CD40 antibody | LOB7/6

Mouse anti Human CD40

Product Type
Monoclonal Antibody
Clone
LOB7/6
Isotype
IgG2a
Specificity
CD40

Product Code Applications Pack Size List Price Your Price Qty
MCA1590
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P * 0.2 mg loader
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loader
MCA1590T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P * 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD40 antibody, clone LOB7/6 recognizes the human CD40 cell surface antigen, a 48kDa glycoprotein expressed by B lymphocytes and weakly by some monocytes.

CD40 is involved in the process of B cell selection in germinal centres and is vital in T cell-B cell interactions.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Tris buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
CD40 Ig(Fc) fusion protein containing the EC region of human CD40 and Fc region of human IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 20ug/ml
Immunohistology - Frozen 1ug/ml 10ug/ml
Immunohistology - Paraffin 1 1ug/ml 10ug/ml
Immunoprecipitation
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Human Tonsil

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References for CD40 antibody

  1. Quadbeck, B. et al. (2002) Maturation of thyroidal dendritic cells in Graves' disease.
    Scand J Immunol. 55 (6): 612-20.
  2. Kirsch, B. M. et al . (2005) The active metabolite of leflunomide, A77 1726, interferes with dendritic cell function.
    Arthritis Res. Ther. 7: R694-R703.
  3. Cheadle, E. et al. (2003) Mycobacterium bovis bacillus Calmette-Guerin-infected dendritic cells potently activate autologous T cells via a B7 and interleukin-12-dependent mechanism.
    Immunology.108: 79-88.
  4. Carpenter, E.L. et al. (2009) Activation of human B cells by the agonist CD40 antibody CP-870,893 and augmentation with simultaneous toll-like receptor 9 stimulation.
    J Transl Med. 7: 93.
  5. Garcia-Nieto, S. et al. (2010) Laminin and Fibronectin Treatment Leads to Generation of Dendritic Cells with Superior Endocytic Capacity.
    PLoS ONE. 5: 1-10.
  6. Wang, Y.S. et al. (2007) Characterization of canine monocyte-derived dendritic cells with phenotypic and functional differentiation.
    Can J Vet Res. 71: 165-74.
  7. Vlachoyiannopoulos, P.G. et al. (2004) Anti-CD40 antibodies in antiphospholipid syndrome and systemic lupus erythematosus.
    Thromb Haemost. 92: 1303-11.
  8. Leigh, J.E. et al. (2006) Characterization of the immune status of CD8+ T cells in oral lesions of human immunodeficiency virus-infected persons with oropharyngeal Candidiasis.
    Clin Vaccine Immunol. 13: 678-83.
  9. View The Latest Product References
  10. Newman, K.C. et al. (2006) Cross-talk with myeloid accessory cells regulates human natural killer cell interferon-gamma responses to malaria.
    PLoS Pathog. 2: e118.
  11. Kuijf, M.L. et al. (2010) TLR4-mediated sensing of Campylobacter jejuni by dendritic cells is determined by sialylation.
    J Immunol. 185: 748-55.
  12. Huizinga R et al. (2015) Innate Immunity to Campylobacter jejuni in Guillain-Barré Syndrome.
    Ann Neurol. 78 (3): 343-54.
  13. Yıldırım C et al. (2015) Galectin-2 induces a proinflammatory, anti-arteriogenic phenotype in monocytes and macrophages.
    PLoS One. 10 (4): e0124347.
  14. Brencicova, E. et al. (2017) Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma.
    PLoS One. 12 (4): e0175712.
  15. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
    J Biomed Biotechnol. 2012: 172420.
  16. Tischer, S. et al. (2011) Heat shock protein 70/peptide complexes: potent mediators for the generation of antiviral T cells particularly with regard to low precursor frequencies.
    J Transl Med. 9: 175.
  17. Unosson, J. et al. (2021) Acute cardiovascular effects of controlled exposure to dilute Petrodiesel and biodiesel exhaust in healthy volunteers: a crossover study.
    Part Fibre Toxicol. 18 (1): 22.
  18. Milhau, N. et al. (2020) In vitro. evaluations on canine monocyte-derived dendritic cells of a nanoparticles delivery system for vaccine antigen against Echinococcus granulosus..
    PLoS One. 15 (2): e0229121.
  19. Uetz-von Allmen, E et al. (2021) CAL-1 as Cellular Model System to Study CCR7-Guided Human Dendritic Cell Migration.
    Front Immunol. 12: 702453.

Flow Cytometry

Immunohistology - Frozen

Immunoprecipitation

RRID
AB_321685
UniProt
P25942
Entrez Gene
CD40
GO Terms
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006461 protein complex assembly
GO:0006954 inflammatory response
GO:0009898 internal side of plasma membrane
GO:0019899 enzyme binding
GO:0030168 platelet activation
GO:0035631 CD40 receptor complex
GO:0042100 B cell proliferation
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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