CD227 antibody | VU-3C6

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Anti Human CD227 Antibody, clone VU-3C6 thumbnail image 1

Anti Human CD227 Antibody, clone VU-3C6 thumbnail image 2

Anti Human CD227 Antibody, clone VU-3C6 gallery image 1 — Western Blot analysis of MCF7 whole cell lysate probed with Mouse anti human CD227 (**6378-0135**) followed by Rabbit F(ab')₂ anti Mouse IgG:HRP (STAR13B) performed under reducing conditions.

Anti Human CD227 Antibody, clone VU-3C6 gallery image 2 — **Published customer image:**
Mouse anti Human CD227 antibody, clone VU-3C6 (**6378-0135**) used to identify Muc-1 expression in human breast cancer tissue by immunohistochemistry.
**Image caption:**
Control VU3C6. C, positive control for VU3C6. Breast cancer tissue.

From: Engelstaedter V, Heublein S, Schumacher AL, Lenhard M, Engelstaedter H, Andergassen U, Guenthner-Biller M, Kuhn C, Rack B, Kupka M, Mayr D, Jeschke U.
Mucin-1 and its relation to grade, stage and survival in ovarian carcinoma patients.
BMC Cancer. 2012 Dec 15;12:600.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD227

Product Type: Monoclonal Antibody
Clone: VU-3C6
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
6378-0135	C F IP P* WB			0.2 mg

Mouse anti Human CD227 antibody, clone VU-3C6 recognizes CD227, also known as mucin 1 which is a breast cancer associated mucin encoded by the Muc-1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.

The dominant epitope recognized by this antibody is the 12-mer GVTSAPDTRPAP of the mucin 1 tandem repeat.

Mouse anti Human CD227 antibody has been tested on frozen human ovarian carcinoma tissue sections and paraffin embedded sections from normal breast tissue. Mouse anti Human CD227 antibody, clone VU-3C6 also reacts weakly with sections of small intestine and colon.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Carrier Free: Yes
Immunogen: Human breast cancer cell line ZR-75-1.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P15941 Related reagents
Entrez Gene: MUC1 Related reagents
Regulatory: For research purposes only

Applications of CD227 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin ¹
Immunoprecipitation
Western Blotting	1/200	1/1000

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Flow Cytometry

Immunoprecipitation

Product Specific References

References for CD227 antibody

Meng, C.X. et al. (2010) Effects of oral and vaginal administration of levonorgestrel emergency contraception on markers of endometrial receptivity.
Hum Reprod. 25 (4): 874-83.
Treon, S.P. et al. (1999) Muc-1 core protein is expressed on multiple myeloma cells and is induced by dexamethasone.
Blood. 93: 1287-98.
Treon, S.P. et al. (2000) Elevated soluble MUC1 levels and decreased anti-MUC1 antibody levels in patients with multiple myeloma.
Blood. 96: 3147-53.
Karsten, U. et al. (2004) Binding patterns of DTR-specific antibodies reveal a glycosylation-conditioned tumor-specific epitope of the epithelial mucin (MUC1).
Glycobiology. 14: 681-92.
Kinlough, C.L. et al. (2006) Recycling of MUC1 is dependent on its palmitoylation.
J Biol Chem. 281: 12112-22.
Vlad, A.M. et al. (2002) Complex carbohydrates are not removed during processing of glycoproteins by dendritic cells: processing of tumor antigen MUC1 glycopeptides for presentation to major histocompatibility complex class II-restricted T cells.
J Exp Med. 196: 1435-46.
Danielczyk, A. et al. (2006) PankoMab: a potent new generation anti-tumour MUC1 antibody.
Cancer Immunol Immunother. 55 (11): 1337-47.
Engelstaedter, V. et al. (2012) Mucin-1 and its relation to grade, stage and survival in ovarian carcinoma patients.
BMC Cancer. 12: 600.

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