CD29 antibody | 4B7R
Mouse anti Human CD29 antibody, clone 4B7R (MCA1949) used for the demonstration of total β1 integrin expression in formalin-fixed paraffin-embedded tumor cell specimens by immunofluorescence.
Image caption:
Activated β11 integrin in formalin-fixed paraffin-embedded tumor cell specimens. 106 β1-WT cells, β1 integrin knockdown cells (β1 shRNA) and constitutively-active β1-L358A MDA-MB435 cells were resuspended in Matrigel and implanted onto the chorioallantoic membrane of day 10 chick embryos. After seven days of additional incubation, tumors were fixed in formalin and embedded into paraffin. Tumor sections were incubated with proteinase K for antigen retrieval and stained with antibodies for total β1 integrin (4B7R) and activated β1 integrin (9EG7).
From: Kato H, Liao Z, Mitsios JV, Wang H-Y, Deryugina EI, Varner JA, et al. (2012)
The Primacy of β1 Integrin Activation in the Metastatic Cascade.
PLoS ONE 7(10): e46576.
Mouse anti Human CD29 antibody, clone 4B7R (MCA1949) used for the demonstration of total β1 integrin expression in formalin-fixed paraffin-embedded tumor cell specimens by immunofluorescence.
Image caption:
Activated β1 integrins in primary and metastatic human tumors. (A and B) Representative immunostaining of FFPE tissue sections for total β1 integrin (antibody 4B7R) and activated β1 integrin (antibody 9EG7) in primary and metastatic tumors. For detection and analysis of tumor cells, samples were stained with an antibody to pan-keratin (breast cancer) (A) or MART-1 (melanoma) (B). The breast cancer metastasis was from a lymph node and the melanoma metastasis was from the abdominal cavity. Primary and metastatic tumors were from different patients. Note that in the primary breast tumor and metastatic melanoma, activated β1 integrins were observed in some connective tissue cells as well as in tumor cells. Fluorescence images of primary and metastatic tumors were processed identically. (C) Box plot showing β1 integrin activation in primary (n = 50) and metastatic (n = 26) melanomas in a tissue microarray. The proportion of areas with activated β1 integrin to total β1 integrin within MART-1 positive melanoma cells was analyzed with Volocity software. Statistical analysis was performed by Mann-Whitney U test (Outliers, values outside of 1.5 X interquartile distance from the quartiles, are indicated by an X).
From: Kato H, Liao Z, Mitsios JV, Wang H-Y, Deryugina EI, Varner JA, et al. (2012)
The Primacy of β1 Integrin Activation in the Metastatic Cascade.
PLoS ONE 7(10): e46576.
Mouse anti Human CD29 antibody, clone 4B7R (MCA1949) used for the evaluation of integrin β1 expression on human muscle derived cells by flow cytometry and immunofluorescence microscopy.
Image caption:
Figure 1. Characterization and phenotype of human mdcs. a. Cultured human mdcs consisted of small, triangular adherent cells and small, round floating cells. Scale bar = 50 μm. b. Proliferation curve of one normal human mdc preparation (pN1) and one DMD mdc preparation (pD1). The average mean population doubling time for these cells is 33.79±1.24 hrs. c, d, e, f. Representative images showing the expression of NG2 (c, green), CD49b (d, green), CD29 (e, green) and CD44 (f, green) by pN1 human mdcs. Nuclei were counterstained with 10 µg/ml DAPI (blue). Scale bar = 50 μm. g. Representative FACS images showing the expression of PDGFRβ, ALP, CD49b, CD29, CD71, CD90, Stro-1, CD56 and CD34 by human mdcs. Isotype control is shown in red in each image, and positive signal is shown in green.
From: Meng J, Adkin CF, Xu S-w, Muntoni F, Morgan JE (2011)
Contribution of Human Muscle-Derived Cells to Skeletal Muscle Regeneration in Dystrophic Host Mice.
PLoS ONE 6(3): e17454.
Mouse anti Human CD29 antibody, clone 4B7R (MCA1949GA) used for the identification of mesenchymal stem cells in mixed bonemarrow cell populations by flow cytometry.
Image caption:
MSCs used in this study were characterized for marker expression by flow cytometric analysis. Dashed histograms indicate staining with isotype-matched control antibody and solid histograms denote the specific expression of each indicated marker.
From: Lee HJ, Kim SN, Jeon MS, Yi T, Song SU.
ICOSL expression in human bone marrow-derived mesenchymal stem cells promotes induction of regulatory T cells.
Sci Rep. 2017 Mar 14;7:44486.
Mouse anti Human CD29:Alexa Fluor® 647
Mouse anti Human CD29:FITC
Mouse anti Human CD29
Mouse anti Human CD29:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 4B7R
- Isotype
- IgG1
Mouse anti Human CD29 monoclonal antibody, clone 4B7R recognizes the human integrin beta 1 subunit, also known as CD29, a ~130 kDa (red) 115 kDa (non-red) single pass type I transmembrane glycoprotein expressed by most leucocytes and mesenchymal stem cells.
Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Surface expression of CD29 on human natural killer cells can be reduced by pretreatment with the glutathione-S-transferase inhibitor diethyl maleate (Horvath-Arcidiacono et al. 2003)
Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Surface expression of CD29 on human natural killer cells can be reduced by pretreatment with the glutathione-S-transferase inhibitor diethyl maleate (Horvath-Arcidiacono et al. 2003)
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - conjugated to Alexa Fluor®647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Pack Size: 25 TestsReconstitute in 0.25 ml disilled waterPack Size: 100 TestsReconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Occular melanoma cell line V+B2.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P05556 Related reagents
- Entrez Gene
- ITGB1 Related reagents
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD29 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | Neat Pack Size: 25 µg 1/10 Pack Size: 0.1 mg |
|
| Flow Cytometry | 1/25 | 1/50 | |
| Immunofluorescence | |||
| Immunohistology - Paraffin 1 | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | Neat | 1/2 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | 100 Tests/1ml | F | ||
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F | ||
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F | ||
| Mouse IgG1 Negative Control:RPE | MCA928PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for CD29 antibody
-
Marshall, J.F. et al. (1998) Comparative analysis of integrins in vitro and in vivo in uveal and cutaneous melanomas.
Br J Cancer. 77 (4): 522-9. -
Pillay, J. et al. (2010) Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia.
J Leukoc Biol. 88: 211-20. -
Kim, B.S. et al. (2011) Effects of the dichloromethane fraction of dipsaci radix on the osteoblastic differentiation of human alveolar bone marrow-derived mesenchymal stem cells.
Biosci Biotechnol Biochem. 75:13-9. -
Kato, H. et al. (2012) The primacy of β1 integrin activation in the metastatic cascade.
PLoS One. 7 (10): e46576. -
Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
PLoS One. 6(3):e17454. -
Horvath-Arcidiacono, J.A. et al. (2003) Human natural killer cell activity against porcine targets: modulation by control of the oxidation-reduction environment and role of adhesion molecule interactions.
Cell Immunol. 222: 35-44. -
Tan, D.W. et al. (2013) Single-cell gene expression profiling reveals functional heterogeneity of undifferentiated human epidermal cells.
Development. 140 (7): 1433-44. -
Yang, J. et al. (2015) Cordycepin protected against the TNF-α-induced inhibition of osteogenic differentiation of human adipose-derived mesenchymal stem cells.
Int J Immunopathol Pharmacol. 28 (3): 296-307. -
Gu, Q. et al. (2015) Ginkgo biloba extract promotes osteogenic differentiation of human bone marrow mesenchymal stem cells in a pathway involving Wnt/β-catenin signaling.
Pharmacol Res. 97: 70-8. -
Chen, Y. et al. (2015) Effect of human umbilical cord mesenchymal stem cells transplantation on nerve fibers of a rat model of endometriosis.
Int J Fertil Steril. 9 (1): 71-80. -
Lee, H.J. et al. (2017) ICOSL expression in human bone marrow-derived mesenchymal stem cells promotes induction of regulatory T cells.
Sci Rep. 7: 44486. -
Yi, T. et al. (2015) Manufacture of Clinical-Grade Human Clonal Mesenchymal Stem Cell Products from Single Colony Forming Unit-Derived Colonies Based on the Subfractionation Culturing Method.
Tissue Eng Part C Methods. 21 (12): 1251-62. -
Sun, Y. et al. (2017) Antinociceptive Effect of Intrathecal Injection of Genetically Engineered Human Bone Marrow Stem Cells Expressing the Human Proenkephalin Gene in a Rat Model of Bone Cancer Pain.
Pain Res Manag. 2017: 7346103.
