CD21 antibody | LB21
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Mouse anti Human CD21
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD21 antibody, clone LB21 recognizes the human Complement receptor type 2, also known as CD21 or the Epstein-Barr virus receptor. CD21 is a ~14 kDa cell surface glycoprotein expressed by mature B cells and by follicular dendritic cells. The molecule acts as a receptor for complement components C3d, C3dg and iC3b, as well as for Epstein Barr Virus. It forms part of a large signal transduction complex in association with CD19.|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Sheep Mink Bovine Goat Cat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by ion exchange chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
- Human IM9 cell line.
- Approx. Protein Concentrations
- IgG concentration 25 ug/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1||1/10||1/100|
|Immunohistology - Paraffin|
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl human whole blood
- Histology Positive Control Tissue
- Lymph node
References for CD21 antibody
Furukawa, Y. et al. (2000) Frequent reversible membrane damage in peripheral blood B cells in human T cell lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP).
Clin Exp Immunol. 120 (2): 307-16.
Aichem, A. et al. (2006) Redox regulation of CD21 shedding involves signaling via PKC and indicates the formation of a juxtamembrane stalk.
J Cell Sci. 119: 2892-902.
Sengstake, S. et al. (2006) CD21 and CD62L shedding are both inducible via P2X7Rs.
Int Immunol. 18: 1171-8.
Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
Vet Immunol Immunopathol. 119: 115-22.
Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37.
Sopp, P. et al. (2007) Cross-reactivity of mAbs to human CD antigens with cells from cattle.
Vet Immunol Immunopathol. 119: 106-14.
Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
Vet Immunol Immunopathol. 119: 123-30.
Angel, C.E. et al. (2009) Distinctive localization of antigen-presenting cells in human lymph nodes.
Blood. 113: 1257-67.
View The Latest Product References
Meister, R.K. et al. (2007) Progress in the discovery and definition of monoclonal antibodies for use in feline research.
Vet Immunol Immunopathol. 119: 38-46.
Clutterbuck, E.A. et al. (2012) Pneumococcal conjugate and plain polysaccharide vaccines have divergent effects on antigen-specific B cells.
J Infect Dis. 205: 1408-16.
Damgaard, B.M. et al. (2012) The effects of feed restriction on physical activity, body weight, physiology, haematology and immunology in female mink.
Res Vet Sci. 93: 936-42.
Stachowiak, B. & Weingartl, H.M. (2012) Nipah virus infects specific subsets of porcine peripheral blood mononuclear cells.
PLoS One. 7 (1): e30855.
Pooley, H.B. et al. (2022) Sheep vaccinated against paratuberculosis have increased levels of B cells infiltrating the intestinal tissue.
Vet Immunol Immunopathol. 252: 110482.
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