CD257 antibody | 2B

Mouse anti Human CD257

Product Type
Monoclonal Antibody
Clone
2B
Isotype
IgG1
Specificity
CD257

Product Code Applications Pack Size List Price Your Price Qty
MCA2677
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.5 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD257 antibody, clone 2B recognises CD257, also known as BAFF/BLyS. CD257 is a 285 amino acid type II transmembrane protein and member of the tumor necrosis factor (TNF) superfamily of ligands, which acts as a B and T cell stimulator and plays a role in the development of autoimmune diseases such as systemic lupus erythematosus.

CD257 is expressed by monocytes, macrophages, T cells and dendritic cells and acts through binding to CD267 (TACI), CD269 (BCMA) and CD268 (BAFFR) receptors, the latter interaction playing a critical role in the activation, survival, differentiation and proliferation of B cells. Mouse anti Human CD257 antibody, clone 2B has not been cross-adsorbed and may react with homologous proteins from related species.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.5ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant 17 kDa polypeptide corresponding to the TNF-like portion of the extracellular domain of human CD257, expressed in E. coli.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5 6ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Further Reading

  1. Bossen, C. & Schneider, P. (2006) BAFF, APRIL and their receptors: structure, function and signaling.
    Semin Immunol. 18 (5): 263-75.

Synonyms
BAFF
BLyS
RRID
AB_2206823
UniProt
Q9Y275
Entrez Gene
TNFSF13B
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0007165 signal transduction
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006955 immune response
GO:0008283 cell proliferation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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