CD366 antibody | rA20B20-03/4A3
Mouse anti Human CD366
- Product Type
- Monoclonal Antibody
- Clone
- rA20B20-03/4A3
- Isotype
- IgG1
- Specificity
- CD366
Antibody Quality and Performance Guarantee
TZA0122GA
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| Mouse anti Human CD366 antibody, clone rA20B20-03/4A3, is a recombinant monoclonal antibody that recognizes CD366, also known as T-cell immunoglobulin mucin family member 3 (TIM-3). CD366 is a type I transmembrane glycoprotein encoded by the HAVCR2 gene and part of the TIM family of proteins. It is expressed on the surface of Th1, Th17, Tc1 and Treg cells, natural killer cells, dendritic cells, monocytes, mast cells and macrophages. CD366 has a role in regulating immune responses by managing the balance between immune activation and tolerance. Interaction between CD366 and the first identified ligand, Galectin-9, results in Th1 cell death. Other CD366 ligands include carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), phosphatidyl serine (PtdSer), and high-mobility group protein B1 (HMGB1). CD366 is characterised as a marker of T cell exhaustion. It has been reported to be highly expressed in cancer tissues and is used as a negative prognostic biomarker for several types of tumor. CD366 has been identified as a potential therapeutic target in cancer immunotherapy and the treatment of sepsis. CD366 dysregulation is also related to the development of autoimmune diseases. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant CD366 Ser22-Gly202 expressed by mammalian cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
- Synonyms
- TIM-3
- UniProt
- Q8TDQ0
- Entrez Gene
- HAVCR2
- GO Terms
- GO:0016021 integral to membrane
TZA0122GA
64744245
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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