CD29 antibody | 4B7R

Mouse anti Human CD29

Product Type
Monoclonal Antibody
Clone
4B7R
Isotype
IgG1
Specificity
CD29

Product Code Applications Pack Size List Price Your Price Qty
MCA1949GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF IP P * 0.1 mg loader
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loader
MCA1949
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF IP P * 0.2 mg loader
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loader
MCA1949T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF IP P * 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD29 monoclonal antibody, clone 4B7R recognizes the human integrin beta 1 subunit, also known as CD29, a ~130 kDa (red) 115 kDa (non-red) single pass type I transmembrane glycoprotein expressed by most leucocytes and mesenchymal stem cells.

Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Surface expression of CD29 on human natural killer cells can be reduced by pretreatment with the glutathione-S-transferase inhibitor diethyl maleate (Horvath-Arcidiacono et al. 2003)

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA1949GA, MCA1949: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1949T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Occular melanoma cell line V+B2.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Immunofluorescence
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD29 antibody

  1. Marshall, J.F. et al. (1998) Comparative analysis of integrins in vitro and in vivo in uveal and cutaneous melanomas.
    Br J Cancer. 77 (4): 522-9.
  2. Pillay, J. et al. (2010) Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia.
    J Leukoc Biol. 88: 211-20.
  3. Kim, B.S. et al. (2011) Effects of the dichloromethane fraction of dipsaci radix on the osteoblastic differentiation of human alveolar bone marrow-derived mesenchymal stem cells.
    Biosci Biotechnol Biochem. 75:13-9.
  4. Kato, H. et al. (2012) The primacy of β1 integrin activation in the metastatic cascade.
    PLoS One. 7 (10): e46576.
  5. Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
    PLoS One. 6(3):e17454.
  6. Horvath-Arcidiacono, J.A. et al. (2003) Human natural killer cell activity against porcine targets: modulation by control of the oxidation-reduction environment and role of adhesion molecule interactions.
    Cell Immunol. 222: 35-44.
  7. Tan, D.W. et al. (2013) Single-cell gene expression profiling reveals functional heterogeneity of undifferentiated human epidermal cells.
    Development. 140 (7): 1433-44.
  8. Yang, J. et al. (2015) Cordycepin protected against the TNF-α-induced inhibition of osteogenic differentiation of human adipose-derived mesenchymal stem cells.
    Int J Immunopathol Pharmacol. 28 (3): 296-307.
  9. View The Latest Product References
  10. Gu, Q. et al. (2015) Ginkgo biloba extract promotes osteogenic differentiation of human bone marrow mesenchymal stem cells in a pathway involving Wnt/β-catenin signaling.
    Pharmacol Res. 97: 70-8.
  11. Chen, Y. et al. (2015) Effect of human umbilical cord mesenchymal stem cells transplantation on nerve fibers of a rat model of endometriosis.
    Int J Fertil Steril. 9 (1): 71-80.
  12. Lee, H.J. et al. (2017) ICOSL expression in human bone marrow-derived mesenchymal stem cells promotes induction of regulatory T cells.
    Sci Rep. 7: 44486.
  13. Yi, T. et al. (2015) Manufacture of Clinical-Grade Human Clonal Mesenchymal Stem Cell Products from Single Colony Forming Unit-Derived Colonies Based on the Subfractionation Culturing Method.
    Tissue Eng Part C Methods. 21 (12): 1251-62.
  14. Sun, Y. et al. (2017) Antinociceptive Effect of Intrathecal Injection of Genetically Engineered Human Bone Marrow Stem Cells Expressing the Human Proenkephalin Gene in a Rat Model of Bone Cancer Pain.
    Pain Res Manag. 2017: 7346103.
  15. Kim, S.H. et al. (2019) Forkhead box O1 (FOXO1) controls the migratory response of Toll-like receptor (TLR3)-stimulated human mesenchymal stromal cells.
    J Biol Chem. 294 (21): 8424-37.

Synonyms
Integrin Beta 1 Chain
RRID
AB_323203
UniProt
P05556
Entrez Gene
ITGB1
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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