CD227 antibody | VU-3C6
Mouse anti Human CD227 antibody, clone VU-3C6 (6378-0135) used to identify Muc-1 expression in human breast cancer tissue by immunohistochemistry.
Image caption:
Control VU3C6. C, positive control for VU3C6. Breast cancer tissue.
From: Engelstaedter V, Heublein S, Schumacher AL, Lenhard M, Engelstaedter H, Andergassen U, Guenthner-Biller M, Kuhn C, Rack B, Kupka M, Mayr D, Jeschke U.
Mucin-1 and its relation to grade, stage and survival in ovarian carcinoma patients.
BMC Cancer. 2012 Dec 15;12:600.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD227 antibody, clone VU-3C6 recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.
The dominant epitope recognized by this antibody is the 12-mer GVTSAPDTRPAP of the mucin 1 tandem repeat.
Mouse anti Human CD227 antibody has been tested on frozen human ovarian carcinoma tissue sections and paraffin embedded sections from normal breast tissue. Mouse anti Human CD227 antibody, clone VU-3C6 also reacts weakly with sections of small intestine and colon.
The dominant epitope recognized by this antibody is the 12-mer GVTSAPDTRPAP of the mucin 1 tandem repeat.
Mouse anti Human CD227 antibody has been tested on frozen human ovarian carcinoma tissue sections and paraffin embedded sections from normal breast tissue. Mouse anti Human CD227 antibody, clone VU-3C6 also reacts weakly with sections of small intestine and colon.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human breast cancer cell line ZR-75-1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
Applications of CD227 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Immunoprecipitation | |||
| Western Blotting | 1/200 | 1/1000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for CD227 antibody
-
Meng, C.X. et al. (2010) Effects of oral and vaginal administration of levonorgestrel emergency contraception on markers of endometrial receptivity.
Hum Reprod. 25 (4): 874-83. -
Treon, S.P. et al. (1999) Muc-1 core protein is expressed on multiple myeloma cells and is induced by dexamethasone.
Blood. 93: 1287-98. -
Treon, S.P. et al. (2000) Elevated soluble MUC1 levels and decreased anti-MUC1 antibody levels in patients with multiple myeloma.
Blood. 96: 3147-53. -
Karsten, U. et al. (2004) Binding patterns of DTR-specific antibodies reveal a glycosylation-conditioned tumor-specific epitope of the epithelial mucin (MUC1).
Glycobiology. 14: 681-92. -
Kinlough, C.L. et al. (2006) Recycling of MUC1 is dependent on its palmitoylation.
J Biol Chem. 281: 12112-22. -
Vlad, A.M. et al. (2002) Complex carbohydrates are not removed during processing of glycoproteins by dendritic cells: processing of tumor antigen MUC1 glycopeptides for presentation to major histocompatibility complex class II-restricted T cells.
J Exp Med. 196: 1435-46. -
Danielczyk, A. et al. (2006) PankoMab: a potent new generation anti-tumour MUC1 antibody.
Cancer Immunol Immunother. 55 (11): 1337-47. -
Engelstaedter, V. et al. (2012) Mucin-1 and its relation to grade, stage and survival in ovarian carcinoma patients.
BMC Cancer. 12: 600.
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