CD326 antibody | VU-1D9
Mouse anti Human CD326 antibody, cloneVU-1D9 (MCA1870G) used to identify CD326 expressing cells in circulating tumor cells by immunofluorescence.
Image caption:
Immunofluorescent micrographs of CTCs expressing bone alkaline phosphatase (B-ALP, or BAP), Epithelial cell adhesion molecular (EpCAM), DAPI, and CD45.
A. Expression of B-ALP and EpCAM in negative and positive control cell lines spiked into healthy volunteer blood. HELA cells positive for B-ALP (Green) and negative for EpCAM (Yellow) are shown on the left and T47D cells negative for B-ALP and positive for EpCAM are shown on the right. B. Changes in CTC B-ALP expression over time during radium-223 therapy. C. Examples of CTCs from men with bone metastatic CRPC treated with radium-223, demonstrating heterogeneity of CTC B-ALP expression in CTCs including in EpCAM negative CTCs. D. Plots of B-ALP (+) CTCs in healthy volunteers (n = 10) and patients (n = 20) over time, as well as Cellsearch CTCs (n = 20) from patients over time during radium-223 therapy (baseline, month 3 and month 6). *p = 0.0053 for difference in B-ALP (+) CTCs between healthy volunteers and mCRPC patients by non-parametric Kruskal-Wallis test.
From: Armstrong AJ, Gupta S, Healy P, Kemeny G, Leith B, Zalutsky MR, et al. (2019)
Pharmacodynamic study of radium-223 in men with bone metastatic castration resistant prostate cancer.
PLoS ONE 1 (5):e0216934.
https://doi.org/10.1371/journal.pone.0216934
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD326
- Product Type
- Monoclonal Antibody
- Clone
- VU-1D9
- Isotype
- IgG1
- Specificity
- CD326
Antibody Quality and Performance Guarantee
| Mouse anti Human CD326 antibody, clone VU-1D9 recognizes epithelial cell adhesion molecule (Ep-CAM), a ~34 kDa cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4. CD326 is a type 1 transmembrane glycoprotein, expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium. Mouse anti Human CD326 antibody, clone VU-1D9, has been validated for use on the Genesis Cell Isolation System with the CelSelect SlideTM technology. |
|
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- HG9 cell line (small cell lung carcinoma)
- Approx. Protein Concentrations
- 1 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin 1 | |
1/200 | |
| Immunoprecipitation | |
||
| Western Blotting | |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
- Histology Positive Control Tissue
- Human large bowel
- Western Blotting
- Mouse anti human CD326 antibody, clone VU-1D9 detects a band of approximately 38kDa in HT-29 cell lysates
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD326 antibody
-
Koga, Y. et al. (2008) Improved recovery of exfoliated colonocytes from feces using newly developed immunomagnetic beads.
Gastroenterol Res Pract. 2008: 605273. -
Fillmore, C.M. et al. (2010) Estrogen expands breast cancer stem-like cells through paracrine FGF/Tbx3 signaling.
Proc Natl Acad Sci U S A. 107 (50): 21737-42. -
Murakata, A. et al. (2011) Gene expression signature of the gross morphology in hepatocellular carcinoma.
Ann Surg. 253: 94-100. -
Keller, P.J. et al. (2012) Defining the cellular precursors to human breast cancer.
Proc Natl Acad Sci U S A. 109 (8): 2772-7. -
Jeong, H.T. et al. (2012) MRI features of hepatocellular carcinoma expressing progenitor cell markers.
Liver Int. 32: 430-40. -
Armstrong, A.J. et al. (2019) Pharmacodynamic study of radium-223 in men with bone metastatic castration resistant prostate cancer.
PLoS One. 14 (5): e0216934.
Further Reading
-
Winter, M.J. et al. (2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.
Am J Pathol. 163 (6): 2139-48.
- Synonyms
- Ep-CAM
- RRID
- AB_931706
- UniProt
- P16422
- Entrez Gene
- EPCAM
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005923 tight junction
- GO:0008284 positive regulation of cell proliferation
- GO:0016323 basolateral plasma membrane
- GO:0016324 apical plasma membrane
- GO:0016328 lateral plasma membrane
MCA1870GT
150396
151424
MCA1870G
0412R
158126
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