CD21 antibody | Bu33
Mouse anti Human CD21
- Product Type
- Monoclonal Antibody
- Clone
- Bu33
- Isotype
- IgG1
- Specificity
- CD21
Antibody Quality and Performance Guarantee
| Mouse anti Human CD21, clone Bu33 recognizes human CD21, a ~145 kDa cell surface glycoprotein expressed by mature B lymphocytes, follicular dendritic cells, subsets of normal thymocytes and subsets of T cells. The CD21 molecule acts as a receptor for CD23, for complement components C3d, C3dg and iC3b and for Epstein Barr Virus. CD21 is part of the signalling complex that is linked to CD19, CD81 and phosphatidylinositol-3-kinase. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Horse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
| Immunohistology - Paraffin | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD21 antibody
-
Ling, N.R. et al. (1998) Origin and properties of soluble CD21 (CR2) in human blood.
Clin Exp Immunol. 113 (3): 360-6. -
Garland Publishing, Inc. (1998) Leucocyte Typing VI Garland Publishing, Inc.
pp140-142 -
Wei, F. et al. (2001) A man who inherited his SRY gene and Leri-Weill dyschondrosteosis from his mother and neurofibromatosis type 1 from his father.
Am J Med Genet. 102 (4): 353-8. -
Mayall, S. et al. (2001) The anti-human CD21 antibody, Bu33, identifies equine B cells.
J Comp Pathol. 124 (1): 83-7. -
Thornton, C.A. et al. (2002) Expression of CD21 and CD23 during human fetal development.
Pediatr Res. 52 (2): 245-50.
- Synonyms
- CR2
- RRID
- AB_905940
- UniProt
- P20023
- Entrez Gene
- CR2
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004875 complement receptor activity
- GO:0006958 complement activation, classical pathway
- GO:0042803 protein homodimerization activity
- GO:0045087 innate immune response
MCA2499
148574
155643
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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