CD305 antibody | NKTA255
Mouse anti Human CD305
- Product Type
- Monoclonal Antibody
- Clone
- NKTA255
- Isotype
- IgG1
- Specificity
- CD305
Antibody Quality and Performance Guarantee
| Mouse anti Human CD305 antibody, clone NKTA255 recognizes human leukocyte-associated Ig-like receptor-1 (LAIR-1), a ~40 kDa cell surface molecule that functions as an inhibitory receptor on natural killer cells, monocytes and T cells. LAIR-1, otherwise known as CD305, is also expressed by other hematopoietic cells including B cells, but is not expressed by non-haematopoietic, platelets or erythrocytes. Mouse anti Human CD305 antibody, clone NKTA255 is reported to inhibit NK cell triggering via CD16 molecules (Poggi et al. 1995). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- NK cell lines B12.100 and AM.25
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the P3U1 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunoprecipitation | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- Western Blotting
- Mouse anti Human CD305 antibody, clone NKTA255 detects a band of approximately 40 kDa
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Poggi, A. et al. (1995) p40, a novel surface molecule involved in the regulation of the non-major histocompatibility complex-restricted cytolytic activity in humans.
Eur J Immunol. 25 (2): 369-76.
References for CD305 antibody
-
Poggi, A. et al. (1997) p40 molecule regulates NK cell activation mediated by NK receptors for HLA class I antigens and TCR-mediated triggering of T lymphocytes.
Int Immunol. 9 (9): 1271-9. -
Poggi, A. et al. (2000) Engagement of the leukocyte-associated Ig-like receptor-1 induces programmed cell death and prevents NF-kappaB nuclear translocation in human myeloid leukemias.
Eur J Immunol. 30 (10): 2751-8. -
Zocchi, M.R. et al. (2001) Leukocyte-associated Ig-like receptor-1 prevents granulocyte-monocyte colony stimulating factor-dependent proliferation and Akt1/PKB alpha activation in primary acute myeloid leukemia cells.
Eur J Immunol. 31 (12): 3667-75. -
Poggi, A. et al. (2008) Lack of the leukocyte-associated Ig-like receptor-1 expression in high-risk chronic lymphocytic leukaemia results in the absence of a negative signal regulating kinase activation and cell division.
Leukemia. 22 (5): 980-8.
Further Reading
-
Meyaard, L. (2008) The inhibitory collagen receptor LAIR-1 (CD305).
J Leukoc Biol. 83 (4): 799-803.
- Synonyms
- LAIR-1
- RRID
- AB_323947
- UniProt
- Q6GTX8
- Entrez Gene
- LAIR1
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
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