Mouse anti Human CD21, clone Bu33 recognizes human CD21, a ~145 kDa cell surface glycoprotein expressed by mature B lymphocytes, follicular dendritic cells, subsets of normal thymocytes and subsets of T cells. The CD21 molecule acts as a receptor for CD23, for complement components C3d, C3dg and iC3b and for Epstein Barr Virus. CD21 is part of the signalling complex that is linked to CD19, CD81 and phosphatidylinositol-3-kinase.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
complement receptor activity
complement activation, classical pathway
protein homodimerization activity
innate immune response
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD21 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD21 antibody
Oxford University Press. (1997) Leucocyte Typing III.
Garland Publishing, Inc. (1998) Leucocyte Typing VI.
Thornton, C.A. et al. (2002) Expression of CD21 and CD23 during human fetal development.
Pediatr Res. 52 (2): 245-50.
Mayall, S. et al. (2001) The anti-human CD21 antibody, Bu33, identifies equine B cells.
J Comp Pathol. 124 (1): 83-7.
Ling, N.R. et al. (1998) Origin and properties of soluble CD21 (CR2) in human blood.
Clin Exp Immunol. 113 (3): 360-6.
Wei, F. et al. (2001) A man who inherited his SRY gene and Leri-Weill dyschondrosteosis from his mother and neurofibromatosis type 1 from his father.
Am J Med Genet. 102 (4): 353-8.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra