CD200R antibody | OX108
Mouse anti Human CD200R antibody, clone OX-108 (MCA2282) used for the identification of M2 macrophages in synovial tissue by immunofluorescence.
Image caption:
Double immunofluorescence stainings of CD68 and macrophage polarization markers on synovial tissue from chronic inflammatory arthritis. Colocalization of CD68 (green) with MΦIFN-γ marker CD64 (A), MΦIL-4 markers CD200R (B) and CD14 (C), and MΦIL-10 markers CD163 (D), CD16 (E), and CD32 (F) (red) on synovial tissue macrophages. Figures are representative of five spondyloarthritis (SpA) and five rheumatoid arthritis (RA) patients. Higher-magnification photos are included in each figure part.
From: Ambarus CA, Noordenbos T, de Hair MJ, Tak PP, Baeten DL.
Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
Arthritis Res Ther. 2012 Apr 11;14(2):R74.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD200R antibody, clone OX108 used for the identification of M2 macrophages in synovial tissue by immunofluorescence.
Image caption:
Double-immunofluorescence stainings of MΦIFN-γ, MΦIL-4, and MΦIL-10 polarization markers on synovial tissue from chronic inflammatory arthritis. Colocalization of MΦIFN-γ marker CD64 with CD200R (A), CD163 (B), and CD32 (C), colocalization of MΦIL-4 marker CD200R with CD14 (D), CD163 (E), and CD32 (F), and colocalization of MΦIL-10 marker CD163 with CD14 (G) and CD32 (H). Figure parts are representative of five spondyloarthritis (SpA) and five rheumatoid arthritis (RA) patients.
From: Ambarus CA, Noordenbos T, de Hair MJ, Tak PP, Baeten DL. Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
Arthritis Res Ther. 2012 Apr 11;14(2):R74.
AlexaFluor 647 conjugated Mouse anti Human CD200R antibody, clone OX-108 (MCA2282A647) used for the evaluation of CD200R expression on endometrial tissue by flow cytometry.
Image caption:
Endometrial Chlamydia infection promotes alternative activation of macrophages.
Endometrial tissue from women with no identified C. trachomatis, N. gonorrhoeae, or T. vaginalis lower or upper genital tract infection (n = 4), or from women with endocervical or endometrial C. trachomatis infection (n = 14 for Panel A; n = 12 for Panel B) were processed for flow cytometric analysis as described in Methods section. Macrophages were identified as FSC-AintSSC-AintCD45+CD15-CD14+HLA-DR+ live cells (as depicted in Figure S3), and 2 monoclonal antibody panels were used to interrogate macrophage differentiation and activation. Panel (A) evaluated expression of CD163, CD209, CD200R and CD206. Comparisons were done using unpaired one-tailed Student t-tests with Welch’s correction (horizontal bars indicate mean values for each group and significant p values are indicated in bold characters). Open circles indicate samples from uninfected controls; light gray circles indicate samples from women with cervical Chlamydia infection; and dark gray circles indicate samples from women with endometrial Chlamydia infection. Representative contour plots of CD200R, CD206, CD40 and CD80 expression by endometrial macrophages are displayed next to figures. For CD200R and CD206 expression evaluation (A), representative flow plots from peripheral blood monocytes treated with IL-4 (100 U/ml) for 24 hours and the corresponding untreated control are also shown.
From:Vicetti Miguel RD, Harvey SAK, LaFramboise WA, Reighard SD, Matthews DB, Cherpes TL (2013)
Human Female Genital Tract Infection by the Obligate Intracellular Bacterium Chlamydia trachomatis Elicits Robust Type 2 Immunity.
PLoS ONE 8(3): e58565.
doi: 10.1371/journal.pone.0058565
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD200R antibody, clone OX108 (MCA2282) used to label CD200R expressing cells in buman brain biopsies by immunohistochemistry on cryostat sections.
Image caption:
Gene and protein expression of CD200, CD47, CD200R and SIRPα in NAGM
SIRPα was expressed by Iba1+ microglia; scale bar is set at 10 μm. DAB stainings showed (M) mainly extracellular deposition and sporadic expression on neurons for CD200, (N) mainly extracellular deposition and sporadic expression on neurons for CD47, (O) sporadic positive staining of cells for CD200R, and (P) light positive neurons and some darker stained round cells for SIRPα. Scale bars are set at 20 μm. .
From: van den Bosch AMR, Wever D, Schonewille P, Schuller SL, Smolders J, Hamann J, Huitinga I.
Cortical CD200-CD200R and CD47-SIRPα expression is associated with multiple sclerosis pathology.
Brain Commun. 2024 Aug 7;6(4):fcae264.
doi: 10.1093/braincomms/fcae264.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD200R antibody, clone OX108 (MCA2282) used to label CD200R expressing cells in buman brain biopsies by immunofluorescence.
Image caption:
Gene and protein expression of CD200, CD47, CD200R and SIRPα in NAGM
(K) CD200R was expressed by Iba1+ microglia; scale bar is set at 10 μm. (L) SIRPα was expressed by Iba1+ microglia; scale bar is set at 10 μm.
From: van den Bosch AMR, Wever D, Schonewille P, Schuller SL, Smolders J, Hamann J, Huitinga I.
Cortical CD200-CD200R and CD47-SIRPα expression is associated with multiple sclerosis pathology.
Brain Commun. 2024 Aug 7;6(4):fcae264.
doi: 10.1093/braincomms/fcae264.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD200R
- Product Type
- Monoclonal Antibody
- Clone
- OX108
- Isotype
- IgG1
- Specificity
- CD200R
Antibody Quality and Performance Guarantee
| Mouse anti Human CD200R antibody, clone OX108 recognizes human CD200R, a cell surface glycoprotein (also known as OX2R). In humans CD200R is expressed primarily by peripheral blood monocytes and neutrophils but also by other leucocytes including T-lymphocytes and mast cells, CD200-CD200R interaction may be involved in the control of myeloid cellular function (Wright et al. 2003). Levels of expression on resting peripheral blood cells are relatively low. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Fusion protein hCD200RCD4d3+4.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD200R antibody
-
Wright, G.J. et al. (2003) Characterization of the CD200 receptor family in mice and humans and their interactions with CD200.
J Immunol. 171 (6): 3034-46. -
Koning, N. et al. (2009) Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions.
J Neuropathol Exp Neurol. 68: 159-67. -
Melief, J. et al. (2012) Phenotyping primary human microglia: tight regulation of LPS responsiveness.
Glia. 60 (10): 1506-17. -
Ambarus CA et al. (2012) Soluble immune complexes shift the TLR-induced cytokine production of distinct polarized human macrophage subsets towards IL-10.
PLoS One. 7 (4): e35994. -
Ambarus, C.A. et al. (2012) Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
Arthritis Res Ther. 14 (2): R74. -
Vicetti, R.M. et al. (2013) Human female genital tract infection by the obligate intracellular bacterium
Chlamydia trachomatis
elicits robust Type 2 immunity.
PLoS One. 8 (3): e58565. -
Melief, J. et al. (2013) Microglia in normal appearing white matter of multiple sclerosis are alerted but immunosuppressed.
Glia. 61 (11): 1848-61. -
Vicetti Miguel, R.D. et al. (2013) Human female genital tract infection by the obligate intracellular bacterium Chlamydia trachomatis elicits robust Type 2 immunity.
PLoS One. 8 (3): e58565.
View The Latest Product References
-
Memarian, A. et al. (2013) Upregulation of CD200 is associated with Foxp3+ regulatory T cell expansion and disease progression in acute myeloid leukemia.
Tumour Biol. 34 (1): 531-42. -
van de Garde, M.D. et al. (2014) Chronic exposure to glucocorticoids shapes gene expression and modulates innate and adaptive activation pathways in macrophages with distinct changes in leukocyte attraction.
J Immunol. 192 (3): 1196-208. -
Sousa, S. et al. (2015) Human breast cancer cells educate macrophages toward the M2 activation status.
Breast Cancer Res. 17 (1): 101. -
Holmannova, D. et al. (2015) Inhibitory CD200R and proapoptotic CD95/CD95L molecules on innate immunity cells are modulated by cardiac surgery.
Perfusion. 30 (7): 543-55. -
Wiedemann, G.M. et al. (2016) A novel TLR7 agonist reverses NK cell anergy and cures RMA-S lymphoma-bearing mice.
Oncoimmunology. 5 (7): e1189051. -
Björnfot Holmström, S. et al. (2017) Gingival Tissue Inflammation Promotes Increased Matrix Metalloproteinase-12 Production by CD200Rlow Monocyte-Derived Cells in Periodontitis.
J Immunol. 199 (12): 4023-35. -
Pascoal, M.R. et al. (2022) CD200R1L is a functional evolutionary conserved activating receptor in human neutrophils.
J Leukoc Biol. 111 (2): 367-77. -
Sun, H. et al. (2020) The CD200/CD200R signaling pathway contributes to spontaneous functional recovery by enhancing synaptic plasticity after stroke.
J Neuroinflammation. 17 (1): 171. -
van den Bosch, A.M.R. et al. (2024) Cortical CD200-CD200R and CD47-SIRPα expression is associated with multiple sclerosis pathology.
Brain Commun. 6 (4): fcae264. -
Fraser, S.D. et al. (2021) Distinct immune regulatory receptor profiles linked to altered monocyte subsets in sarcoidosis.
ERJ Open Res.7 (1): 00804-2020.
MCA2282
153109
MCA2282T
156350
1610
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