CD1a antibody | NA1/34-HLK
Image caption:
Endometrial CD4+cells express CCR5. (a) Scattered CD4+ cells were detected in the luminal and glandular epithelium as well as both stromal locations (lamina superficialis and lamina basalis). (b) Clusters of CD4+ cells were also detected just beneath the luminal epithelium and (c) adjacent to glandular epithelium. (d) A majority of the scattered CD4+ cells (red) co-expressed (yellow) CD3+ (green). (e) The lymphoid aggregate formations contained vast numbers of CD3+ cells (red) and occasional CD1a+ cells (green). (f) A positive co-localization (yellow) of CCR5+ cells (red) and CD4+ cells (green) was frequently observed both in the luminal epithelium and underlying stroma and (g) in the lymphoid aggregate formations. Arrowheads indicate example of double-positive staining; arrows indicate single-positive staining. Scale bars: a: 500 μm; b, c: 100 μm; d–f: 25 μm.
From: Kaldensjö T, Petersson P, Tolf A, Morgan G, Broliden K, et al. (2011)
Detection of Intraepithelial and Stromal Langerin and CCR5 Positive Cells in the Human Endometrium: Potential Targets for HIV Infection.
PLoS ONE 6(6): e21344.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Image caption:
Distribution of antigen-presenting cell subsets in human endometrium. (a) CD1a+ cells were rare and mainly found within the luminal or glandular epithelium. (b) CD1a+ cells (green) co-expressed (yellow) CD11c+ (red), although not all CD11c+ cells expressed CD1a. (c) The CD1a+ cells (green) also expressed CD4 (red). (d) CD11c+ and (e) CD68+ cells were present in much higher frequencies in both the epithelium and stroma and clusters of both (f) CD11c+ and (g) CD68+ cells were present in the lamina basalis adjacent to glandular epithelium (marked area: 7.1×104 μm2 and 8.1×104 μm2, respectively). (h) CD123+ cells were solely present in the stroma as scattered cells and in streak formations. (i) Some but not all CD123+ (red) cells co-localized (yellow) with BDCA-2 (green). BDCA-2+ (green) neither co-localized with (j) CD11c+ (red) nor (k) CD68+ (red) cells, although they were occasionally detected in close proximity to each other. Arrowheads indicate example of positive staining. Scale bars: a: 500 μm; b, c, j, k: 25 μm; d–i: 100 μm.
From: Kaldensjö T, Petersson P, Tolf A, Morgan G, Broliden K, et al. (2011)
Detection of Intraepithelial and Stromal Langerin and CCR5 Positive Cells in the Human Endometrium: Potential Targets for HIV Infection.
PLoS ONE 6(6): e21344.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Image caption:
CLR expression on various DC subsets present in the endometrium. (a) Langerin positive cells were mainly detected within the luminal and glandular epithelium. Positive co-localization (yellow) was detected with Langerin+ cells (green) and (b) CD1a (red) and (c) CD11c (red). (d) Double-positive cells (yellow) expressing Langerin+ (green) and CD4+ (red) cells were also detected within CD4+ lymphoid aggregates. (e) DC-SIGN and (f) MR were detected in the lamina superficialis, just beneath the luminal epithelium (I: minimum distance of cell body to endometrial lumen: 49 µm and 40 µm, respectively). Both (g) DC-SIGN (green) and (h) MR(green) were co-expressed (yellow) on CD68+ cells (red) as well as (i and j, respectively) CD11c+ cells (red). Neither (k) DC-SIGN (red) nor (l) MR (red) were expressed on CD1a+ (green) cells. Scale bars: a: 500 μm; b–d: 25 µm; e–l: 100 µm.
From: Kaldensjö T, Petersson P, Tolf A, Morgan G, Broliden K, et al. (2011)
Detection of Intraepithelial and Stromal Langerin and CCR5 Positive Cells in the Human Endometrium: Potential Targets for HIV Infection.
PLoS ONE 6(6): e21344.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
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F C IF P ResetMouse anti Human CD1a
- Product Type
- Monoclonal Antibody
- Clone
- NA1/34-HLK
- Isotype
- IgG2a
- Specificity
- CD1a
Antibody Quality and Performance Guarantee
| Mouse anti Human CD1a antibody, clone NA1/34-HLK recognizes the human CD1a cell surface glycoprotein, a ~49 kDa single pass type 1 transmembrane glycoprotein containing a single Ig-like domain, expressed in association with beta 2 microglobulin. CD1a is expressed strongly by cortical thymocytes, and also by Langerhans cells and interdigitating cells. CD1a is involved in the presentation of lipids and glycolipids to NK cells ( |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Human thymocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NS1/1 Ag4.1 mouse myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Skin
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
Source Reference
-
McMichael, A.J. et al. (1979) A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody.
Eur J Immunol. 9 (3): 205-10.
References for CD1a antibody
-
Poulter, L.W. et al. (1986) Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies.
Scand J Immunol. 24 (3): 351-7. -
Scheinecker, C. et al. (1998) Initiation of the autologous mixed lymphocyte reaction requires the expression of costimulatory molecules B7-1 and B7-2 on human peripheral blood dendritic cells.
J Immunol. 161: 3966-73. -
Murray, S. et al. (2000) Diagnostic and therapeutic evaluation of an anti-Langerhans cell histiocytosis monoclonal antibody (NA1/34) in a new xenograft model.
J Invest Dermatol. 114: 127-34. -
Buettner, M. et al. (2005) Inverse correlation of maturity and antibacterial activity in human dendritic cells.
J Immunol. 174: 4203-9. -
Cox, K. et al. (2005) Plasmacytoid dendritic cells (PDC) are the major DC subset innately producing cytokines in human lymph nodes.
J Leukoc Biol. 78: 1142-52. -
Angel, C.E. et al. (2006) Distinctive localization of antigen-presenting cells in human lymph nodes.
Blood. 113: 1257-67. -
Wang, Y.S. et al. (2007) Characterization of canine monocyte-derived dendritic cells with phenotypic and functional differentiation.
Can J Vet Res. 71: 165-74. -
Angel, C.E. et al. (2007) Comprehensive analysis of MHC-II expression in healthy human skin.
Immunol Cell Biol. 85: 363-9.
View The Latest Product References
-
Angel, C.E. et al. (2007) CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts.
Int Immunol. 19: 1271-9. -
Elia, A.R. et al. (2008) Human dendritic cells differentiated in hypoxia down-modulate antigen uptake and change their chemokine expression profile.
J Leukoc Biol. 84: 1472-82. -
Liu, C.C. et al (2008) Transient downregulation of monocyte-derived dendritic-cell differentiation, function, and survival during tumoral progression and regression in an in vivo canine model of transmissible venereal tumor.
Cancer Immunol Immunother. 57: 479-91. -
Fanales-Belasio, E. et al. (2009) HIV-1 Tat addresses dendritic cells to induce a predominant Th1-type adaptive immune response that appears prevalent in the asymptomatic stage of infection.
J Immunol. 182: 2888-97. -
Sugiura K et al. (2010) Effect of IL-12 on canine dendritic cell maturation following differentiation induced by granulocyte-macrophage CSF and IL-4.
Vet Immunol Immunopathol. 137 (3-4): 322-6. -
Mito, K. et al. (2010) IFN{gamma} markedly cooperates with intratumoral dendritic cell vaccine in dog tumor models.
Cancer Res. 70: 7093-101. -
Hirbod, T. et al. (2010) Abundant expression of HIV target cells and C-type lectin receptors in the foreskin tissue of young Kenyan men.
Am J Pathol. 176: 2798-805. -
Kaldensjö, T. et al. (2011) Detection of intraepithelial and stromal Langerin and CCR5 positive cells in the human endometrium: potential targets for HIV infection.
PLoS One. 6: e21344. -
Bosco, M.C. et al. (2011) Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker in vitro and in vivo.
Blood. 117: 2625-39. -
Baharom, F. et al. (2016) Dendritic Cells and Monocytes with Distinct Inflammatory Responses Reside in Lung Mucosa of Healthy Humans.
J Immunol. 196 (11): 4498-509. -
Bonnefont-Rebeix, C. et al. (2016) Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.
Immunobiology. 221 (1): 12-22. -
Zegarska, B. et al. (2017) Changes of Langerhans cells during skin ageing.
Postepy Dermatol Alergol. 34 (3): 260-7. -
Tomić, S. et al. (2018) Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.
Int J Nanomedicine. 13: 6941-60.
- RRID
- AB_321221
- UniProt
- P06126
- Entrez Gene
- CD1A
- GO Terms
- GO:0042612 MHC class I protein complex
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0010008 endosome membrane
- GO:0019882 antigen processing and presentation
MCA80GA
148547
156517
MCA80
156346
1806
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