CD257 antibody | A01-1A12
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Mouse anti Human CD257
- Product Type
- Monoclonal Antibody
- Clone
- A01-1A12
- Isotype
- IgG2a
- Specificity
- CD257
Antibody Quality and 
Unrivaled 
| Mouse anti Human CD257 antibody, clone A01-1A12 recognizes CD257, a ~31 kDa protein. CD257 is also known as B cell activating factor (BAFF), tumor necrosis factor superfamily 13B (TNFSF13B), B lymphocyte stimulator (BLyS) and TNF and APOL-related leukocyte expressed ligand. CD257 is a type 2 transmembrane protein which can be cleaved proteolyically to form a soluble protein. CD257 exerts anti-apoptotic effects and promotes B cell survival via CD267 (TACI), CD269 (BCMA) and CD268 (BAFFR) receptors, the latter interaction playing a critical role in the activation, survival, differentiation and proliferation of B cells (Mackay & Schneider, 2009). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- E. coli derived recombinant protein corresponding to aa 134-285 of human CD257
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fuzed with cells of the mouse SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Further Reading
-
Mackay, F. & Schneider, P. (2009) Cracking the BAFF code.
Nat Rev Immunol. 9 (7): 491-502.
- Synonyms
- BAFF
- BLyS
- UniProt
- Q9Y275
- Entrez Gene
- TNFSF13B
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0007165 signal transduction
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0005625 soluble fraction
- GO:0006955 immune response
- GO:0008283 cell proliferation
- View More GO Terms
View more products with CD257 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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