CD335 antibody | 9E2
No associated image currently available
Mouse anti Human CD335
- Product Type
- Monoclonal Antibody
- Clone
- 9E2
- Isotype
- IgG1
- Specificity
- CD335
Antibody Quality and Performance Guarantee
| Mouse anti Human CD335 antibody, clone 9E2 recognizes human NK (natural killer) cell-specific marker CD335, otherwise known as NKp46, a 46kDa type I transmembrane glycoprotein, and the first identified member of the natural cytotoxicity receptor (NCR) family. CD335 is uniquely expressed by all resting and activated NK cells, and considered to be the major NK cell lysis receptor for autologous pathogen-infected and tumour target cells, during natural cytotoxicity responses. Ligands of CD335 include viral haemagglutinins (HAs), and heparan sulphate proteoglycans (HSPGs) on the surface of tumour cells. Mouse anti Human CD335 antibody, clone 9E2 mediates re-directed NK cell cytotoxicity (Yusa et al. 2002, Chen et al. 2007). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- CD335-Fc fusion protein
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
References for CD335 antibody
-
Yusa, S. et al. (2002) SHP-1- and phosphotyrosine-independent inhibitory signaling by a killer cell Ig-like receptor cytoplasmic domain in human NK cells.
J Immunol. 168 (10): 5047-57. -
Chklovskaia, E. et al. (2004) Reconstitution of dendritic and natural killer-cell subsets after allogeneic stem cell transplantation: effects of endogenous flt3 ligand.
Blood. 103 (10): 3860-8. -
Chen, Y. et al. (2007) Prostaglandin D2 suppresses human NK cell function via signaling through D prostanoid receptor.
J Immunol. 179 (5): 2766-73.
- Synonyms
- NKp46
- RRID
- AB_1720030
- UniProt
- O76036
- Entrez Gene
- NCR1
- GO Terms
- GO:0004872 receptor activity
- GO:0005057 receptor signaling protein activity
- GO:0005887 integral to plasma membrane
- GO:0006968 cellular defense response
- GO:0016514 SWI/SNF complex
- GO:0030101 natural killer cell activation
- GO:0042269 regulation of natural killer cell mediated cytotoxicity
MCA4646GA
151722
163712
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CD335 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up