CD35 antibody | E11
Mouse anti Human CD35
- Product Type
- Monoclonal Antibody
- Clone
- E11
- Isotype
- IgG1
- Specificity
- CD35
Antibody Quality and Performance Guarantee
| Mouse anti Human CD35 antibody, clone E11 recognizes complement receptor type 1, also known as CD35 or the C3b/C4b receptor. CD35 a single chain, type I single pass transmembrane glycoprotein which exists in four allotypic forms (A,B,C,D) of ~190 kDa, ~220 kDa, ~160 kDa and ~250 kDa respectively (Khera et al. 2009). CD35 is expressed by granulocytes, monocytes, B cells and some T cells. Functionally, CD35 acts as a receptor for the C3b component of complement. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey Baboon - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Human acute monocytic leukaemia cells.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
| Immunohistology - Frozen | |
1ug/ml | 10ug/ml |
| Immunohistology - Paraffin 1 | |
1ug/ml | 10ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Histology Positive Control Tissue
- Human Tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Hogg, N. et al. (1984) Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1).
Eur J Immunol. 14 (3): 236-43.
References for CD35 antibody
-
Birmingham, D.J. et al. (1996) The baboon erythrocyte complement receptor is a glycophosphatidylinositol-linked protein encoded by a homologue of the human CR1-like genetic element.
J Immunol. 157 (6): 2586-92. -
Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
Cytometry. 29 (4): 351-62. -
Weissgerber P et al. (2003) Investigation of mechanisms involved in phagocytosis of Legionella pneumophila by human cells.
FEMS Microbiol Lett. 219 (2): 173-9. -
Schmidt, C.Q. et al. (2016) Selectivity of C3-opsonin targeted complement inhibitors: A distinct advantage in the protection of erythrocytes from paroxysmal nocturnal hemoglobinuria patients.
Immunobiology. 221 (4): 503-11. -
Noda, G.S. et al. (2020) Specificities and isotypes of erythrocytes autoantibodies in patients with warm autoimmune hemolytic anemia
Rev Cubana Hematol Inmunol Hemoter 36(4): e1283. -
Klaas, M. et al. (2023) Sialoadhesin (CD169/Siglec-1) is an extended molecule that escapes inhibitory cis-interactions and synergizes with other macrophage receptors to promote phagocytosis.
Glycoconj J. 40 (2): 213-23. -
Wagstaffe, H.R. et al. (2023) The Development of Immunological Assays to Evaluate the Level and Function of Antibodies Induced by Klebsiella pneumoniae O-Antigen Vaccines.
mSphere. : e0068022.
- Synonyms
- CR1
- RRID
- AB_321673
- UniProt
- P17927
- Entrez Gene
- CR1
- GO Terms
- GO:0004875 complement receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
MCA554GA
1013
154520
MCA554
1612
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