CD185 antibody | rB1-10/4F11-35
Mouse anti Human CD185
- Product Type
- Monoclonal Antibody
- Clone
- rB1-10/4F11-35
- Isotype
- IgG1
- Specificity
- CD185
Antibody Quality and Performance Guarantee
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| Mouse anti Human CD185 antibody, clone rB1-10/4F11-35, is a recombinant monoclonal antibody that recognizes CD185, also known as CXCR5. CD185 is a seven transmembrane GPCR belonging to the CXC chemokine receptor family. It is highly expressed in mature circulating B cells, Burkitt lymphoma cells, subpopulations of CD4+ T cells and skin-derived migratory dendritic cells. CD185 binds to B-lymphocyte chemoattractant (CXCR13), leading to multiple cellular events including cell migration, survival, proliferation and modulation of gene expression. CD185 is involved in B-cell migration into B-cell follicles of the spleen and Peyer patches. It is up-regulated on effector and memory T cells after TCR stimulation, whereas IL-2 causes down-regulation. CD185 expression by TfH cells guides their migration toward B cell follicles. It has been reported that CD185 can be used as a marker to discriminate between different B cell subsets. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Mammalian cells transfected with full length human CD185
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
- Synonyms
- CXCR5
- UniProt
- P32302
- Entrez Gene
- CXCR5
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0006928 cellular component movement
- GO:0016494 C-X-C chemokine receptor activity
- GO:0042113 B cell activation
TZA089GA
64702101
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