CD186 antibody | rD02-7B3

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Mouse anti Human CD186

Product Type: Monoclonal Antibody
Clone: rD02-7B3
Isotype: IgG1
Specificity: CD186

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TZA093GA Datasheet SDS SDS	F	0.1 mg		Log in
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Anti Human CD186 Antibody, clone rD02-7B3 thumbnail image 1

Anti Human CD186 Antibody, clone rD02-7B3 thumbnail image 2

Anti Human CD186 Antibody, clone rD02-7B3 thumbnail image 3

Anti Human CD186 Antibody, clone rD02-7B3 thumbnail image 4

Anti Human CD186 Antibody, clone rD02-7B3 thumbnail image 5

Anti Human CD186 Antibody, clone rD02-7B3 thumbnail image 6

Anti Human CD186 Antibody, clone rD02-7B3 thumbnail image 7

Anti Human CD186 Antibody, clone rD02-7B3 gallery image 1 — Human PBMCs were stained with Anti-Human CD3 SBUV445 (**MCA463SBUV445**) only (Figure A) or with Anti-Human CD3 SBUV445 (**MCA463SBUV445**) and Anti-Human CD186 SBUV795 (**TZA093SBUV795**) (Figure B). Experiments were performed on PBMCs stimulated for nine days with IL-2, gated on live, single cell lymphocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD186 Antibody, clone rD02-7B3 gallery image 2 — Human PBMCs were stained with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) only (Figure A) or with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) and Anti-Human CD186 SBV515 (**TZA093SBV515**) (Figure B). Experiments were performed on PBMCs stimulated for nine days with IL-2, gated on live, single cell lymphocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD186 Antibody, clone rD02-7B3 gallery image 3 — Human PBMCs were stained with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) only (Figure A) or with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) and Anti-Human CD186 SBV610 (**TZA093SBV610**) (Figure B). Experiments were performed on PBMCs stimulated for nine days with IL-2, gated on live, single cell lymphocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD186 Antibody, clone rD02-7B3 gallery image 4 — Human PBMCs were stained with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) only (Figure A) or with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) and Anti-Human CD186 SBB580 (**TZA093SBB580**) (Figure B). Experiments were performed on PBMCs stimulated for nine days with IL-2, gated on live, single cell lymphocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD186 Antibody, clone rD02-7B3 gallery image 5 — Human PBMCs were stained with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) only (Figure A) or with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) and Anti-Human CD186 SBB615 (**TZA093SBB615**) (Figure B). Experiments were performed on PBMCs stimulated for nine days with IL-2, gated on live, single cell lymphocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD186 Antibody, clone rD02-7B3 gallery image 6 — Human PBMCs were stained with Anti-Human CD3 SBUV400 (**MCA6146SBUV400**) only (Figure A) or with Anti-Human CD3 SBUV400 (**MCA6146SBUV400**) and Anti-Human CD186 SBY720 (**TZA093SBY720**) (Figure B). Experiments were performed on PBMCs stimulated for nine days with IL-2, gated on live, single cell lymphocytes. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human CD186 Antibody, clone rD02-7B3 gallery image 7 — Human PBMCs were stained with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) only (Figure A) or with Anti-Human CD3 SBUV795 (**MCA6146SBUV795**) and Anti-Human CD186 SBUV445 (**TZA093SBUV445**) (Figure B). Experiments were performed on PBMCs stimulated for nine days with IL-2, gated on live, single cell lymphocytes. Data were acquired on the **ZE5 Cell Analyzer**.

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Mouse anti Human CD186 antibody, clone rD02-7B3, is a recombinant monoclonal antibody that recognizes CD186, also known as C-X-C chemokine receptor type 6 (CXCR6), Bonzo or STRL33.

CD186 is a 39kDa G protein-coupled chemokine receptor which is expressed on CD4+ T cells, NK cells and plasma cells, as well as lymphoid tissues. It is expressed at a very low level on naïve CD8+ T cells, with expression upregulated after activation. Through interactions with its ligand CXCL16, CD186 is involved in chemotaxis, aiding in immune responses by recruiting cells to sites of inflammation.

CD186 acts as a co-receptor for m-tropic HIV-1 and SIVs in conjunction with CD4. Dysfunction or altered expression of CD186 has been linked to several autoimmune and inflammatory diseases. In rheumatoid arthritis, elevated expression is found in T cells found in synovial fluid, where it is implicated in disease pathogenesis by inducing T-lymphocyte homing to the joints and Th1 polarization. Expression is also upregulated on T cells in the colons of patients with Crohn’s disease. CD8+ T cell trafficking to the skin in vitiligo patients is mediated by CD186-CXCL16 interactions.

Target Species: Human
Product Form: Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Source: CHO cells
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Mammalian cells transfected with full length CD186
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 0.5x10⁶ cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

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Synonyms: CXCR6
UniProt: O00574
Entrez Gene: CXCR6
GO Terms: GO:0005887 integral to plasma membrane; GO:0019079 viral genome replication; GO:0015026 coreceptor activity; GO:0016493 C-C chemokine receptor activity