CD200 antibody | OX-104
Mouse anti Human CD200 antibody, clone OX-104 (MCA1960) used to label CD200 expressing cells in buman brain biopsies by immunofluorescence.
Image caption:
Gene and protein expression of CD200, CD47, CD200R and SIRPα in NAGM.
(E) Immunofluorescent staining of CD200 and MBP shows that CD200 expression was abundant in NAGM and low in NAWM. Scale bar on the overview image is 1 cm and on the zoomed image it is 250 μm. (F) CD200 expression co-localized with MBP+ myelin as indicated with arrows; scale bar is set at 10 μm. (G) CD200 expression co-localized with NFH+ axons as indicated with arrows; scale bar is set at 5 μm. (H) CD47 expression was, like CD200, abundant in NAGM and lower in NAWM. Scale bar on the overview image is 1 cm and on the zoomed image is 250 μm. (I) CD47 strongly co-localized with some MBP+ myelin, as indicated with the arrows, and was sometimes localized adjacent to the myelin, on the outer layer, as indicated with the arrowhead. Scale bar is set at 10 μm. (J) Occasionally, CD47 co-localized with NFH+ axons, scale bar is set at 5 μm.
From: van den Bosch AMR, Wever D, Schonewille P, Schuller SL, Smolders J, Hamann J, Huitinga I.
Cortical CD200-CD200R and CD47-SIRPα expression is associated with multiple sclerosis pathology.
Brain Commun. 2024 Aug 7;6(4):fcae264.
doi: 10.1093/braincomms/fcae264.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD200 antibody, clone OX-104 (MCA1960) used to label CD200 expressing cells in buman brain biopsies by immunohistochemistry on cryostat sections.
Image caption:
SIRPα was expressed by Iba1+ microglia; scale bar is set at 10 μm. DAB stainings showed (M) mainly extracellular deposition and sporadic expression on neurons for CD200, (N) mainly extracellular deposition and sporadic expression on neurons for CD47, (O) sporadic positive staining of cells for CD200R, and (P) light positive neurons and some darker stained round cells for SIRPα. Scale bars are set at 20 μm.
From: van den Bosch AMR, Wever D, Schonewille P, Schuller SL, Smolders J, Hamann J, Huitinga I.
Cortical CD200-CD200R and CD47-SIRPα expression is associated with multiple sclerosis pathology.
Brain Commun. 2024 Aug 7;6(4):fcae264.
doi: 10.1093/braincomms/fcae264.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD200 antibody, clone OX-104 (MCA1960) used to label CD200 expressing cells in buman brain biopsies by immunohistochemistry on cryostat sections.
Image caption:
Serial dilutions of CD200 for optical density analysis, and optical density at [1:80,000] measured over DAB incubation time.
From: van den Bosch AMR, Wever D, Schonewille P, Schuller SL, Smolders J, Hamann J, Huitinga I.
Cortical CD200-CD200R and CD47-SIRPα expression is associated with multiple sclerosis pathology.
Brain Commun. 2024 Aug 7;6(4):fcae264.
doi: 10.1093/braincomms/fcae264.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD200
- Product Type
- Monoclonal Antibody
- Clone
- OX-104
- Isotype
- IgG1
- Specificity
- CD200
Antibody Quality and Performance Guarantee
| Mouse anti Human CD200 antibody, clone OX-104 recognizes the human CD200 cell surface antigen, also known as OX2. CD200 is expressed by a subset of B lymphocytes, some endothelial cells and by neurons. The CD200-CD200 ligand system is of importance in the control of macrophage and granulocyte activation. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD200 antibody
-
Wright, G.J. et al. (2001) The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.
Immunology 102 (2): 173-9. -
Raftery, M.J. et al. (2004) Shaping phenotype, function, and survival of dendritic cells by cytomegalovirus-encoded IL-10.
J Immunol. 173: 3383-91. -
Ohyama, M. et al. (2006) Characterization and isolation of stem cell-enriched human hair follicle bulge cells.
J Clin Invest. 116: 249-60. -
Koning, N. et al. (2007) Downregulation of macrophage inhibitory molecules in multiple sclerosis lesions.
Ann Neurol. 62: 504-14. -
Kloepper, J.E. et al. (2008) Immunophenotyping of the human bulge region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche.
Exp Dermatol. 17 (7): 592-609. -
Kloepper, J.E. et al. (2008) Immunophenotyping of the human bulge region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche.
Exp Dermatol. 17: 592-609. -
Meuth, S.G. et al. (2008) CNS inflammation and neuronal degeneration is aggravated by impaired CD200-CD200R-mediated macrophage silencing.
J Neuroimmunol. 194: 62-9. -
Koning, N. et al. (2009) Distribution of the immune inhibitory molecules CD200 and CD200R in the normal central nervous system and multiple sclerosis lesions suggests neuron-glia and glia-glia interactions.
J Neuropathol Exp Neurol. 68: 159-67.
View The Latest Product References
-
Ko, Y.C. et al. (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
J Anat. 214: 183-95. -
Yamauchi, K. and Kurosaka, A. (2010) Expression and function of glycogen synthase kinase-3 in human hair follicles.
Arch Dermatol Res. 302: 263-70. -
Patel, G.K. et al. (2012) Identification and characterization of tumor-initiating cells in human primary cutaneous squamous cell carcinoma.
J Invest Dermatol. 132 (2): 401-9. -
Ohyama, M. & Kobayashi, T. (2012) Isolation and characterization of stem cell-enriched human and canine hair follicle keratinocytes.
Methods Mol Biol. 879: 389-401. -
Colmont, C.S. et al. (2013) CD200-expressing human basal cell carcinoma cells initiate tumor growth.
Proc Natl Acad Sci U S A. 110 (4): 1434-9. -
Darmochwal-Kolarz, D. et al. (2013) The expressions of co-stimulatory molecules are altered on putative antigen-presenting cells in cord blood.
Am J Reprod Immunol. 69 (2): 180-7. -
Chen, H.J. et al. (2015) Human placenta-derived adherent cells improve cardiac performance in mice with chronic heart failure.
Stem Cells Transl Med. 4 (3): 269-75. -
Bertolini, M. et al. (2023) Mechanical epilation exerts complex biological effects on human hair follicles and perifollicular skin: An ex vivo study approach.
Int J Cosmet Sci. Nov 03 [Epub ahead of print]. -
van den Bosch, A.M.R. et al. (2024) Cortical CD200-CD200R and CD47-SIRPα expression is associated with multiple sclerosis pathology.
Brain Commun. 6 (4): fcae264.
- Synonyms
- OX2
- RRID
- AB_321839
- UniProt
- P41217
- Entrez Gene
- CD200
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0050776 regulation of immune response
View more products with CD200 specificity
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