CD1a antibody | NA1/34-HLK

Mouse anti Human CD1a

Product Type
Monoclonal Antibody
Clone
NA1/34-HLK
Isotype
IgG2a
Specificity
CD1a

Product Code Applications Pack Size List Price Your Price Qty
MCA80GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF 0.1 mg loader
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loader
MCA80
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD1a antibody, clone NA1/34-HLK recognizes the human CD1a cell surface glycoprotein, a ~49 kDa single pass type 1 transmembrane glycoprotein containing a single Ig-like domain, expressed in association with beta 2 microglobulin. CD1a is expressed strongly by cortical thymocytes, and also by Langerhans cells and interdigitating cells. CD1a is involved in the presentation of lipids and glycolipids to NK cells (Sloma et al. 2008).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human thymocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the NS1/1 Ag4.1 mouse myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Skin

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References for CD1a antibody

  1. McMichael, A.J. et al. (1979) A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody.
    Eur J Immunol. 9 (3): 205-10.
  2. Poulter, L.W. et al. (1986) Discrimination of human macrophages and dendritic cells by means of monoclonal antibodies.
    Scand J Immunol. 24 (3): 351-7.
  3. Galkowska, H. et al. (1996) Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes.
    Vet Immunol Immunopathol. 53 (3-4): 329-34.
  4. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  5. Hirbod, T. et al. (2010) Abundant expression of HIV target cells and C-type lectin receptors in the foreskin tissue of young Kenyan men.
    Am J Pathol. 176: 2798-805.
  6. Liu, C.C. et al (2008) Transient downregulation of monocyte-derived dendritic-cell differentiation, function, and survival during tumoral progression and regression in an in vivo canine model of transmissible venereal tumor.
    Cancer Immunol Immunother. 57: 479-91.
  7. Sugiura K et al. (2010) Effect of IL-12 on canine dendritic cell maturation following differentiation induced by granulocyte-macrophage CSF and IL-4.
    Vet Immunol Immunopathol. 137 (3-4): 322-6.
  8. Angel, C.E. et al. (2006) Distinctive localization of antigen-presenting cells in human lymph nodes.
    Blood. 113: 1257-67.
  9. View The Latest Product References
  10. Bosco, M.C. et al. (2011) Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker in vitro and in vivo.
    Blood. 117: 2625-39.
  11. Buettner, M. et al. (2005) Inverse correlation of maturity and antibacterial activity in human dendritic cells.
    J Immunol. 174: 4203-9.
  12. Cox, K. et al. (2005) Plasmacytoid dendritic cells (PDC) are the major DC subset innately producing cytokines in human lymph nodes.
    J Leukoc Biol. 78: 1142-52.
  13. Mito, K. et al. (2010) IFN{gamma} markedly cooperates with intratumoral dendritic cell vaccine in dog tumor models.
    Cancer Res. 70: 7093-101.
  14. Murray, S. et al. (2000) Diagnostic and therapeutic evaluation of an anti-Langerhans cell histiocytosis monoclonal antibody (NA1/34) in a new xenograft model.
    J Invest Dermatol. 114: 127-34.
  15. Scheinecker, C. et al. (1998) Initiation of the autologous mixed lymphocyte reaction requires the expression of costimulatory molecules B7-1 and B7-2 on human peripheral blood dendritic cells.
    J Immunol. 161: 3966-73.
  16. Wang, Y.S. et al. (2007) Characterization of canine monocyte-derived dendritic cells with phenotypic and functional differentiation.
    Can J Vet Res. 71: 165-74.
  17. Elia, A.R. et al. (2008) Human dendritic cells differentiated in hypoxia down-modulate antigen uptake and change their chemokine expression profile.
    J Leukoc Biol. 84: 1472-82.
  18. Fanales-Belasio, E. et al. (2009) HIV-1 Tat addresses dendritic cells to induce a predominant Th1-type adaptive immune response that appears prevalent in the asymptomatic stage of infection.
    J Immunol. 182: 2888-97.
  19. Kaldensjö, T. et al. (2011) Detection of intraepithelial and stromal Langerin and CCR5 positive cells in the human endometrium: potential targets for HIV infection.
    PLoS One. 6: e21344.
  20. Angel, C.E. et al. (2007) CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts.
    Int Immunol. 19: 1271-9.
  21. Angel, C.E. et al. (2007) Comprehensive analysis of MHC-II expression in healthy human skin.
    Immunol Cell Biol. 85: 363-9.
  22. Baharom F. et al. (2016) Dendritic Cells and Monocytes with Distinct Inflammatory Responses Reside in Lung Mucosa of Healthy Humans
    The Journal of Immunology. May 2 [Epub ahead of print]
  23. Bonnefont-Rebeix, C. et al. (2016) Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.
    Immunobiology. 221 (1): 12-22.
  24. Zegarska, B. et al. (2017) Changes of Langerhans cells during skin ageing.
    Postepy Dermatol Alergol. 34 (3): 260-7.
  25. Tomić, S. et al. (2018) Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.
    Int J Nanomedicine. 13: 6941-60.

Flow Cytometry

Immunofluorescence

RRID
AB_321221
UniProt
P06126
Entrez Gene
CD1A
GO Terms
GO:0042612 MHC class I protein complex
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0010008 endosome membrane
GO:0019882 antigen processing and presentation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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