CD200R antibody | OX108

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Anti Human CD200R Antibody, clone OX108 thumbnail image 1

Anti Human CD200R Antibody, clone OX108 thumbnail image 2

Anti Human CD200R Antibody, clone OX108 thumbnail image 3

Anti Human CD200R Antibody, clone OX108 gallery image 1 — Figure A. FITC conjugated Mouse anti Human CD4 (MCA1267F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Human CD4 (MCA1267F) and Alexa Fluor 647 conjugated Mouse anti Human CD200R (MCA2282A647). All experiments performed on human blood gated on live, single lymphocytes in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer

Anti Human CD200R Antibody, clone OX108 gallery image 2 — **Published customer image:**
Mouse anti Human CD200R antibody, clone OX-108 (**MCA2282**) used for the identification of M2 macrophages in synovial tissue by immunofluorescence.
**Image caption:**
Double immunofluorescence stainings of CD68 and macrophage polarization markers on synovial tissue from chronic inflammatory arthritis. Colocalization of CD68 (green) with MΦIFN-γ marker CD64 (A), MΦIL-4 markers CD200R (B) and CD14 (C), and MΦIL-10 markers CD163 (D), CD16 (E), and CD32 (F) (red) on synovial tissue macrophages. Figures are representative of five spondyloarthritis (SpA) and five rheumatoid arthritis (RA) patients. Higher-magnification photos are included in each figure part.

From: Ambarus CA, Noordenbos T, de Hair MJ, Tak PP, Baeten DL.
Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
Arthritis Res Ther. 2012 Apr 11;14(2):R74.

Anti Human CD200R Antibody, clone OX108 gallery image 3 — **Published customer image:**
Mouse anti Human CD200R antibody, clone OX108 used for the identification of M2 macrophages in synovial tissue by immunofluorescence.
**Image caption:**
Double-immunofluorescence stainings of MΦIFN-γ, MΦIL-4, and MΦIL-10 polarization markers on synovial tissue from chronic inflammatory arthritis. Colocalization of MΦIFN-γ marker CD64 with CD200R (A), CD163 (B), and CD32 (C), colocalization of MΦIL-4 marker CD200R with CD14 (D), CD163 (E), and CD32 (F), and colocalization of MΦIL-10 marker CD163 with CD14 (G) and CD32 (H). Figure parts are representative of five spondyloarthritis (SpA) and five rheumatoid arthritis (RA) patients.

From: Ambarus CA, Noordenbos T, de Hair MJ, Tak PP, Baeten DL. Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
Arthritis Res Ther. 2012 Apr 11;14(2):R74.

Mouse anti Human CD200R:Alexa Fluor® 488

Mouse anti Human CD200R:Alexa Fluor® 647

Mouse anti Human CD200R:Biotin

Mouse anti Human CD200R

Mouse anti Human CD200R:RPE

Product Type: Monoclonal Antibody
Clone: OX108
Isotype: IgG1

Product Code	Applications	Pack Size
MCA2282A488T	F	25 Tests/0.25ml
MCA2282A488	F	100 Tests/1ml
MCA2282A647T	F	25 Tests/0.25ml
MCA2282A647	F	100 Tests/1ml
MCA2282B	F	0.1 mg
MCA2282T	F	25 µg
MCA2282	F	0.2 mg
MCA2282PET	F	25 Tests
MCA2282PE	F	100 Tests

Mouse anti Human CD200R antibody, clone OX108 recognizes human CD200R, a cell surface glycoprotein (also known as OX2R). In humans CD200R is expressed primarily by peripheral blood monocytes and neutrophils but also by other leucocytes including T-lymphocytes and mast cells, CD200-CD200R interaction may be involved in the control of myeloid cellular function (Wright et al. 2003).

Levels of expression on resting peripheral blood cells are relatively low.

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Alexa Fluor® 488 - liquid

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Product Form

Purified IgG conjugated to Biotin - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Pack Size: 25 Tests

Reconstitute in 0.25 ml disilled water

Pack Size: 100 Tests

Reconstitute with 1.0 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Carrier Free

Yes

Immunogen

Fusion protein hCD200RCD4d3+4.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC.

DO NOT FREEZE

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: Q8TD46 Related reagents
Entrez Gene: CD200R1 Related reagents
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD200R antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry		Neat
Flow Cytometry	Neat	1/5 Pack Size: 25 Tests/0.25ml 1/10 Pack Size: 100 Tests/1ml
Flow Cytometry		Neat
Flow Cytometry	1/50	1/100
Flow Cytometry	Neat	1/10

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:Alexa Fluor® 488	MCA928A488	100 Tests/1ml	F
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	100 Tests/1ml	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Product Specific References

References for CD200R antibody

Wright, G.J. et al. (2003) Characterization of the CD200 receptor family in mice and humans and their interactions with CD200.
J Immunol. 171 (6): 3034-46.
Koning, N. et al. (2009) Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions.
J Neuropathol Exp Neurol. 68: 159-67.
Melief, J. et al. (2013) Microglia in normal appearing white matter of multiple sclerosis are alerted but immunosuppressed.
Glia. 61 (11): 1848-61.
Ambarus, C.A. et al. (2012) Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
Arthritis Res Ther. 14 (2): R74.
Holmannova, D. et al. (2015) Inhibitory CD200R and proapoptotic CD95/CD95L molecules on innate immunity cells are modulated by cardiac surgery.
Perfusion. 30 (7): 543-55.
Ambarus CA et al. (2012) Soluble immune complexes shift the TLR-induced cytokine production of distinct polarized human macrophage subsets towards IL-10.
PLoS One. 7 (4): e35994.
Memarian, A. et al. (2013) Upregulation of CD200 is associated with Foxp3+ regulatory T cell expansion and disease progression in acute myeloid leukemia.
Tumour Biol. 34 (1): 531-42.
Sousa, S. et al. (2015) Human breast cancer cells educate macrophages toward the M2 activation status.
Breast Cancer Res. 17 (1): 101.
Vicetti Miguel, R.D. et al. (2013) Human female genital tract infection by the obligate intracellular bacterium Chlamydia trachomatis elicits robust Type 2 immunity.
PLoS One. 8 (3): e58565.
van de Garde, M.D. et al. (2014) Chronic exposure to glucocorticoids shapes gene expression and modulates innate and adaptive activation pathways in macrophages with distinct changes in leukocyte attraction.
J Immunol. 192 (3): 1196-208.
Melief, J. et al. (2012) Phenotyping primary human microglia: tight regulation of LPS responsiveness.
Glia. 60 (10): 1506-17.
Georgoudaki, A.M. et al. (2016) Reprogramming Tumor-Associated Macrophages by Antibody Targeting Inhibits Cancer Progression and Metastasis.
Cell Rep. 15 (9): 2000-11.
BjörnfotHolmström, S. et al. (2017) Gingival Tissue Inflammation Promotes Increased Matrix Metalloproteinase-12 Production by CD200R^low Monocyte-Derived Cells in Periodontitis.
J Immunol. Nov 03 [Epub ahead of print].

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