CD206 antibody | 7-450

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Mouse anti Human CD206

Product Type
Monoclonal Antibody
Clone
7-450
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2519 C 0.2 mg
MCA2519GA C 0.1 mg
Mouse anti Human CD206 antibody, clone 7-450 recognizes human CD206, otherwise known as macrophage mannose receptor (MMR), a transmembrane protein and member of the multilectin family of pattern recognition receptors (PRRs), which mediates phagocytosis and endocytosis, and is modulated by a range of factors including cytokines, immunoglobulin receptors and pathogens.

CD206 has two extracellular lectin binding sites: the cysteine-rich domain binds to sulphated sugars of glycoproteins such as thyroid stimulating hormone, whilst the recognition of mannose and fucose occurs through carbohydrate recognition domains (CRDs). The widespread distribution and recognition of a wide range of antigens by macrophages, plays a key role in both innate and adaptive immunity, with CD206 able to bind to both gram-negative and gram-positive bacteria, mycobacterium, yeasts, fungi and parasites. The expression of CD206 is not restricted to macrophages, but is also present on immature dendritic cells (DCs), endothelial cells, kidney mesangial cells, retinal pigment epithelium and tracheal smooth muscle cells.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Monocyte-derived dendritic cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P22897 Related reagents
Entrez Gene
MRC1 Related reagents
GO Terms
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005537 mannose binding
GO:0006898 receptor-mediated endocytosis
Regulatory
For research purposes only

Applications of CD206 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/50 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Product Specific References

Further Reading

  1. Stahl, P.D. & Ezekowitz, R.A. (1998) The mannose receptor is a pattern recognition receptor involved in host defense.
    Curr Opin Immunol. 10 (1): 50-5.
  2. Gordon, S. (1995) The macrophage.
    Bioessays. 17 (11): 977-86.