CD177 antibody | MEM-166
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Mouse anti Human CD177
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD177 antibody, clone MEM-166 recognizes human CD177 (neutrophil glycoprotein NB1). The neutrophil NB1 antigen is expressed by 97% of the caucasian population. Antibodies against NB1 have been implicated in the pathology of neonatal alloimmune neutropenia (Lalezari et al. 1971).|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rhesus Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Human granulocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Western Blotting 1||Non-reducing conditions|
- 1MEM-166 recognizes CD177 under non-reducing conditions
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD177 antibody
Kissel, K. et al. (2002) Molecular basis of NB1 (HNA-2a, CD177) deficiency.
Blood. 99 (11): 4231-3.
Caruccio, L. et al. (2003) Expression of human neutrophil antigen-2a (NB1) is increased in pregnancy.
Transfusion. 43 (3): 357-63.
Nishimura, M. et al. (2007) Detection of anti-CD32 alloantibody in donor plasma implicated in development of transfusion-related acute lung injury.
Cell Biochem Funct. 25 (2): 179-83.
Sachs, U.J. et al. (2007) The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31).
J Biol Chem. 282: 23603-12.
Drewniak, A. et al. (2008) Granulocyte concentrates: prolonged functional capacity during storage in the presence of phenotypic changes.
Dillon, M. et al. (2008) Expression of the GPI-anchored receptor Prv-1 enhances thrombopoietin and IL-3-induced proliferation in hematopoietic cell lines.
Leuk Res. 32: 811-9.
Drewniak, A. et al. (2009) Changes in gene expression of granulocytes during in vivo granulocyte colony-stimulating factor/dexamethasone mobilization for transfusion purposes.
Blood. 113: 5979-98.
Jerke, U. et al. (2011) Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation.
J Biol Chem. 286 (9): 7070-81.
View The Latest Product References
Pliyev, B.K. & Menshikov, M. (2012) Comparative evaluation of the role of the adhesion molecule CD177 in neutrophil interactions with platelets and endothelium.
Eur J Haematol. 89 (3): 236-44.
Gabillet, J. et al. (2012) Proteinase 3, the autoantigen in granulomatosis with polyangiitis, associates with calreticulin on apoptotic neutrophils, impairs macrophage phagocytosis, and promotes inflammation.
J Immunol. 189: 2574-83.
Johansson, Å.C. et al. (2016) Impaired phagocytosis and reactive oxygen species production in phagocytes is associated with systemic vasculitis.
Arthritis Res Ther. 18 (1): 92.
Onodera, R. et al. (2017) Anti-human neutrophil antigen-1a, -1b, and -2 antibodies in neonates and children with immune neutropenias analyzed by extracted granulocyte antigen immunofluorescence assay.
Transfusion. 57 (11): 2586-94.
Bayat, B. et al. (2021) Transfusion of Target Antigens to Pre-Immunized Recipients: A New Mechanism in Transfusion-Related Acute Lung Injury.
Blood Adv. bloodadvances.2020003843.
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