CD3 antibody | UCHT1
FITC conjugated Mouse anti Human CD3 antibody, clone UCHT1 used for the evaluation of CD3 expression on human PBMC by flow cytometry.
Image caption:
Expression of RANTES in obese subjects. (a) Western blot analysis of RANTES expression in PBMCs from lean and obese subjects. The bands reacting with anti-RANTES antibody were quantified as described in Section 2 and the relative intensity was determined after correction with Actin that was used as internal control to monitor loading efficiency. The blots shown are representative of three independent experiments with consistent results. The data are presented in the form of a bar graph on the right of the figure as fold changes of RANTES protein expression in obese compared to lean subjects. (b) Characterization of the monocyte subpopulations and T cells in peripheral blood from lean and obese participants. Monocytes subsets were defined by staining for CD14 (PE), T cells by CD3 (FITC), and expression of intracellular RANTES (APC) was analyzed. Left and right upper panels are representative dot plots of CD3 and RANTES expression on/in T cells from lean and obese participants, respectively. Left and right lowest panels are representative dot plots of CD14 and RANTES expression on/in monocyte subsets from lean and obese participants, respectively. The double-positive populations (i.e., CD3+RANTES+, CD14+RANTES+, and CD14++RANTES+) were analyzed for mean RANTES fluorescence intensity.
From: Engin Baturcam, Jehad Abubaker, Ali Tiss, et al.
“Physical Exercise Reduces the Expression of RANTES and Its CCR5 Receptor in the Adipose Tissue of Obese Humans,”
Mediators of Inflammation, vol. 2014, Article ID 627150, 13 pages, 2014.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Human CD3 antibody, clone UCHT1 used for the evaluation of CD3 expression on human PBMC by flow cytometry.
Image caption:
Differential regulation of CCR5 in PBMCs and adipose tissue is associated with obesity. (c) Characterization of the monocyte subpopulations and T cells in peripheral blood from lean and obese subjects. Monocytes subsets were defined by staining for CD14 (PE), T cells by CD3 (FITC), and expression of CCR5 (APC) and then were analyzed. Gates P4 and P3 define the CD14+ and CD14++ subsets, respectively. Left and right upper panels are representative dot plots of CD3 and CCR5 expression on T cells from lean and obese subjects, respectively. Left and right lower panels are representative dot plots of CD14 and RANTES expression on monocyte subsets from lean and obese participants, respectively. The double-positive populations (i.e., CD3+CCR5+, CD14+CCR5+, and CD14++CCR5+) were analyzed for mean CCR5 fluorescence intensity. For each experiment, the sample size from each group is indicated by n.
From: Engin Baturcam, Jehad Abubaker, Ali Tiss, et al.
“Physical Exercise Reduces the Expression of RANTES and Its CCR5 Receptor in the Adipose Tissue of Obese Humans,”
Mediators of Inflammation, vol. 2014, Article ID 627150, 13 pages, 2014.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD3:Alexa Fluor® 488
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3:Alexa Fluor® 647
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3:APC
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3:FITC
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3:Low Endotoxin
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3:Pacific Blue®
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3:RPE
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3:RPE-Alexa Fluor® 647
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3:RPE-Alexa Fluor® 750
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3:RPE-Cy5
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3:RPE-Cy5.5
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3:Alexa Fluor® 700
CD3 antibody, clone UCHT1 recognizes the ectodomain of human CD3e, a 21kDa transmembrane protein. Deficiency of CD3e contributes to blocking T-cell development. It has been used successfully for the activation of human blood lymphocytes.
Mouse anti Human CD3:Starbright Blue 700
Mouse anti Human CD3:Starbright Violet 515
- Product Type
- Monoclonal Antibody
- Clone
- UCHT1
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA463A488 | F | 100 Tests/1ml |
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| MCA463A647T | F | 25 Tests/0.25ml |
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| MCA463A647 | F | 100 Tests/1ml |
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| MCA463APC | F | 100 Tests |
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| MCA463FT | F | 25 µg |
|
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| MCA463F | F | 0.1 mg |
|
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| MCA463EL | C F FN | 0.5 mg |
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| MCA463PB | F | 100 Tests/1ml |
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| MCA463GT | C F | 25 µg |
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| MCA463G | C F | 0.2 mg |
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| MCA463PET | F | 25 Tests |
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| MCA463PE | F | 100 Tests |
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| MCA463P647 | F | 100 Tests/1ml |
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| MCA463P750 | F | 100 Tests/1ml |
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| MCA463C | F | 100 Tests/1ml |
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| MCA463PECY5.5 | F | 100 Tests |
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| MCA463A700 | F | 100 Tests/1ml |
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| MCA463SBB700 | F | 100 Tests/0.5ml |
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| MCA463SBV515 | F | 100 Tests/0.5ml |
|
Mouse anti Human CD3 antibody, clone UCHT1 recognizes the human T-cell surface glycoprotein CD3 epsilon chain, also known as T-cell surface antigen T3/Leu-4 epsilon chain or CD3ε. CD3ε is a 207 amino acid, ~21kDa single pass type 1 transmembrane protein containing a single Ig-like and a single ITAM domain. Mouse anti Human CD3 antibody, clone UCHT1 was originally described as only binding to CD3ε when complexed with either the CD3δ or CD3γ subunits, as indicated by co-transfection immunofluorescence studies on COS cells (Salmerón et al. 1991). Mouse anti Human CD3 antibody, clone UCHT1 binds to a region in the ectodomain of human CD3ε and has been shown to bind to a discontinuous epitope near an acidic region of CD3ε opposite the dimer interface; as shown by crystallographic studies of the CD3ε/δ dimer complexed with a single chain UCHT1 antibody fragment (Arnett et al. 2004).
CD3 is expressed by all T lymphocytes and is seen in all lymphoid organs including lymph nodes and spleen. It is involved in thymocyte differentiation (Brodeur et al. 2009). Deficiency of the CD3ε chain contributes to blocking T-cell development and presentation of a severe combined immunodeficiency phenotype (Fischer et al. 2005).
Mouse anti Human CD3 antibody, clone UCHT1 has been used successfully for the activation of human peripheral blood lymphocytes by cross linking and subsequently for CD3ε surface espression by flow cytometry (Hirsh and Cohen 2006).
CD3 is expressed by all T lymphocytes and is seen in all lymphoid organs including lymph nodes and spleen. It is involved in thymocyte differentiation (Brodeur et al. 2009). Deficiency of the CD3ε chain contributes to blocking T-cell development and presentation of a severe combined immunodeficiency phenotype (Fischer et al. 2005).
Mouse anti Human CD3 antibody, clone UCHT1 has been used successfully for the activation of human peripheral blood lymphocytes by cross linking and subsequently for CD3ε surface espression by flow cytometry (Hirsh and Cohen 2006).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chimpanzee - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG - conjugated to Alexa Fluor®647 - liquid
- Product Form
- Purified IgG conjugated to allophycocyanin (APC) - lyophilised
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Pacific Blue® - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin - Alexa Fluor®647 - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin - Alexa Fluor® 750 - lyophilized
- Product Form
- Purified IgG conjugated to RPE-Cy5 - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin-Cy5.5 (RPE-Cy5.5) - lyophilized
- Product Form
- Purified IgG conjugated to Alexa Fluor® 700 - liquid
- Product Form
- Purified IgG conjugated to StarBright Blue 700 - liquid
- Product Form
- Purified IgG conjugated to StarBright Violet 515 - liquid
- Reconstitution
- Reconstitute with 1 ml distilled water
- Reconstitution
- Pack Size: 25 TestsReconstitute in 0.25 ml disilled waterPack Size: 100 TestsReconstitute with 1 ml distilled water
- Reconstitution
- Reconstitute with 1 ml distilled water
- Reconstitution
- Reconstitute with 1 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution - Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
Stabilizing agent - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350 - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350 - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P07766
- Entrez Gene
- CD3E
- GO Terms
- GO:0004888 transmembrane receptor activity
- GO:0005057 receptor signaling protein activity
- GO:0005887 integral to plasma membrane
- GO:0007172 signal complex assembly
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0017124 SH3 domain binding
- GO:0019901 protein kinase binding
- GO:0030159 receptor signaling complex scaffold activity
- GO:0031295 T cell costimulation
- GO:0042608 T cell receptor binding
- GO:0046982 protein heterodimerization activity
- GO:0050852 T cell receptor signaling pathway
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- Cy® and CyDye® are registered trademarks of GE Healthcare
- Acknowledgements
- Cy® and CyDye® are registered trademarks of GE Healthcare
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Regulatory
- For research purposes only
Applications of CD3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/4 | |
| Flow Cytometry | Neat | 1/2 | |
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/10 | ||
| Flow Cytometry | 1/50 | 1/100 | |
| Functional Assays | |||
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/100 | 1/200 | |
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 100ul of whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul of lysed whole peripheral blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
|
|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
|
|
| Mouse IgG1 Negative Control:APC | MCA928APC | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | C F | 0.5 mg |
|
|
| Mouse IgG1 Negative Control:Pacific Blue® | MCA928PB | F | 100 Tests/1ml |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE-Alexa Fluor® 647 | MCA928P647 | F | 100 Tests/1ml |
|
|
| Mouse IgG1 Negative Control:RPE-Alexa Fluor® 750 | MCA928P750 | F | 100 Tests/1ml |
|
|
| Mouse IgG1 Negative Control:Alexa Fluor® 700 | MCA928A700 | F | 100 Tests/1ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD3 antibody
-
Beverley, P.C. & Callard, R.E. (1981) Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.
Eur J Immunol. 11 (4): 329-34. -
Kung, P. et al. (1979) Monoclonal antibodies defining distinctive human T cell surface antigens.
Science. 206: 347-9. -
Clevers, H. et al. (1988) The transmembrane orientation of the epsilon chain of the TcR/CD3 complex.
Eur J Immunol. 18 (5): 705-10. -
Salmerón, A. et al. (1991) A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.
J Immunol. 147: 3047-52. -
Hirsh, M.I. and Cohen, V. (2006) Chloroquine prevents T lymphocyte suppression induced by anthrax lethal toxin.
J Infect Dis. 194: 1003-7. -
Mahon, N.G. et al. (2002) Immunohistologic evidence of myocardial disease in apparently healthy relatives of patients with dilated cardiomyopathy.
J Am Coll Cardiol. 39: 455-62. -
Campana, D. et al. (1987) The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage.
J Immunol. 138 (2): 648-55. -
Lawson, C.A. et al. (2006) Early rheumatoid arthritis is associated with a deficit in the CD4+CD25high regulatory T cell population in peripheral blood.
Rheumatology (Oxford). 45: 1210-7. -
Battaglia, A. et al. (2003) Lymphocyte populations in human lymph nodes. Alterations in CD4+ CD25+ T regulatory cell phenotype and T-cell receptor Vbeta repertoire.
Immunology. 110: 304-12. -
McIntosh, R.S. et al. (1997) Analysis of the T cell receptor V alpha repertoire in Hashimoto's thyroiditis: evidence for the restricted accumulation of CD8+ T cells in the absence of CD4+ T cell restriction.
J Clin Endocrinol Metab. 82: 1140-6. -
Aird, I.A. et al. (1999) Leukocytes in semen from men with spinal cord injuries.
Fertil Steril. 72: 97-103. -
Colić, M. et al. (2006) Correlation between phenotypic characteristics of mononuclear cells isolated from human periapical lesions and their in vitro production of Th1 and Th2 cytokines.
Arch Oral Biol. 51: 1120-30. -
Oliver, B. et al. (2001) Low-dose theophylline does not exert its anti-inflammatory effects in mild asthma through upregulation of interleukin-10 in alveolar macrophages.
Allergy. 56: 1087-90. -
Guntermann, C. and Alexander, D.R. (2002) CTLA-4 suppresses proximal TCR signaling in resting human CD4(+) T cells by inhibiting ZAP-70 Tyr(319) phosphorylation: a potential role for tyrosine phosphatases.
J Immunol. 168: 4420-9. -
Baturcam, E. et al. (2014) Physical exercise reduces the expression of RANTES and its CCR5 receptor in the adipose tissue of obese humans.
Mediators Inflamm. 2014: 627150. -
Clark, E.A. et al. (1983) Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens.
Immunogenetics. 18 (6): 599-615. -
Erber, W.N. et al. (1984) Immunocytochemical detection of T and B cell populations in routine blood smears.
Lancet. 1 (8385): 1042-6. -
Maggiorella, M. et al. (1998) Detection of Infectious Simian Immunodeficiency Virus in B- and T- cell lymphomas of experimentally infected macaques.
Blood. 91 (9): 3103 - 3111. -
Kumpel, B. et al. (2014) Accurate quantitation of D+ fetomaternal hemorrhage by flow cytometry using a novel reagent to eliminate granulocytes from analysis.
Transfusion. 54 (5): 1305-16. -
Mjösberg, J. et al. (2009) Systemic reduction of functionally suppressive CD4dimCD25highFoxp3+ Tregs in human second trimester pregnancy is induced by progesterone and 17beta-estradiol.
J Immunol. 183: 759-69. -
Hess, C. et al. (2000) Induction of neutrophil responsiveness to myeloperoxidase antibodies by their exposure to supernatant of degranulated autologous neutrophils.
Blood. 96: 2822-7. -
Choudhuri, K. et al. (2009) Peptide-major histocompatibility complex dimensions control proximal kinase-phosphatase balance during T cell activation.
J Biol Chem. 284: 26096-105. -
Dong, D. et al. (2006) T cell receptor for antigen induces linker for activation of T cell-dependent activation of a negative signaling complex involving Dok-2, SHIP-1, and Grb-2.
J Exp Med. 203: 2509-18. -
Libri, V. et al. (2008) Jakmip1 is expressed upon T cell differentiation and has an inhibitory function in cytotoxic T lymphocytes.
J Immunol. 181: 5847-56. -
Churchman, S.M. et al. (2014) Modulation of peripheral T-cell function by interleukin-7 in rheumatoid arthritis.
Arthritis Res Ther. 16 (6): 511. -
Ward, S.T. et al. (2015) The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer.
Br J Cancer. 112 (2): 319-28. -
Saavedra, D. et al. (2015) Biomarkers related to immunosenescence: relationships with therapy and survival in lung cancer patients.
Cancer Immunol Immunother. Nov 20. [Epub ahead of print] -
Bhat, S.S. et al. (2016) Syntaxin 8 is required for efficient lytic granule trafficking in cytotoxic T lymphocytes.
Biochim Biophys Acta. Apr 17. pii: S0167-4889(16)30103-3. [Epub ahead of print] -
Hasib, L. et al. (2016) Functional and homeostatic defects of regulatory T cells in patients with coronary artery disease
Journal of Internal Medicine. 279 (1): 63-77. -
Siska, E.K. et al. (2017) Generation of an immortalized mesenchymal stem cell line producing a secreted biosensor protein for glucose monitoring.
PLoS One. 12 (9): e0185498.
Further Reading
-
Clevers, H. et al. (1988) The T cell receptor/CD3 complex: a dynamic protein ensemble.
Annu Rev Immunol. 6: 629-62. -
Arnett, K.L. et al. (2004) Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.
Proc Natl Acad Sci U S A. 101: 16268-73.
