CD33 antibody | rWM53
Mouse anti Human CD33
- Product Type
- Monoclonal Antibody
- Clone
- rWM53
- Isotype
- IgG1
- Specificity
- CD33
Antibody Quality and Performance Guarantee
TZA1271GA
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| Mouse anti Human CD33, clone rWM53, is a recombinant monoclonal antibody that recognizes CD33, also known as Siglec-3 or gp67. CD33 is a single pass type I membrane protein and member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells, but is absent on lymphocytes, normal platelets, hematopoietic stem cells and erythrocytes. CD33 binds the sialoglycan sequence Neu5Acα2-3[6SO3]Galβ1-4GlcNAc on the surface of target cells. Upon CD33 activation, the immunoreceptor tyrosine-based inhibition motif (ITIM) is phosphorylated by Src family kinases, resulting in recruitment of SH2 domain containing phosphatases and inhibition of cellular activation. As sialoglycans are present on almost all human cells, CD33 and other members of the siglec family have a role in discriminating self from non-self. CD33 has a role in autoimmune diseases and is a drug target in acute myeloid leukemia. Mouse anti Human CD33 antibody, clone rWM53, is derived from clone WM53 (MCA1271), generated by cloning the variable DNA sequences into expression vectors for recombinant protein expression. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Human AML cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
Source Hybridoma Reference
-
Favaloro, E.J. et al. (1987) Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33).
Dis Markers. 5 (4): 215-25.
- Synonyms
- GP67
- UniProt
- P20138
- Entrez Gene
- CD33
- GO Terms
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
- GO:0007267 cell-cell signaling
- GO:0008285 negative regulation of cell proliferation
View more products with CD33 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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