CD164 antibody | 67D2
Mouse anti Human CD164 clone 67D2, recognizes CD164, also known as endolyn, a 160 kDa transmembrne homodimer protein. It is composed of an extracellular region and two O-glycosylated mucin domains (I and II). These two domains are linked by a non-mucin cysteine rich subdomain.
CD164 is a member of the sialmucin family and is expressed by CD34+ hematopoietic progenitor cells, activated basophils, erythroid cells as well as various cancerous cells. A key role of CD164 is its involvement in regulating adhesion, migration and proliferation of hematopoietic progenitor cells (Zannettino et al. 1998).
Elevated expression of CD164 has been observed in patients with Seazy syndrome which is a primary cutaneous T-cell lymphoma and as such, has been suggested as a potential marker of this disease (Guenova et al. 2016).
This clone CD67D2 recognizes the class II epitope which is not sensitive to sialidase, N-glycanase and O-sialglycoprotease of CD164. This clones has been used in flow cytometry to examine CD164 upregulation in CD203c+ activated basophils (Hennersdorf et al. 2005).
CD164 is a member of the sialmucin family and is expressed by CD34+ hematopoietic progenitor cells, activated basophils, erythroid cells as well as various cancerous cells. A key role of CD164 is its involvement in regulating adhesion, migration and proliferation of hematopoietic progenitor cells (Zannettino et al. 1998).
Elevated expression of CD164 has been observed in patients with Seazy syndrome which is a primary cutaneous T-cell lymphoma and as such, has been suggested as a potential marker of this disease (Guenova et al. 2016).
This clone CD67D2 recognizes the class II epitope which is not sensitive to sialidase, N-glycanase and O-sialglycoprotease of CD164. This clones has been used in flow cytometry to examine CD164 upregulation in CD203c+ activated basophils (Hennersdorf et al. 2005).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Immunogen
- Breast tumor cell line T-47D
- Purity
- >95% by SDS PAGE
- Purity
- >95% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
Applications of CD164 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunocytochemistry | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the undiluted reagent to label 1x106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
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