CD172a antibody | 15-414

Mouse anti Human CD172a

Product Type
Monoclonal Antibody
Clone
15-414
Isotype
IgG2a
Specificity
CD172a

Product Code Applications Pack Size List Price Your Price Qty
MCA2518GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF 0.1 mg loader
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loader
MCA2518
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD172a antibody, clone 15-414 recognizes human CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells.

CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.1 mg
0.09%Sodium Azide (NaN3)
Pack Size: 0.2 mg
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Monocyte-derived dendritic cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 Pack Size: 0.1 mg
1/25 Pack Size: 0.2 mg
1/200 Pack Size: 0.1 mg
1/100 Pack Size: 0.2 mg
Immunofluorescence
Immunohistology - Frozen 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human tonsil.

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Description Goat anti Mouse IgG/A/M:Alk. Phos.
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

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Description Mouse IgG2a Negative Control

References for CD172a antibody

  1. Fujioka, Y. et al. (1996) A novel membrane glycoprotein, SHPS-1, that binds the SH2-domain-containing protein tyrosine phosphatase SHP-2 in response to mitogens and cell adhesion.
    Mol Cell Biol. 16 (12): 6887-99.
  2. Baba, T. et al. (2012) Novel Process of Intrathymic Tumor-Immune Tolerance through CCR2-Mediated Recruitment of Sirpα(+) Dendritic Cells: A Murine Model.
    PLoS One. 7: e41154.
  3. Szaraz, P.et al. (2016) In Vitro Differentiation of First Trimester Human Umbilical Cord Perivascular Cells into Contracting Cardiomyocyte-Like Cells
    Stem Cells International. 2016: 1-13.
  4. Hussen, J. et al. (2014) The chemokine CCL5 induces selective migration of bovine classical monocytes and drives their differentiation into LPS-hyporesponsive macrophages in vitro.
    Dev Comp Immunol. 47 (2): 169-77.

Further Reading

  1. van Beek, E.M. et al. (2005) Signal regulatory proteins in the immune system.
    J Immunol. 175 (12): 7781-7.

Synonyms
SIRP ALPHA
RRID
AB_1055630
UniProt
P78324
Entrez Gene
SIRPA
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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