CD326 antibody | VU-1D9

Mouse anti Human CD326

Product Type
Monoclonal Antibody
Clone
VU-1D9
Isotype
IgG1
Specificity
CD326

Product Code Applications Pack Size List Price Your Price Qty
MCA1870G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P * WB 0.2 mg loader
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loader
MCA1870GT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P * WB 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD326 antibody, clone VU-1D9 recognizes epithelial cell adhesion molecule (Ep-CAM), a ~34 kDa cell surface antigen otherwise known as CD326, MOC31 or Ber-EP4. CD326 is a type 1 transmembrane glycoprotein, expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.

Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
HG9 cell line (small cell lung carcinoma)
Approx. Protein Concentrations
1 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/200
Immunoprecipitation
Western Blotting
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Large Bowel
Western Blotting
MCA1870G detects a band of approximately 38kDa in HT-29 cell lysates

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References for CD326 antibody

  1. Koga, Y. et al. (2008) Improved recovery of exfoliated colonocytes from feces using newly developed immunomagnetic beads.
    Gastroenterol Res Pract. 2008: 605273.
  2. Fillmore, C.M. et al. (2010) Estrogen expands breast cancer stem-like cells through paracrine FGF/Tbx3 signaling.
    Proc Natl Acad Sci U S A. 107 (50): 21737-42.
  3. Murakata, A. et al. (2011) Gene expression signature of the gross morphology in hepatocellular carcinoma.
    Ann Surg. 253: 94-100.
  4. Keller, P.J. et al. (2012) Defining the cellular precursors to human breast cancer.
    Proc Natl Acad Sci U S A. 109 (8): 2772-7.
  5. Jeong, H.T. et al. (2012) MRI features of hepatocellular carcinoma expressing progenitor cell markers.
    Liver Int. 32: 430-40.
  6. Armstrong, A.J. et al. (2019) Pharmacodynamic study of radium-223 in men with bone metastatic castration resistant prostate cancer.
    PLoS One. 14 (5): e0216934.

Further Reading

  1. Winter, M.J. et al. (2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology.
    Am J Pathol. 163 (6): 2139-48.

Flow Cytometry

Synonyms
Ep-CAM
RRID
AB_931706
UniProt
P16422
Entrez Gene
EPCAM
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005923 tight junction
GO:0008284 positive regulation of cell proliferation
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0016328 lateral plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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