CD227 antibody | VU-4H5


Filter by Application:
E ResetMouse anti Human CD227
- Product Type
- Monoclonal Antibody
- Clone
- VU-4H5
- Isotype
- IgG1
- Specificity
- CD227
Mouse anti Human CD227 antibody, clone VU-4H5 recognizes human Mucin 1, also known as CD227, Cancer antigen 15-3, Episialin or Krebs von den Lungen-6. CD227 is a 1255 amino acid single pass type I transmembrane glycoprotein. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is a breast cancer associated mucin encoded by the Muc-1 gene. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases. Mouse anti Human CD227 antibody, clone VU-4H5 reacts with the APDTR peptide sequence (VTSAPDTRPAPGSTAPPAHG) of human CD227. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- 60-mer tandem repeat of VTSAPDTRPAPGSTAPPAHG, conjugated to BSA.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
||
Immunohistology - Frozen | ![]() |
||
Immunohistology - Paraffin | ![]() |
||
Western Blotting | ![]() |
References for CD227 antibody
-
Treon, S.P. et al. (1999) Muc-1 core protein is expressed on multiple myeloma cells and is induced by dexamethasone.
Blood. 93: 1287-98. -
Karsten, U. et al. (2004) Binding patterns of DTR-specific antibodies reveal a glycosylation-conditioned tumor-specific epitope of the epithelial mucin (MUC1).
Glycobiology. 14: 681-92. -
Treon, S.P. et al. (2000) Elevated soluble MUC1 levels and decreased anti-MUC1 antibody levels in patients with multiple myeloma.
Blood. 96: 3147-53. -
Singh, R. et al. (2007) Target-specific cytotoxic activity of recombinant immunotoxin scFv(MUC1)-ETA on breast carcinoma cells and primary breast tumors.
Mol Cancer Ther. 6: 562-9. -
Vlad, A.M. et al. (2002) Complex carbohydrates are not removed during processing of glycoproteins by dendritic cells: processing of tumor antigen MUC1 glycopeptides for presentation to major histocompatibility complex class II-restricted T cells.
J Exp Med. 196: 1435-46. -
ten Berge, R.L. et al. (2001) MUC1 (EMA) is preferentially expressed by ALK positive anaplastic large cell lymphoma, in the normally glycosylated or only partly hypoglycosylated form.
J Clin Pathol. 54: 933-9.
1695-0028


If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up