CD21 antibody | LB21
Mouse anti Human CD21
- Product Type
- Monoclonal Antibody
- Clone
- LB21
- Isotype
- IgG1
- Specificity
- CD21
Antibody Quality and Performance Guarantee
| Mouse anti Human CD21 antibody, clone LB21 recognizes the human Complement receptor type 2, also known as CD21 or the Epstein-Barr virus receptor. CD21 is a ~14 kDa cell surface glycoprotein expressed by mature B cells and by follicular dendritic cells. The molecule acts as a receptor for complement components C3d, C3dg and iC3b, as well as for Epstein Barr Virus. It forms part of a large signal transduction complex in association with CD19. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Mink Bovine Goat Cat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Human IM9 cell line.
- Approx. Protein Concentrations
- IgG concentration 25 ug/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | |
| Immunofluorescence | |
||
| Immunohistology - Frozen 1 | |
1/10 | 1/100 |
| Immunohistology - Paraffin | |||
| Immunoprecipitation | |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl human whole blood
- Histology Positive Control Tissue
- Lymph node
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD21 antibody
-
Furukawa, Y. et al. (2000) Frequent reversible membrane damage in peripheral blood B cells in human T cell lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP).
Clin Exp Immunol. 120 (2): 307-16. -
Aichem, A. et al. (2006) Redox regulation of CD21 shedding involves signaling via PKC and indicates the formation of a juxtamembrane stalk.
J Cell Sci. 119: 2892-902. -
Sengstake, S. et al. (2006) CD21 and CD62L shedding are both inducible via P2X7Rs.
Int Immunol. 18: 1171-8. -
Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
Vet Immunol Immunopathol. 119: 115-22. -
Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37. -
Sopp, P. et al. (2007) Cross-reactivity of mAbs to human CD antigens with cells from cattle.
Vet Immunol Immunopathol. 119: 106-14. -
Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
Vet Immunol Immunopathol. 119: 123-30. -
Angel, C.E. et al. (2009) Distinctive localization of antigen-presenting cells in human lymph nodes.
Blood. 113: 1257-67.
View The Latest Product References
-
Meister, R.K. et al. (2007) Progress in the discovery and definition of monoclonal antibodies for use in feline research.
Vet Immunol Immunopathol. 119: 38-46. -
Clutterbuck, E.A. et al. (2012) Pneumococcal conjugate and plain polysaccharide vaccines have divergent effects on antigen-specific B cells.
J Infect Dis. 205: 1408-16. -
Damgaard, B.M. et al. (2012) The effects of feed restriction on physical activity, body weight, physiology, haematology and immunology in female mink.
Res Vet Sci. 93: 936-42. -
Stachowiak, B. & Weingartl, H.M. (2012) Nipah virus infects specific subsets of porcine peripheral blood mononuclear cells.
PLoS One. 7 (1): e30855. -
Pooley, H.B. et al. (2022) Sheep vaccinated against paratuberculosis have increased levels of B cells infiltrating the intestinal tissue.
Vet Immunol Immunopathol. 252: 110482.
- Synonyms
- CR2
- RRID
- AB_321346
- UniProt
- P20023
- Entrez Gene
- CR2
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004875 complement receptor activity
- GO:0006958 complement activation, classical pathway
- GO:0042803 protein homodimerization activity
- GO:0045087 innate immune response
MCA1195
149544
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