CD161 antibody | B199.2
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|Mouse anti Human CD161 antibody, clone B199.2 recognizes the human Killer cell lectin-like receptor subfamily B member 1, also known as CD161, C-type lectin domain family 5 member B, HNKR-P1a, NKR-P1A or Natural killer cell surface protein P1A. CD161 is a 225 amino acid ~25 kDa predicted molecular mass, single pass type II transmembrane glycoprotein with a single C-type lectin domain. CD161 is expressed by almost all NK cells and a subset of CD3+ve T cells (Lanier 1994).
CD161, a member of the C-lectin is expressed as a disulphide bond-linked homodimeric cell surface protein, comprising two chains of ~40-44 kDa (Lanier et al. 1994). CD161 acts as a receptor for another c-type lectin, LLT1 with roles in the regulation of NK cell and T cell function (Aldemir et al. 2005).
Mouse anti Human CD161 antibody, clone B199.2 cross-competes with and recognizes a similar epitope to the DX1 monoclonal antibody (Lanier et al. 1994).
- Target Species
- Product Form
- Purified IgG - liquid
- MCA1855GA, MCA1855: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA1855T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Purified human NK cells cultured in IL-2 (Bennett et al. 1996)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P2X63.Ag8.653 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD161 antibody
Bennett, I.M. et al. (1996) Definition of a natural killer NKR-P1A+/CD56-/CD16- functionally immature human NK cell subset that differentiates in vitro in the presence of interleukin 12.
J Exp Med. 184 (5): 1845-56.
Azzoni, L. et al. (1998) Differential transcriptional regulation of CD161 and a novel gene, 197/15a, by IL-2, IL-15, and IL-12 in NK and T cells.
J Immunol. 161 (7): 3493-500.
de Lalla, C. et al. (2011) Invariant NKT Cell Reconstitution in Pediatric Leukemia Patients Given HLA-Haploidentical Stem Cell Transplantation Defines Distinct CD4+ and CD4- Subset Dynamics and Correlates with Remission State.
J Immunol. 186: 4490-9.
Huarte, E. et al. (2008) PILAR is a novel modulator of human T-cell expansion.
Blood. 112: 1259-68.
Williams, P.J. et al. (2009) Altered decidual leucocyte populations in the placental bed in pre-eclampsia and foetal growth restriction: a comparison with late normal pregnancy.
Reproduction. 138: 177-84.
Higai, K. et al. (2006) Binding of sialyl Lewis X antigen to lectin-like receptors on NK cells induces cytotoxicity and tyrosine phosphorylation of a 17-kDa protein.
Biochim Biophys Acta. 1760 (9): 1355-63.
Birchall, M.A. et al. (2008) Immunologic response of the laryngeal mucosa to extraesophageal reflux.
Ann Otol Rhinol Laryngol. 117: 891-5.
Bossard, C. et al. (2012) Plasmacytoid dendritic cells and Th17 immune response contribution in gastrointestinal acute graft-versus-host disease.
Leukemia. 26: 1471-4.
View The Latest Product References
Richter, J. et al. (2010) CD161 receptor participates in both impairing NK cell cytotoxicity and the response to glycans and vimentin in patients with rheumatoid arthritis.
Clin Immunol. 136: 139-47.
Abrahamsson, S.V. et al. (2013) Non-myeloablative autologous haematopoietic stem cell transplantation expands regulatory cells and depletes IL-17 producing mucosal-associated invariant T cells in multiple sclerosis.
Brain. 136: 2888-903.
Rother, S. et al. (2015) The c.503T>C Polymorphism in the Human KLRB1 Gene Alters Ligand Binding and Inhibitory Potential of CD161 Molecules.
PLoS One. 10 (8): e0135682.
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