CD221 antibody | 1H7
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Mouse anti Human CD221
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD221 antibody, clone 1H7 recognizes human CD221, a ~155 kDa receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by western blotting (Li et al.1993).
CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin.
The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signaling proteins. Clone 1H7 is reported to partially block binding of IGF-I and IGF-II to CD221 (Li et al.1993).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Purified human placental IGF-I receptor.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1||1/10||1/40|
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- Histology Positive Control Tissue
- Human pancreas or placenta
References for CD221 antibody
Li, S.L. et al. (1993) Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor.
Biochem Biophys Res Commun. 196 (1): 92-8.
Beauvais, D.M. & Rapraeger, A.C. (2010) Syndecan-1 couples the insulin-like growth factor-1 receptor to inside-out integrin activation.
J Cell Sci. 123 (Pt 21): 3796-807.
Krieger, C.C. et al. (2016) Thyrotropin/IGF-1 receptor crosstalk in Graves' ophthalmopathy pathogenesis.
J Clin Endocrinol Metab. Apr 4: jc20161315. [Epub ahead of print]
van Valen, F. et al. (2012) A Novel Role of IGF1 in Apo2L/TRAIL-Mediated Apoptosis of Ewing Tumor Cells.
Sarcoma. 2012: 782970.
- IGF-1 Receptor
- Entrez Gene
- GO Terms
- GO:0005010 insulin-like growth factor receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005158 insulin receptor binding
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0005792 microsome
- GO:0006916 anti-apoptosis
- GO:0006955 immune response
- GO:0008284 positive regulation of cell proliferation
- View More GO Terms
- GO:0008286 insulin receptor signaling pathway
- GO:0042802 identical protein binding
- GO:0014065 phosphatidylinositol 3-kinase cascade
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0030335 positive regulation of cell migration
- GO:0031994 insulin-like growth factor I binding
- GO:0043548 phosphatidylinositol 3-kinase binding
- GO:0043559 insulin binding
- GO:0043560 insulin receptor substrate binding
- GO:0045740 positive regulation of DNA replication
- GO:0046777 protein autophosphorylation
- GO:0051262 protein tetramerization
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