321-5.
  6. Harding, S.A. et al. (2007) Clopidogrel reduces platelet-leucocyte aggregation, monocyte activation and RANTES secretion in type 2 diabetes mellitus.
    Heart. 92: 1335-7.
  7. Eisbacher, M. et al. (2001) Inducible expression of the megakaryocyte-specific gene glycoprotein IX is mediated through an Ets binding site and involves upstream activation of extracellular signal-regulated kinase.
    Cell Growth Differ. 12: 435-45.
  8. Vettore, S. et al. (2008) Novel point mutation in a leucine-rich repeat of the GPIbalpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard-Soulier syndrome affecting two unrelated families: the N41H variant.
    Haematologica. 93: 1743-7.
  9. View The Latest Product References
  10. Fox, S.C. et al. (2004) Quantitation of platelet aggregation and microaggregate formation in whole blood by flow cytometry.
    Platelets. 15: 85-93.
  11. Din, J.N. et al. (2010) Effect of moderate walnut consumption on lipid profile, arterial stiffness and platelet activation in humans.
    Eur J Clin Nutr. 2011 Feb;65(2):234-9.
  12. Din, J.N. et al. (2008) Dietary intervention with oil rich fish reduces platelet-monocyte aggregation in man.
    Atherosclerosis. 197: 290-6.
  13. Schuberth, H.J. et al. (2007) Reactivity of cross-reacting monoclonal antibodies with canine leukocytes, platelets and erythrocytes.
    Vet Immunol Immunopathol. 119: 47-55.
  14. Tunströmer, K. et al. (2018) Quantification of Platelet Contractile Movements during Thrombus Formation.
    Thromb Haemost. 118 (9): 1600-11.
  15. Jamaly, S. et al. (2018) Elevated plasma levels of P-selectin glycoprotein ligand-1-positive microvesicles in patients with unprovoked venous thromboembolism.
    J Thromb Haemost. May 31 [Epub ahead of print].

Immunofluorescence

Synonyms
GPIX
RRID
AB_321696
UniProt
P14770
Entrez Gene
GP9
GO Terms
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0007597 blood coagulation, intrinsic pathway
GO:0030168 platelet activation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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