CD161 antibody | B199.2

Mouse anti Human CD161

Product Type
Monoclonal Antibody
Clone
B199.2
Isotype
IgG1
Specificity
CD161

Product Code Applications Pack Size List Price Your Price Qty
MCA1855
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.2 mg loader
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loader
MCA1855T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 25 µg loader
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loader
MCA1855GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD161 antibody, clone B199.2 recognizes the human Killer cell lectin-like receptor subfamily B member 1, also known as CD161, C-type lectin domain family 5 member B, HNKR-P1a, NKR-P1A or Natural killer cell surface protein P1A. CD161 is a 225 amino acid ~25 kDa predicted molecular mass, single pass type II transmembrane glycoprotein with a single C-type lectin domain. CD161 is expressed by almost all NK cells and a subset of CD3+ve T cells (Lanier 1994).

CD161, a member of the C-lectin is expressed as a disulphide bond-linked homodimeric cell surface protein, comprising two chains of ~40-44 kDa (Lanier et al. 1994). CD161 acts as a receptor for another c-type lectin, LLT1 with roles in the regulation of NK cell and T cell function (Aldemir et al. 2005).

Mouse anti Human CD161 antibody, clone B199.2 cross-competes with and recognizes a similar epitope to the DX1 monoclonal antibody (Lanier et al. 1994).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
MCA1855, MCA1855GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1855T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Purified human NK cells cultured in IL-2 (Bennett et al. 1996)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P2X63.Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Mouse IgG1 Negative Control

References for CD161 antibody

  1. Bennett, I.M. et al. (1996) Definition of a natural killer NKR-P1A+/CD56-/CD16- functionally immature human NK cell subset that differentiates in vitro in the presence of interleukin 12.
    J Exp Med. 184 (5): 1845-56.
  2. Azzoni, L. et al. (1998) Differential transcriptional regulation of CD161 and a novel gene, 197/15a, by IL-2, IL-15, and IL-12 in NK and T cells.
    J Immunol. 161 (7): 3493-500.
  3. de Lalla, C. et al. (2011) Invariant NKT Cell Reconstitution in Pediatric Leukemia Patients Given HLA-Haploidentical Stem Cell Transplantation Defines Distinct CD4+ and CD4- Subset Dynamics and Correlates with Remission State.
    J Immunol. 186: 4490-9.
  4. Huarte, E. et al. (2008) PILAR is a novel modulator of human T-cell expansion.
    Blood. 112: 1259-68.
  5. Williams, P.J. et al. (2009) Altered decidual leucocyte populations in the placental bed in pre-eclampsia and foetal growth restriction: a comparison with late normal pregnancy.
    Reproduction. 138: 177-84.
  6. Higai, K. et al. (2006) Binding of sialyl Lewis X antigen to lectin-like receptors on NK cells induces cytotoxicity and tyrosine phosphorylation of a 17-kDa protein.
    Biochim Biophys Acta. 1760 (9): 1355-63.
  7. Birchall, M.A. et al. (2008) Immunologic response of the laryngeal mucosa to extraesophageal reflux.
    Ann Otol Rhinol Laryngol. 117: 891-5.
  8. Bossard, C. et al. (2012) Plasmacytoid dendritic cells and Th17 immune response contribution in gastrointestinal acute graft-versus-host disease.
    Leukemia. 26: 1471-4.
  9. View The Latest Product References
  10. Richter, J. et al. (2010) CD161 receptor participates in both impairing NK cell cytotoxicity and the response to glycans and vimentin in patients with rheumatoid arthritis.
    Clin Immunol. 136: 139-47.
  11. Abrahamsson, S.V. et al. (2013) Non-myeloablative autologous haematopoietic stem cell transplantation expands regulatory cells and depletes IL-17 producing mucosal-associated invariant T cells in multiple sclerosis.
    Brain. 136: 2888-903.
  12. Rother, S. et al. (2015) The c.503T>C Polymorphism in the Human KLRB1 Gene Alters Ligand Binding and Inhibitory Potential of CD161 Molecules.
    PLoS One. 10 (8): e0135682.

Flow Cytometry

Immunohistology - Frozen

Synonyms
NKR-P1
RRID
AB_323331
UniProt
Q12918
Entrez Gene
KLRB1
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005529 sugar binding
GO:0007166 cell surface receptor linked signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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