CD69 antibody | FN50
Mouse anti Human CD69:StarBright UltraViolet 665
- Product Type
- Monoclonal Antibody
- Clone
- FN50
- Isotype
- IgG1
- Specificity
- CD69
Filter by Application:
F Reset| Mouse anti Human CD69 antibody, clone FN50 recognizes the human early activation antigen CD69, also known as activation inducer molecule (AIM), Early T-cell activation antigen p60, EA1 or MLR-3. CD69 is a 199 amino acid single pass type II transmembrane glycoprotein of ~30 kDa containing a single C-type lectin domain and a single potential N-glycosylation site. CD69 is expressed as a disulphide bond linked homodimer of ~60 kDa (López-Cabrera et al. 1993). CD69 is a marker of early activation expressed by B and T lymphocytes, natural killer cells(Werfel Mouse anti Human CD69 , clone FN50 is useful for the detection of CD69 by flow cytometry and immunohistochemistry on frozen tissue sections. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Baboon Chimpanzee Cynomolgus monkey Rhesus Monkey Macaque - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright UltraViolet 665 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- Activated human B-cells.
- Approx. Protein Concentrations
- For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright UltraViolet 665 340 669 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | Neat |
- 1Expression of CD69 may be induced in vitro through pre-treatment of cells with phytohemagglutinin (PHA), lipopolysaccharide (LPS), or Phorbol 12-myristate 13-acetate (PMA) with Ionomycin.
Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
References for CD69 antibody
-
Holte, H. et al. (1989) Ki67 and 4F2 antigen expression as well as DNA synthesis predict survival at relapse/tumour progression in low-grade B-cell lymphoma.
Int J Cancer. 44 (6): 975-80. -
Herberth, M. et al. (2010) Differential effects on T-cell function following exposure to serum from schizophrenia smokers.
Mol Psychiatry. 15 (4): 364-71. -
Schaeuble, K. et al. (2011) Cross-talk between TCR and CCR7 signaling sets a temporal threshold for enhanced T lymphocyte migration.
J Immunol. 187 (11): 5645-52. -
Sela, M. et al. (2011) Sequential phosphorylation of SLP-76 at tyrosine 173 is required for activation of T and mast cells.
EMBO J. 30 (15): 3160-72. -
Garbe, Y. et al. (2011) Semiallogenic fusions of MSI(+) tumor cells and activated B cells induce MSI-specific T cell responses.
BMC Cancer. 11: 410. -
Schwitalle, Y. et al. (2004) Immunogenic peptides generated by frameshift mutations in DNA mismatch repair-deficient cancer cells.
Cancer Immun. 4: 14. -
Sutavani, R.V. et al. (2013) CD55 Costimulation Induces Differentiation of a Discrete T Regulatory Type 1 Cell Population with a Stable Phenotype.
J Immunol. 191: 5895-903. -
Walter, G.J. et al. (2013) Interaction with activated monocytes enhances cytokine expression and suppressive activity of human CD4+CD45ro+CD25+CD127(low) regulatory T cells.
Arthritis Rheum. 65: 627-38.
View The Latest Product References
-
Kuric, E. et al. (2017) Demonstration of Tissue Resident Memory CD8 T Cells in Insulitic Lesions in Adult Patients with Recent-Onset Type 1 Diabetes.
Am J Pathol. 187 (3): 581-8. -
Karnell, F.G. et al. (2017) Reconstitution of immune cell populations in multiple sclerosis patients after autologous stem cell transplantation.
Clin Exp Immunol. 189 (3): 268-278. -
Rossatti, P. et al. (2022) Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.
BMC Biol. 20 (1): 189.
- Synonyms
- AIM
- UniProt
- Q07108
- Entrez Gene
- CD69
- GO Terms
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
MCA2806SBUV665
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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