CD200 antibody | OX-104

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Anti Human CD200 Antibody, clone OX-104 thumbnail image 1

Anti Human CD200 Antibody, clone OX-104 thumbnail image 2

Anti Human CD200 Antibody, clone OX-104 gallery image 1 — Figure A. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and A488 conjugated mouse IgG1 isotype control (MCA928A488). Figure B. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and A488 conjugated Mouse anti Human CD200 (**MCA1960A488**). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5™ cell analyzer

Anti Human CD200 Antibody, clone OX-104 gallery image 2 — Figure A. FITC conjugated Mouse anti Human CD19 (MCA1940F) and PE-Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928P647). Figure B. FITC conjugated Mouse anti Human CD19 (MCA1940F) and PE-Alexa Fluor 647 conjugated Mouse anti Human CD200 (MCA1960P647). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer

Mouse anti Human CD200:Alexa Fluor® 488

Mouse anti Human CD200:Alexa Fluor® 647

Mouse anti Human CD200:FITC

Mouse anti Human CD200

Mouse anti Human CD200:RPE

Mouse anti Human CD200:RPE-Alexa Fluor® 647

Product Type: Monoclonal Antibody
Clone: OX-104
Isotype: IgG1

Product Code	Applications	Pack Size
MCA1960A488	F	100 Tests/1ml
MCA1960A647T	F	25 Tests/0.25ml
MCA1960A647	F	100 Tests/1ml
MCA1960FT	F	25 µg
MCA1960F	F	0.1 mg
MCA1960T	C F	25 µg
MCA1960GA	C F	0.1 mg
MCA1960	C F	0.2 mg
MCA1960PE	F	100 Tests
MCA1960P647	F	100 Tests

Mouse anti Human CD200 antibody, clone OX-104 recognizes the human CD200 cell surface antigen, also known as OX2.

CD200 is expressed by a subset of B lymphocytes, some endothelial cells and by neurons. Studies have suggested that the CD200-CD200 ligand system is of importance in the control of macrophage and granulocyte activation.

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Alexa Fluor 488 - liquid

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Product Form

Purified IgG conjugated to RPE-Alexa Fluor 647 - lyophilized

Reconstitution

Reconstitute with 1ml distilled water

Reconstitution

Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Carrier Free

Yes

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee: 18 months from date of despatch
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution.
Guarantee: 12 months from date of reconstitution

More Information

UniProt: P41217 Related reagents
Entrez Gene: CD200 Related reagents
GO Terms: GO:0005887 integral to plasma membrane; GO:0050776 regulation of immune response
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD200 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat
Flow Cytometry		Neat
Flow Cytometry	Neat	1/5
Flow Cytometry	1/50	1/100
Immunohistology - Frozen
Flow Cytometry		Neat
Flow Cytometry	Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:Alexa Fluor® 488	MCA928A488	100 Tests/1ml	F
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	100 Tests/1ml	F
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F
Mouse IgG1 Negative Control:RPE-Alexa Fluor® 647	MCA928P647	100 Tests/1ml	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD200 antibody

Wright, G.J. et al. (2001) The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.
Immunology 102 (2): 173-9.
Ko, Y.C. et al. (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
J Anat. 214: 183-95.
Koning, N. et al. (2009) Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions.
J Neuropathol Exp Neurol. 68: 159-67.
Koning, N. et al. (2007) Downregulation of macrophage inhibitory molecules in multiple sclerosis lesions.
Ann Neurol. 62: 504-14.
Raftery, M.J. et al. (2004) Shaping phenotype, function, and survival of dendritic cells by cytomegalovirus-encoded IL-10.
J Immunol. 173: 3383-91.
Meuth, S.G. et al. (2008) CNS inflammation and neuronal degeneration is aggravated by impaired CD200-CD200R-mediated macrophage silencing.
J Neuroimmunol. 194: 62-9.
Yamauchi, K. and Kurosaka, A. (2010) Expression and function of glycogen synthase kinase-3 in human hair follicles.
Arch Dermatol Res. 302: 263-70.
Kloepper, J.E. et al. (2008) Immunophenotyping of the human bulge region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche.
Exp Dermatol. 17: 592-609.
Ohyama, M. et al. (2006) Characterization and isolation of stem cell-enriched human hair follicle bulge cells.
J Clin Invest. 116: 249-60.
Darmochwal-Kolarz, D. et al. (2013) The expressions of co-stimulatory molecules are altered on putative antigen-presenting cells in cord blood.
Am J Reprod Immunol. 69 (2): 180-7.
Colmont, C.S. et al. (2013) CD200-expressing human basal cell carcinoma cells initiate tumor growth.
Proc Natl Acad Sci U S A. 110 (4): 1434-9.
Chen, H.J. et al. (2015) Human placenta-derived adherent cells improve cardiac performance in mice with chronic heart failure.
Stem Cells Transl Med. 4 (3): 269-75.
Ohyama, M. & Kobayashi, T. (2012) Isolation and characterization of stem cell-enriched human and canine hair follicle keratinocytes.
Methods Mol Biol. 879: 389-401.
Patel, G.K. et al. (2012) Identification and characterization of tumor-initiating cells in human primary cutaneous squamous cell carcinoma.
J Invest Dermatol. 132 (2): 401-9.
Kloepper, J.E. et al. (2008) Immunophenotyping of the human bulge region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche.
Exp Dermatol. 17 (7): 592-609.

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