CD226 antibody | DX11

Mouse anti Human CD226

Product Type
Monoclonal Antibody
Clone
DX11
Isotype
IgG1
Specificity
CD226

Product Code Applications Pack Size List Price Your Price Qty
MCA2257GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.1 mg loader
List Price Your Price
loader
MCA2257
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD226 antibody, clone DX11 recognizes human CD226, a ~65 kDa glycoprotein, also known as DNAM1 (DNAX accessory molecule-1). CD226 is broadly expressed on T-cells, NK cells, platelets, monocytes and a subset of B cells. CD226 is also expressed by a subset of CD3 positive thymocytes.

Mouse anti Human CD226 antibody, clone DX11 is reported to inhibit T- and NK cell mediated cytotoxicity against tumor cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells (Kojima et al. 2003).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human cytotoxic T lymphocyte clone
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mouse were fused with cells of the Sp2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 Pack Size: 0.1 mg
1/50 Pack Size: 0.2 mg
1/100 Pack Size: 0.1 mg
1/200 Pack Size: 0.2 mg
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.

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Description Mouse IgG1 Negative Control

References for CD226 antibody

  1. Shibuya, A. et al. (1996) DNAM-1, a novel adhesion molecule involved in the cytolytic function of T lymphocytes.
    Immunity. 4 (6): 573-81.
  2. Shibuya, A. et al. (1998) Protein kinase C is involved in the regulation of both signaling and adhesion mediated by DNAX accessory molecule-1 receptor.
    J Immunol. 161 (4): 1671-6.
  3. Kojima, H. et al. (2003) CD226 mediates platelet and megakaryocytic cell adhesion to vascular endothelial cells.
    J Biol Chem. 278 (38): 36748-53.
  4. Manes, T.D. and Pober, J.S. (2011) Identification of Endothelial Cell Junctional Proteins and Lymphocyte Receptors Involved in Transendothelial Migration of Human Effector Memory CD4+ T Cells.
    J Immunol. 186: 1763-8.
  5. Ardolino, M. et al. (2011) DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction.
    Blood. 117: 4778-86.
  6. Soriani, A. et al. (2009) ATM-ATR-dependent up-regulation of DNAM-1 and NKG2D ligands on multiple myeloma cells by therapeutic agents results in enhanced NK-cell susceptibility and is associated with a senescent phenotype.
    Blood. 113: 3503-11.
  7. Fionda, C. et al. (2009) Heat shock protein-90 inhibitors increase MHC class I-related chain A and B ligand expression on multiple myeloma cells and their ability to trigger NK cell degranulation.
    J Immunol. 183 (7): 4385-94.
  8. Matusali, G. et al. (2012) The Human Immunodeficiency Virus Type 1 Nef and Vpu Proteins Downregulate the Natural Killer Cell-Activating Ligand PVR.
    J Virol. 86: 4496-504.
  9. View The Latest Product References
  10. Fionda, C. et al. (2015) Nitric oxide donors increase PVR/CD155 DNAM-1 ligand expression in multiple myeloma cells: role of DNA damage response activation.
    BMC Cancer. 15 (1): 17.
  11. Molfetta, R. et al. (2019) The Ubiquitin-proteasome pathway regulates Nectin2/CD112 expression and impairs NK cell recognition and killing.
    Eur J Immunol. 49 (6): 873-83.
  12. Vulpis, E. et al. (2022) Impact on NK cell functions of acute versus chronic exposure to extracellular vesicle-associated MICA: Dual role in cancer immunosurveillance.
    J Extracell Vesicles. 11 (1): e12176.
  13. Molfetta, R. et al. (2020) CD155: A Multi-Functional Molecule in Tumor Progression.
    Int J Mol Sci. 21 (3): 922.
  14. Zitti, B. et al. (2017) Innate immune activating ligand SUMOylation affects tumor cell recognition by NK cells.
    Sci Rep. 7 (1): 10445.
  15. Mekhloufi, A. et al. (2020) Bone Marrow Stromal Cell-Derived IL-8 Upregulates PVR Expression on Multiple Myeloma Cells via NF-kB Transcription Factor.
    Cancers (Basel). 12 (2): 440.

Synonyms
DNAM-1
RRID
AB_324895
UniProt
Q15762
Entrez Gene
CD226
GO Terms
GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
GO:0002891 positive regulation of immunoglobulin mediated immune response
GO:0007155 cell adhesion
GO:0050839 cell adhesion molecule binding
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005178 integrin binding
GO:0008037 cell recognition
GO:0009986 cell surface
GO:0019901 protein kinase binding
GO:0033005 positive regulation of mast cell activation
GO:0045121 membrane raft
GO:0060369 positive regulation of Fc receptor mediated stimulatory signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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