CD177 antibody | MEM-166

Product Code Applications Pack Size List Price Your Price Qty
MCA2045GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP WB * 0.1 mg loader
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loader
MCA2045
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP WB * 0.2 mg loader
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loader

Mouse anti Human CD177 antibody, clone MEM-166 recognizes human CD177 (neutrophil glycoprotein NB1). The neutrophil NB1 antigen is expressed by 97% of the caucasian population. Antibodies against NB1 have been implicated in the pathology of neonatal alloimmune neutropenia (Lalezari et al. 1971).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
Human granulocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunoprecipitation
Western Blotting 1 Non-reducing conditions
  1. 1MEM-166 recognizes CD177 under non-reducing conditions
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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References for CD177 antibody

  1. Caruccio, L. et al. (2003) Expression of human neutrophil antigen-2a (NB1) is increased in pregnancy.
    Transfusion. 43 (3): 357-63.
  2. Kissel, K. et al. (2002) Molecular basis of NB1 (HNA-2a, CD177) deficiency.
    Blood. 99 (11): 4231-3.
  3. Jerke, U. et al. (2011) Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation.
    J Biol Chem. 286 (9): 7070-81.
  4. Dillon, M. et al. (2008) Expression of the GPI-anchored receptor Prv-1 enhances thrombopoietin and IL-3-induced proliferation in hematopoietic cell lines.
    Leuk Res. 32: 811-9.
  5. Gabillet, J. et al. (2012) Proteinase 3, the autoantigen in granulomatosis with polyangiitis, associates with calreticulin on apoptotic neutrophils, impairs macrophage phagocytosis, and promotes inflammation.
    J Immunol. 189: 2574-83.
  6. Drewniak, A. et al. (2008) Granulocyte concentrates: prolonged functional capacity during storage in the presence of phenotypic changes.
    Haematologica. 93:1058-67.
  7. Drewniak, A. et al. (2009) Changes in gene expression of granulocytes during in vivo granulocyte colony-stimulating factor/dexamethasone mobilization for transfusion purposes.
    Blood. 113: 5979-98.
  8. Sachs, U.J. et al. (2007) The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31).
    J Biol Chem. 282: 23603-12.
  9. View The Latest Product References
  10. Johansson, Å.C. et al. (2016) Impaired phagocytosis and reactive oxygen species production in phagocytes is associated with systemic vasculitis.
    Arthritis Res Ther. 18 (1): 92.
  11. Nishimura, M. et al. (2007) Detection of anti-CD32 alloantibody in donor plasma implicated in development of transfusion-related acute lung injury.
    Cell Biochem Funct. 25 (2): 179-83.
  12. Pliyev, B.K. & Menshikov, M. (2012) Comparative evaluation of the role of the adhesion molecule CD177 in neutrophil interactions with platelets and endothelium.
    Eur J Haematol. 89 (3): 236-44.
  13. Onodera, R. et al. (2017) Anti-human neutrophil antigen-1a, -1b, and -2 antibodies in neonates and children with immune neutropenias analyzed by extracted granulocyte antigen immunofluorescence assay.
    Transfusion. 57 (11): 2586-94.
  14. Bayat, B. et al. (2021) Transfusion of Target Antigens to Pre-Immunized Recipients: A New Mechanism in Transfusion-Related Acute Lung Injury.
    Blood Adv. bloodadvances.2020003843.

Flow Cytometry

Functional Assays

RRID
AB_323350
UniProt
Q8N6Q3
Entrez Gene
CD177
GO Terms
GO:0005886 plasma membrane
GO:0007596 blood coagulation
GO:0031225 anchored to membrane
GO:0050900 leukocyte migration

MCA2045GA

0314 159483 1702

MCA2045

150104 159484 1709

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