CD1a antibody | NA1/34-HLK
Image caption:
Endometrial CD4+cells express CCR5. (a) Scattered CD4+ cells were detected in the luminal and glandular epithelium as well as both stromal locations (lamina superficialis and lamina basalis). (b) Clusters of CD4+ cells were also detected just beneath the luminal epithelium and (c) adjacent to glandular epithelium. (d) A majority of the scattered CD4+ cells (red) co-expressed (yellow) CD3+ (green). (e) The lymphoid aggregate formations contained vast numbers of CD3+ cells (red) and occasional CD1a+ cells (green). (f) A positive co-localization (yellow) of CCR5+ cells (red) and CD4+ cells (green) was frequently observed both in the luminal epithelium and underlying stroma and (g) in the lymphoid aggregate formations. Arrowheads indicate example of double-positive staining; arrows indicate single-positive staining. Scale bars: a: 500 µm; b, c: 100 µm; d–f: 25 µm.
From: Kaldensjö T, Petersson P, Tolf A, Morgan G, Broliden K, et al. (2011) Detection of Intraepithelial and Stromal Langerin and CCR5 Positive Cells in the Human Endometrium: Potential Targets for HIV Infection. PLoS ONE 6(6): e21344.
Image caption:
Distribution of antigen-presenting cell subsets in human endometrium. (a) CD1a+ cells were rare and mainly found within the luminal or glandular epithelium. (b) CD1a+ cells (green) co-expressed (yellow) CD11c+ (red), although not all CD11c+ cells expressed CD1a. (c) The CD1a+ cells (green) also expressed CD4 (red). (d) CD11c+ and (e) CD68+ cells were present in much higher frequencies in both the epithelium and stroma and clusters of both (f) CD11c+ and (g) CD68+ cells were present in the lamina basalis adjacent to glandular epithelium (marked area: 7.1×104 µm2 and 8.1×104 µm2, respectively). (h) CD123+ cells were solely present in the stroma as scattered cells and in streak formations. (i) Some but not all CD123+ (red) cells co-localized (yellow) with BDCA-2 (green). BDCA-2+ (green) neither co-localized with (j) CD11c+ (red) nor (k) CD68+ (red) cells, although they were occasionally detected in close proximity to each other. Arrowheads indicate example of positive staining. Scale bars: a: 500 µm; b, c, j, k: 25 µm; d–i: 100 µm.
From: Kaldensjö T, Petersson P, Tolf A, Morgan G, Broliden K, et al. (2011) Detection of Intraepithelial and Stromal Langerin and CCR5 Positive Cells in the Human Endometrium: Potential Targets for HIV Infection. PLoS ONE 6(6): e21344.
Image caption:
CLR expression on various DC subsets present in the endometrium. (a) Langerin positive cells were mainly detected within the luminal and glandular epithelium. Positive co-localization (yellow) was detected with Langerin+ cells (green) and (b) CD1a (red) and (c) CD11c (red). (d) Double-positive cells (yellow) expressing Langerin+ (green) and CD4+ (red) cells were also detected within CD4+ lymphoid aggregates. (e) DC-SIGN and (f) MR were detected in the lamina superficialis, just beneath the luminal epithelium (I: minimum distance of cell body to endometrial lumen: 49 µm and 40 µm, respectively). Both (g) DC-SIGN (green) and (h) MR(green) were co-expressed (yellow) on CD68+ cells (red) as well as (i and j, respectively) CD11c+ cells (red). Neither (k) DC-SIGN (red) nor (l) MR (red) were expressed on CD1a+ (green) cells. Scale bars: a: 500 µm; b–d: 25 µm; e–l: 100 µm.
From: Kaldensjö T, Petersson P, Tolf A, Morgan G, Broliden K, et al. (2011) Detection of Intraepithelial and Stromal Langerin and CCR5 Positive Cells in the Human Endometrium: Potential Targets for HIV Infection. PLoS ONE 6(6): e21344.
Mouse anti Human CD1a:Alexa Fluor® 488
Mouse anti Human CD1a:Alexa Fluor® 647
Mouse anti Human CD1a:FITC
Mouse anti Human CD1a:Pacific Blue®
Mouse anti Human CD1a
Mouse anti Human CD1a:RPE
Mouse anti Human CD1a:RPE-Alexa Fluor® 750
- Product Type
- Monoclonal Antibody
- Clone
- NA1/34-HLK
- Isotype
- IgG2a
Mouse anti Human CD1a antibody, clone NA1/34-HLK recognizes the human CD1a cell surface glycoprotein, a ~49 kDa single pass type 1 transmembrane glycoprotein containing a single Ig-like domain, expressed in association with beta 2 microglobulin. CD1a is expressed strongly by cortical thymocytes, and also by Langerhans cells and interdigitating cells. CD1a is involved in the presentation of lipids and glycolipids to NK cells (Sloma et al. 2008).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
- Product Form
- Purified IgG conjugated to Pacific Blue - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to RPE-Alexa Fluor 750 - lyophilized
- Reconstitution
- Pack Size: 25 TestsReconstitute in 0.25 ml disilled waterPack Size: 100 TestsReconstitute with 1 ml distilled water.
- Reconstitution
- Reconstitute with 1.0 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by ion exchange chromatography.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1.0% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Human thymocytes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NS1/1 Ag4.1 mouse myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
- Shelf Life
- 12 months from date of reconstitution
More Information
- UniProt
- P06126 Related reagents
- Entrez Gene
- CD1A Related reagents
- GO Terms
- GO:0042612 MHC class I protein complex
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0010008 endosome membrane
- GO:0019882 antigen processing and presentation
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD1a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Histology Positive Control Tissue
- Skin
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Alexa Fluor® 488 | MCA929A488 | 100 Tests/1ml | F | ||
| Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA929A647 | 100 Tests/1ml | F | ||
| Mouse IgG2a Negative Control:FITC | MCA929F | 100 Tests | F | ||
| Mouse IgG2a Negative Control:Pacific Blue® | MCA929PB | 100 Tests/1ml | F | ||
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F | ||
| Mouse IgG2a Negative Control:RPE | MCA929PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for CD1a antibody
-
McMichael, A.J. et al. (1979) A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody.
Eur J Immunol. 9 (3): 205-10. -
Poulter, L.W. et al. (1986) Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies.
Scand J Immunol. 24 (3): 351-7. -
Galkowska, H. et al. (1996) Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes.
Vet Immunol Immunopathol. 53 (3-4): 329-34. -
Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Exp Anim. 49 (2): 97-110. -
Hirbod, T. et al. (2010) Abundant expression of HIV target cells and C-type lectin receptors in the foreskin tissue of young Kenyan men.
Am J Pathol. 176: 2798-805. -
Liu, C.C. et al (2008) Transient downregulation of monocyte-derived dendritic-cell differentiation, function, and survival during tumoral progression and regression in an in vivo canine model of transmissible venereal tumor.
Cancer Immunol Immunother. 57: 479-91. -
Sugiura K et al. (2010) Effect of IL-12 on canine dendritic cell maturation following differentiation induced by granulocyte-macrophage CSF and IL-4.
Vet Immunol Immunopathol. 137 (3-4): 322-6. -
Angel, C.E. et al. (2006) Distinctive localization of antigen-presenting cells in human lymph nodes.
Blood. 113: 1257-67. -
Bosco, M.C. et al. (2011) Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker in vitro and in vivo.
Blood. 117: 2625-39. -
Buettner, M. et al. (2005) Inverse correlation of maturity and antibacterial activity in human dendritic cells.
J Immunol. 174: 4203-9. -
Cox, K. et al. (2005) Plasmacytoid dendritic cells (PDC) are the major DC subset innately producing cytokines in human lymph nodes.
J Leukoc Biol. 78: 1142-52. -
Mito, K. et al. (2010) IFN{gamma} markedly cooperates with intratumoral dendritic cell vaccine in dog tumor models.
Cancer Res. 70: 7093-101. -
Murray, S. et al. (2000) Diagnostic and therapeutic evaluation of an anti-Langerhans cell histiocytosis monoclonal antibody (NA1/34) in a new xenograft model.
J Invest Dermatol. 114: 127-34. -
Scheinecker, C. et al. (1998) Initiation of the autologous mixed lymphocyte reaction requires the expression of costimulatory molecules B7-1 and B7-2 on human peripheral blood dendritic cells.
J Immunol. 161: 3966-73. -
Wang, Y.S. et al. (2007) Characterization of canine monocyte-derived dendritic cells with phenotypic and functional differentiation.
Can J Vet Res. 71: 165-74. -
Elia, A.R. et al. (2008) Human dendritic cells differentiated in hypoxia down-modulate antigen uptake and change their chemokine expression profile.
J Leukoc Biol. 84: 1472-82. -
Fanales-Belasio, E. et al. (2009) HIV-1 Tat addresses dendritic cells to induce a predominant Th1-type adaptive immune response that appears prevalent in the asymptomatic stage of infection.
J Immunol. 182: 2888-97. -
Kaldensjö, T. et al. (2011) Detection of intraepithelial and stromal Langerin and CCR5 positive cells in the human endometrium: potential targets for HIV infection.
PLoS One. 6: e21344. -
Angel, C.E. et al. (2007) CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts.
Int Immunol. 19: 1271-9. -
Angel, C.E. et al. (2007) Comprehensive analysis of MHC-II expression in healthy human skin.
Immunol Cell Biol. 85: 363-9. -
Baharom F. et al. (2016) Dendritic Cells and Monocytes with Distinct Inflammatory Responses Reside in Lung Mucosa of Healthy Humans
The Journal of Immunology. May 2 [Epub ahead of print] -
Bonnefont-Rebeix, C. et al. (2016) Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.
Immunobiology. 221 (1): 12-22. -
Zegarska, B. et al. (2017) Changes of Langerhans cells during skin ageing.
Postepy Dermatol Alergol. 34 (3): 260-7.
