CD35 antibody | E11

Mouse anti Human CD35

Product Type
Monoclonal Antibody
Clone
E11
Isotype
IgG1
Specificity
CD35

Product Code Applications Pack Size List Price Your Price Qty
MCA554
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * WB 0.2 mg loader
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loader
MCA554GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD35 antibody, clone E11 recognizes complement receptor type 1, also known as CD35 or the C3b/C4b receptor. CD35 a single chain, type I single pass transmembrane glycoprotein which exists in four allotypic forms (A,B,C,D) of ~190 kDa, ~220 kDa, ~160 kDa and ~250 kDa respectively (Khera et al. 2009).

CD35 is expressed by granulocytes, monocytes, B cells and some T cells. Functionally, CD35 acts as a receptor for the C3b component of complement.

Mouse anti Human CD35 antibody, clone E11 is suitable for use in Western Blotting (Birmingham et al. 1996).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
Baboon
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human acute monocytic leukaemia cells.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen 1ug/ml 10ug/ml
Immunohistology - Paraffin 1 1ug/ml 10ug/ml
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Histology Positive Control Tissue
Human Tonsil

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References for CD35 antibody

  1. Hogg, N. et al. (1984) Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1).
    Eur J Immunol. 14 (3): 236-43.
  2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  3. Birmingham, D.J. et al. (1996) The baboon erythrocyte complement receptor is a glycophosphatidylinositol-linked protein encoded by a homologue of the human CR1-like genetic element.
    J Immunol. 157 (6): 2586-92.
  4. Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
    Cytometry. 29 (4): 351-62.
  5. Weissgerber P et al. (2003) Investigation of mechanisms involved in phagocytosis of Legionella pneumophila by human cells.
    FEMS Microbiol Lett. 219 (2): 173-9.
  6. Schmidt, C.Q. et al. (2016) Selectivity of C3-opsonin targeted complement inhibitors: A distinct advantage in the protection of erythrocytes from paroxysmal nocturnal hemoglobinuria patients.
    Immunobiology. 221 (4): 503-11.
  7. Noda, G.S. et al. () Specificities and isotypes of erythrocytes autoantibodies in patients with warm autoimmune hemolytic anemia
    Rev Cubana Hematol Inmunol Hemoter 36(4): e1283.

Synonyms
CR1
RRID
AB_321673
UniProt
P17927
Entrez Gene
CR1
GO Terms
GO:0004875 complement receptor activity
GO:0005887 integral to plasma membrane
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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