CD257 antibody | 2B
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Mouse anti Human CD257
- Product Type
- Monoclonal Antibody
- Clone
- 2B
- Isotype
- IgG1
- Specificity
- CD257
Antibody Quality and Performance Guarantee
| Mouse anti Human CD257 antibody, clone 2B recognises CD257, also known as BAFF/BLyS. CD257 is a 285 amino acid type II transmembrane protein and member of the tumor necrosis factor (TNF) superfamily of ligands, which acts as a B and T cell stimulator and plays a role in the development of autoimmune diseases such as systemic lupus erythematosus. CD257 is expressed by monocytes, macrophages, T cells and dendritic cells and acts through binding to CD267 (TACI), CD269 (BCMA) and CD268 (BAFFR) receptors, the latter interaction playing a critical role in the activation, survival, differentiation and proliferation of B cells. Mouse anti Human CD257 antibody, clone 2B has not been cross-adsorbed and may react with homologous proteins from related species. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.5ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant 17 kDa polypeptide corresponding to the TNF-like portion of the extracellular domain of human CD257, expressed in E. coli.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5 | 6ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Further Reading
-
Bossen, C. & Schneider, P. (2006) BAFF, APRIL and their receptors: structure, function and signaling.
Semin Immunol. 18 (5): 263-75.
- Synonyms
- BAFF
- BLyS
- RRID
- AB_2206823
- UniProt
- Q9Y275
- Entrez Gene
- TNFSF13B
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0007165 signal transduction
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0005625 soluble fraction
- GO:0006955 immune response
- GO:0008283 cell proliferation
- View More GO Terms
MCA2677
149165
164852
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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