CD238 antibody | BRIC203
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Mouse anti Human CD238 antibody, clone BRIC203 recognizes human CD238, also known as the kell blood group antigen Kpbc. The kell blood group antigens are carried on the ~93 kDa erythrocyte membrane glycoprotein.
In hemagglutination tests, Mouse anti Human CD238 antibody, clone BRIC203 reacts with Kp (a-b+) but not Kp (a+b-). In addition Mouse anti Human CD238 antibody, clone BRIC203 will agglutinate Kp (a+b-c+) and Kp (a-b-c+) erythrocytes but not react with Ko erythrocytes.
Weaker staining in flow cytometry is observed on erythroblasts derived from CD34+ hematopoietic progenitor cells, compared to Kp (a-b+) red blood cells.
In hemagglutination tests, Mouse anti Human CD238 antibody, clone BRIC203 reacts with Kp (a-b+) but not Kp (a+b-). In addition Mouse anti Human CD238 antibody, clone BRIC203 will agglutinate Kp (a+b-c+) and Kp (a-b-c+) erythrocytes but not react with Ko erythrocytes.
Weaker staining in flow cytometry is observed on erythroblasts derived from CD34+ hematopoietic progenitor cells, compared to Kp (a-b+) red blood cells.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
≤100mM Glycine - Immunogen
- Human erythrocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c were fused with cells of a myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P23276
- Entrez Gene
- KEL
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0004222 metalloendopeptidase activity
- GO:0016021 integral to membrane
- GO:0046872 metal ion binding
- GO:0006508 proteolysis
- GO:0042310 vasoconstriction
- Regulatory
- For research purposes only
Applications of CD238 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Haemagglutination | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for CD238 antibody
-
Parsons, S.F. et al. (1993) Monoclonal antibodies against Kell glycoprotein: serology, immunochemistry and quantification of antigen sites.
Transfus Med. 3 (2): 137-42. -
Van Der Schoot. et al. (2002) CD238 - Kell.
Leucocyte Typing VII pp 584-5. -
Miyazak, T. et al. (2002) Serological analyses of anti-kell monoclonal antibodies using MAIEA assay.
Leucocyte Typing VII pp 585-8.
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