CD172g antibody | OX119

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Mouse anti Human CD172g:Alexa Fluor® 488

Mouse anti Human CD172g:Alexa Fluor® 647

Mouse anti Human CD172g:FITC

Mouse anti Human CD172g

Mouse anti Human CD172g:RPE

Product Type: Monoclonal Antibody
Clone: OX119
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2584A488	F			100 Tests/1ml
MCA2584A647	F			100 Tests/1ml
MCA2584F	F			0.1 mg
MCA2584	F IP			0.2 mg
MCA2584PE	F			100 Tests

Mouse anti Human CD172g antibody, clone OX119 recognizes human SIRP gamma, a 55 kDa signal regulatory protein which is a member of the immunoglobulin gene superfamily. SIRP gamma, also known as CD172g, is expressed on most T lymphocytes and a subset of B-cells but is absent on myeloid cells. CD172g has a truncated cytoplasmic tail, which is similar to SIRP beta, but unlike SIRP beta CD172g does not require DAP12 for expression at the cell surface. CD172g, like SIRP alpha, binds to CD47 inducing apoptosis. It is also involved in the negative regulation of receptor tyrosine kinase-coupled signalling.

Product Details

Target Species

Human

Product Form

Purified IgG conjugated to Alexa Fluor® 488 - liquid

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1.0ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide (NaN₃)
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide (NaN₃)
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide (NaN₃)
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Carrier Free

Yes

Immunogen

Purified recombinant SIRPgamma.CD4.

Approx. Protein Concentrations

IgG concentration 0.05mg/ml

Approx. Protein Concentrations

IgG concentration 0.05mg/ml

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the NS-1 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: Q9P1W8 Related reagents
Entrez Gene: SIRPG Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0005515 protein binding; GO:0007596 blood coagulation; GO:0007155 cell adhesion; GO:0016021 integral to membrane; GO:0035556 intracellular signal transduction; GO:0007267 cell-cell signaling; GO:0008284 positive regulation of cell proliferation; GO:0008285 negative regulation of cell proliferation; GO:0022409 positive regulation of cell-cell adhesion; GO:0034329 cell junction assembly; GO:0050870 positive regulation of T cell activation; GO:0050900 leukocyte migration
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD172g antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/10
Flow Cytometry		Neat	1/10
Flow Cytometry		Neat	1/10
Flow Cytometry		1/50	1/200
Immunoprecipitation
Flow Cytometry		Neat	1/10

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488	MCA928A488	100 Tests/1ml	F
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	100 Tests/1ml	F
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Product Specific References

References for CD172g antibody

Brooke, G. et al. (2004) Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family.
J Immunol.173:2562-70.

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