CD21 antibody | LB21

Product Code Applications Pack Size List Price Your Price Qty
MCA1195
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SDS Safety Datasheet SDS
C * F IF IP 2 ml loader
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Mouse anti Human CD21 antibody, clone LB21 recognizes the human Complement receptor type 2, also known as CD21 or the Epstein-Barr virus receptor. CD21 is a ~14 kDa cell surface glycoprotein expressed by mature B cells and by follicular dendritic cells. The molecule acts as a receptor for complement components C3d, C3dg and iC3b, as well as for Epstein Barr Virus. It forms part of a large signal transduction complex in association with CD19.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Mink
Bovine
Goat
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Human IM9 cell line.
Approx. Protein Concentrations
IgG concentration 25 ug/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunofluorescence
Immunohistology - Frozen 1 1/10 1/100
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul human whole blood.
Histology Positive Control Tissue
Lymph node

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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

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Description Mouse IgG1 Negative Control

References for CD21 antibody

  1. Furukawa, Y. et al. (2000) Frequent reversible membrane damage in peripheral blood B cells in human T cell lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP).
    Clin Exp Immunol. 120 (2): 307-16.
  2. Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
    Vet Immunol Immunopathol. 119: 115-22.
  3. Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
    Vet Immunol Immunopathol. 119: 27-37.
  4. Sopp, P. et al. (2007) Cross-reactivity of mAbs to human CD antigens with cells from cattle.
    Vet Immunol Immunopathol. 119: 106-14.
  5. Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
    Vet Immunol Immunopathol. 119: 123-30.
  6. Aichem, A. et al. (2006) Redox regulation of CD21 shedding involves signaling via PKC and indicates the formation of a juxtamembrane stalk.
    J Cell Sci. 119: 2892-902.
  7. Angel, C.E. et al. (2009) Distinctive localization of antigen-presenting cells in human lymph nodes.
    Blood. 113: 1257-67.
  8. Sengstake, S. et al. (2006) CD21 and CD62L shedding are both inducible via P2X7Rs.
    Int Immunol. 18: 1171-8.
  9. View The Latest Product References
  10. Clutterbuck, E.A. et al. (2012) Pneumococcal conjugate and plain polysaccharide vaccines have divergent effects on antigen-specific B cells.
    J Infect Dis. 205: 1408-16.
  11. Meister, R.K. et al. (2007) Progress in the discovery and definition of monoclonal antibodies for use in feline research.
    Vet Immunol Immunopathol. 119: 38-46.
  12. Damgaard, B.M. et al. (2012) The effects of feed restriction on physical activity, body weight, physiology, haematology and immunology in female mink.
    Res Vet Sci. 93: 936-42.
  13. Stachowiak, B. & Weingartl, H.M. (2012) Nipah virus infects specific subsets of porcine peripheral blood mononuclear cells.
    PLoS One. 7 (1): e30855.

Immunofluorescence

Synonyms
CR2
RRID
AB_321346
UniProt
P20023
Entrez Gene
CR2
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004875 complement receptor activity
GO:0006958 complement activation, classical pathway
GO:0042803 protein homodimerization activity
GO:0045087 innate immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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