CD40 antibody | rA38B38-06/3H2
Mouse anti Human CD40
- Product Type
- Monoclonal Antibody
- Clone
- rA38B38-06/3H2
- Isotype
- IgG1
- Specificity
- CD40
Antibody Quality and Performance Guarantee
TZA0162GA
Recombinant Antibodies
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| Mouse anti Human CD40 antibody, clone rA38B38-06/3H2, is a recombinant monoclonal antibody that recognizes CD40, also known as Bp50 or Tumor necrosis factor receptor superfamily member 5. CD40 is a type 1 transmembrane protein belonging to the Tumor Necrosis Factor Receptor (TNFR) superfamily. It is expressed on a variety of immune cells, including B lymphocytes, dendritic cells (DCs) and monocytes. The ligand for CD40 (CD154) is predominantly expressed on the surface of activated T cells. Ligand binding to CD40 activates a range of downstream signaling pathways including the canonical and non-canonical NF-κB, PI3 kinase and MAP kinase pathways. On DCs, CD40 engagement by CD40 ligand expressing CD4+ T cells stimulates the production of proinflammatory cytokines and increases the expression of co-stimulatory molecules such as CD80. DCs are licensed to present exogenous molecules to CD8+ T cells, resulting in their activation. On B cells, CD40 engagement results in B cell activation, proliferation and differentiation. CD40 can be used as a surface marker for B cells. It is a potential therapeutic target for treatment of cancer. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant CD40 Glu21-Arg193 expressed by mammalian cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
- Synonyms
- TNFRSF5
- UniProt
- P25942
- Entrez Gene
- CD40
- GO Terms
- GO:0005576 extracellular region
- GO:0004872 receptor activity
- GO:0006461 protein complex assembly
- GO:0006954 inflammatory response
- GO:0009898 internal side of plasma membrane
- GO:0019899 enzyme binding
- GO:0030168 platelet activation
- GO:0035631 CD40 receptor complex
- GO:0042100 B cell proliferation
- View More GO Terms
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
View more products with CD40 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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