CD177 antibody | MEM-166
Mouse anti Human CD177 antibody, clone MEM-166 (MCA2045) used for the evaluation of CD177 expression on polymorphonuclear neutrophils by flow cytometry on peripheral blood leukocytes.
Image caption:
Gating strategies to evalate the expression of surface markers
From:Johansson ÅC, Ohlsson S, Pettersson Å, Bengtsson AA, Selga D, Hansson M, Hellmark T.
Impaired phagocytosis and reactive oxygen species production in phagocytes is associated with systemic vasculitis.
Arthritis Res Ther. 2016 Apr 22;18(1):92.
Mouse anti Human CD177
- Product Type
- Monoclonal Antibody
- Clone
- MEM-166
- Isotype
- IgG1
- Specificity
- CD177
Antibody Quality and Performance Guarantee
| Mouse anti Human CD177 antibody, clone MEM-166 recognizes human CD177 (neutrophil glycoprotein NB1). The neutrophil NB1 antigen is expressed by 97% of the caucasian population. Antibodies against NB1 have been implicated in the pathology of neonatal alloimmune neutropenia (Lalezari et al. 1971). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Human granulocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunoprecipitation | |
||
| Western Blotting 1 | |
Non-reducing conditions |
- 1MEM-166 recognizes CD177 under non-reducing conditions
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD177 antibody
-
Kissel, K. et al. (2002) Molecular basis of NB1 (HNA-2a, CD177) deficiency.
Blood. 99 (11): 4231-3. -
Caruccio, L. et al. (2003) Expression of human neutrophil antigen-2a (NB1) is increased in pregnancy.
Transfusion. 43 (3): 357-63. -
Nishimura, M. et al. (2007) Detection of anti-CD32 alloantibody in donor plasma implicated in development of transfusion-related acute lung injury.
Cell Biochem Funct. 25 (2): 179-83. -
Sachs, U.J. et al. (2007) The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31).
J Biol Chem. 282: 23603-12. -
Drewniak, A. et al. (2008) Granulocyte concentrates: prolonged functional capacity during storage in the presence of phenotypic changes.
Haematologica. 93:1058-67. -
Dillon, M. et al. (2008) Expression of the GPI-anchored receptor Prv-1 enhances thrombopoietin and IL-3-induced proliferation in hematopoietic cell lines.
Leuk Res. 32: 811-9. -
Drewniak, A. et al. (2009) Changes in gene expression of granulocytes during in vivo granulocyte colony-stimulating factor/dexamethasone mobilization for transfusion purposes.
Blood. 113: 5979-98. -
Jerke, U. et al. (2011) Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation.
J Biol Chem. 286 (9): 7070-81.
View The Latest Product References
-
Pliyev, B.K. & Menshikov, M. (2012) Comparative evaluation of the role of the adhesion molecule CD177 in neutrophil interactions with platelets and endothelium.
Eur J Haematol. 89 (3): 236-44. -
Gabillet, J. et al. (2012) Proteinase 3, the autoantigen in granulomatosis with polyangiitis, associates with calreticulin on apoptotic neutrophils, impairs macrophage phagocytosis, and promotes inflammation.
J Immunol. 189: 2574-83. -
Johansson, Å.C. et al. (2016) Impaired phagocytosis and reactive oxygen species production in phagocytes is associated with systemic vasculitis.
Arthritis Res Ther. 18 (1): 92. -
Onodera, R. et al. (2017) Anti-human neutrophil antigen-1a, -1b, and -2 antibodies in neonates and children with immune neutropenias analyzed by extracted granulocyte antigen immunofluorescence assay.
Transfusion. 57 (11): 2586-94. -
Bayat, B. et al. (2021) Transfusion of Target Antigens to Pre-Immunized Recipients: A New Mechanism in Transfusion-Related Acute Lung Injury.
Blood Adv. bloodadvances.2020003843.
- RRID
- AB_323350
- UniProt
- Q8N6Q3
- Entrez Gene
- CD177
- GO Terms
- GO:0005886 plasma membrane
- GO:0007596 blood coagulation
- GO:0031225 anchored to membrane
- GO:0050900 leukocyte migration
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