CD39 antibody | rA8-07/4C11
Mouse anti Human CD39
- Product Type
- Monoclonal Antibody
- Clone
- rA8-07/4C11
- Isotype
- IgG1
- Specificity
- CD39
Antibody Quality and Performance Guarantee
TZA082GA
No associated image currently available
| Mouse anti Human CD39 antibody, clone rA8-07/4C11, is a recombinant monoclonal antibody that recognizes CD39, also known as Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) or NTPDase1. CD39 is an integral membrane protein that dephosphorylates extracellular ATP (eATP) to AMP. CD39 is the rate limiting enzyme in the generation of extracellular adenosine. Accumulated extracellular adenosine mediates broad immunosuppressive actions by binding adenosine receptors such as A2AR. CD39 is expressed on several immune subpopulations, particularly B cells, natural killer (NK) cells, tumor-associated-macrophages (TAM) and tumor-infiltrating lymphocytes (TILs) including Tregs. It is also highly expressed in placenta, lung, skeletal muscle and kidney. CD39 can be used as a marker to distinguish Tregs from other resting or activated T cells. Overexpression of CD39 has been found in several cancers, including ovarian cancer, breast cancer, lymphoma, colon cancer and melanoma, with high expression levels considered an indicator of poor disease outcomes and disease progression. The upregulation of CD39 by tumors is therefore implicated in depleting the immune-stimulatory eATP in the tumor microenvironment (TME), leading to tumor immune evasion. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Mammalian cells transfected with full length CD39
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
- Synonyms
- ENTPD1
- UniProt
- P49961
- Entrez Gene
- ENTPD1
- GO Terms
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0005887 integral to plasma membrane
- GO:0005524 ATP binding
- GO:0016787 hydrolase activity
View more products with CD39 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up