CD238 antibody | BRIC203

Mouse anti Human CD238

Product Type
Monoclonal Antibody
Clone
BRIC203
Isotype
IgG1
Specificity
CD238

Product Code Applications Pack Size List Price Your Price Qty
MCA1987
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SDS Safety Datasheet SDS
F H IP 0.2 mg loader
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loader
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Mouse anti Human CD238 antibody, clone BRIC203 recognizes human CD238, also known as the kell blood group antigen Kpbc. The kell blood group antigens are carried on the ~93 kDa erythrocyte membrane glycoprotein.

In hemagglutination tests, Mouse anti Human CD238 antibody, clone BRIC203 reacts with Kp (a-b+) but not Kp (a+b-). In addition Mouse anti Human CD238 antibody, clone BRIC203 will agglutinate Kp (a+b-c+) and Kp (a-b-c+) erythrocytes but not react with Ko erythrocytes.

Weaker staining in flow cytometry is observed on erythroblasts derived from CD34+ hematopoietic progenitor cells, compared to Kp (a-b+) red blood cells.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
≤100mM Glycine
Immunogen
Human erythrocytes.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c were fused with cells of a myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Haemagglutination
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for CD238 antibody

  1. Parsons, S.F. et al. (1993) Monoclonal antibodies against Kell glycoprotein: serology, immunochemistry and quantification of antigen sites.
    Transfus Med. 3 (2): 137-42.
  2. Van Der Schoot. et al. (2002) CD238 - Kell.
    Leucocyte Typing VII pp 584-5.
  3. Miyazak, T. et al. (2002) Serological analyses of anti-kell monoclonal antibodies using MAIEA assay.
    Leucocyte Typing VII pp 585-8.

RRID
AB_2132640
UniProt
P23276
Entrez Gene
KEL
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0004222 metalloendopeptidase activity
GO:0016021 integral to membrane
GO:0046872 metal ion binding
GO:0006508 proteolysis
GO:0042310 vasoconstriction
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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