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CD155 antibody | SKII.4

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Anti Human CD155 Antibody, clone SKII.4 thumbnail image 1 Anti Human CD155 Antibody, clone SKII.4 thumbnail image 2 Anti Human CD155 Antibody, clone SKII.4 thumbnail image 3 Anti Human CD155 Antibody, clone SKII.4 thumbnail image 4 Anti Human CD155 Antibody, clone SKII.4 thumbnail image 5
Anti Human CD155 Antibody, clone SKII.4 gallery image 1
Human peripheral blood monocytes stained with Mouse anti Human CD155 (MCA4644)
Anti Human CD155 Antibody, clone SKII.4 gallery image 2
Human peripheral blood monocytes stained with Mouse anti Human CD155 (MCA4644GA)
Anti Human CD155 Antibody, clone SKII.4 gallery image 3
Human peripheral blood monocytes stained with Mouse anti Human CD155 Alexa Fluor® 647 (MCA4644A647)
Anti Human CD155 Antibody, clone SKII.4 gallery image 4
Human peripheral blood monocytes stained with Mouse anti Human CD155:Endotoxin Low (MCA4644EL)
Anti Human CD155 Antibody, clone SKII.4 gallery image 5
Human peripheral blood monocytes stained with Mouse anti Human CD155 : Alexa Fluor® 488 (MCA4644A488)

Mouse anti Human CD155:Alexa Fluor® 488

Mouse anti Human CD155:Alexa Fluor® 647

Mouse anti Human CD155:Low Endotoxin

Mouse anti Human CD155

Product Type
Monoclonal Antibody
Clone
SKII.4
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA4644A488
Datasheet
F
SDS
100 Tests/1ml loader

MCA4644A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA4644EL
Datasheet
C F FN IF
SDS
0.5 mg loader

MCA4644GA
Datasheet
C F IF
SDS
0.1 mg loader

MCA4644
Datasheet
C F IF
SDS
0.2 mg loader

Mouse anti Human CD155 antibody, clone SKII.4 recognizes human CD155, otherwise known as poliovirus receptor (PVR). CD155 is a 397 amino acid type I transmembrane glycoprotein with a predicted molecular mass of ~45 kDa, a member of the nectin family, which acts as a mediator of cell-cell adhesion and cell migration, and is a receptor for poliovirus and cytomegalovirus.

CD155 is a primate-restricted marker expressed by monocytes, macrophages, CD34+ thymocytes, endothelial and epithelial cells, and exists as both cell surface (alpha and delta) and secreted (beta and gamma) isoforms. CD155 promotes natural killer (NK) cell adhesion and NK effector functions, interacting with the NK receptors CD96 (Tactile) and CD226 (DNAM-1). CD155 forms a heterodimer with CD113 (nectin-3) which then binds to integrin alphaVbeta3.

Mouse anti Human CD155 antibody, clone SKII.4 has been shown to inhibit NK cell-mediated lysis (Fuchs et al. 2004).

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Human SK-N-S1 neuroblastoma cells.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P15151
Entrez Gene
PVR
GO Terms
GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
GO:0016021 integral to membrane
GO:0050839 cell adhesion molecule binding
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0009986 cell surface
GO:0030054 cell junction
GO:0034329 cell junction assembly
GO:0034332 adherens junction organization
GO:0042271 susceptibility to natural killer cell mediated cytotoxicity
GO:0044419 interspecies interaction between organisms
GO:0060370 susceptibility to T cell mediated cytotoxicity
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD155 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry
Flow Cytometry 1/25 1/200
Functional Assays
Immunofluorescence
Immunohistology - Frozen
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:Low Endotoxin MCA928EL C F 0.5 mg loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Immunohistology - Frozen

Product Specific References

Source Reference

  1. Fuchs, A. et al. (2004) Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155).
    J Immunol. 172 (7): 3994-8.

References for CD155 antibody

  1. Fuchs, A. et al. (2005) Paradoxic inhibition of human natural interferon-producing cells by the activating receptor NKp44.
    Blood. 106 (6): 2076-82.
  2. Boles, K.S. et al. (2009) A novel molecular interaction for the adhesion of follicular CD4 T cells to follicular DC.
    Eur J Immunol. 39 (3): 695-703.
  3. Cella, M. et al. (2010) Loss of DNAM-1 contributes to CD8+ T-cell exhaustion in chronic HIV-1 infection.
    Eur J Immunol. 40 (4): 949-54.

Fluorescent Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra
Request a different product with the CD155 specificity