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CD227 antibody | VU-3C6

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Anti Human CD227 Antibody, clone VU-3C6 thumbnail image 1 Anti Human CD227 Antibody, clone VU-3C6 thumbnail image 2
Anti Human CD227 Antibody, clone VU-3C6 gallery image 1
Western Blot analysis of MCF7 whole cell lysate probed with Mouse anti human CD227 (6378-0135) followed by Rabbit F(ab')2 anti Mouse IgG:HRP (STAR13B) performed under reducing conditions.
Anti Human CD227 Antibody, clone VU-3C6 gallery image 2
Published customer image:
Mouse anti Human CD227 antibody, clone VU-3C6 (6378-0135) used to identify Muc-1 expression in human breast cancer tissue by immunohistochemistry.
Image caption:
Control VU3C6. C, positive control for VU3C6. Breast cancer tissue.

From: Engelstaedter V, Heublein S, Schumacher AL, Lenhard M, Engelstaedter H, Andergassen U, Guenthner-Biller M, Kuhn C, Rack B, Kupka M, Mayr D, Jeschke U.
Mucin-1 and its relation to grade, stage and survival in ovarian carcinoma patients.
BMC Cancer. 2012 Dec 15;12:600.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD227

Product Type
Monoclonal Antibody
Clone
VU-3C6
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
6378-0135
Datasheet
C F IP P* WB
MSDS
0.2 mg loader

Mouse anti Human CD227 antibody, clone VU-3C6 recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.

The dominant epitope recognized by this antibody is the 12-mer GVTSAPDTRPAP of the mucin 1 tandem repeat.

Mouse anti Human CD227 antibody has been tested on frozen human ovarian carcinoma tissue sections and paraffin embedded sections from normal breast tissue. Mouse anti Human CD227 antibody, clone VU-3C6 also reacts weakly with sections of small intestine and colon.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Human breast cancer cell line ZR-75-1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P15941
Entrez Gene
MUC1
Regulatory
For research purposes only

Applications of CD227 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting 1/200 1/1000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Flow Cytometry

Immunoprecipitation

Product Specific References

References for CD227 antibody

  1. Meng, C.X. et al. (2010) Effects of oral and vaginal administration of levonorgestrel emergency contraception on markers of endometrial receptivity.
    Hum Reprod. 25 (4): 874-83.
  2. Treon, S.P. et al. (1999) Muc-1 core protein is expressed on multiple myeloma cells and is induced by dexamethasone.
    Blood. 93: 1287-98.
  3. Treon, S.P. et al. (2000) Elevated soluble MUC1 levels and decreased anti-MUC1 antibody levels in patients with multiple myeloma.
    Blood. 96: 3147-53.
  4. Karsten, U. et al. (2004) Binding patterns of DTR-specific antibodies reveal a glycosylation-conditioned tumor-specific epitope of the epithelial mucin (MUC1).
    Glycobiology. 14: 681-92.
  5. Kinlough, C.L. et al. (2006) Recycling of MUC1 is dependent on its palmitoylation.
    J Biol Chem. 281: 12112-22.
  6. Vlad, A.M. et al. (2002) Complex carbohydrates are not removed during processing of glycoproteins by dendritic cells: processing of tumor antigen MUC1 glycopeptides for presentation to major histocompatibility complex class II-restricted T cells.
    J Exp Med. 196: 1435-46.
  7. Danielczyk, A. et al. (2006) PankoMab: a potent new generation anti-tumour MUC1 antibody.
    Cancer Immunol Immunother. 55 (11): 1337-47.
  8. Engelstaedter, V. et al. (2012) Mucin-1 and its relation to grade, stage and survival in ovarian carcinoma patients.
    BMC Cancer. 12: 600.
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