CD155 antibody | SKII.4
Mouse anti Human CD155:Alexa Fluor® 488
Mouse anti Human CD155:Alexa Fluor® 647
Mouse anti Human CD155:Low Endotoxin
Mouse anti Human CD155
- Product Type
- Monoclonal Antibody
- Clone
- SKII.4
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA4644A488 | F | 100 Tests/1ml |
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| MCA4644A647 | F | 100 Tests/1ml |
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| MCA4644EL | C F FN IF | 0.5 mg |
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| MCA4644GA | C F IF | 0.1 mg |
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| MCA4644 | C F IF | 0.2 mg |
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Mouse anti Human CD155 antibody, clone SKII.4 recognizes human CD155, otherwise known as poliovirus receptor (PVR). CD155 is a 397 amino acid type I transmembrane glycoprotein with a predicted molecular mass of ~45 kDa, a member of the nectin family, which acts as a mediator of cell-cell adhesion and cell migration, and is a receptor for poliovirus and cytomegalovirus.
CD155 is a primate-restricted marker expressed by monocytes, macrophages, CD34+ thymocytes, endothelial and epithelial cells, and exists as both cell surface (alpha and delta) and secreted (beta and gamma) isoforms. CD155 promotes natural killer (NK) cell adhesion and NK effector functions, interacting with the NK receptors CD96 (Tactile) and CD226 (DNAM-1). CD155 forms a heterodimer with CD113 (nectin-3) which then binds to integrin alphaVbeta3.
Mouse anti Human CD155 antibody, clone SKII.4 has been shown to inhibit NK cell-mediated lysis (Fuchs et al. 2004).
CD155 is a primate-restricted marker expressed by monocytes, macrophages, CD34+ thymocytes, endothelial and epithelial cells, and exists as both cell surface (alpha and delta) and secreted (beta and gamma) isoforms. CD155 promotes natural killer (NK) cell adhesion and NK effector functions, interacting with the NK receptors CD96 (Tactile) and CD226 (DNAM-1). CD155 forms a heterodimer with CD113 (nectin-3) which then binds to integrin alphaVbeta3.
Mouse anti Human CD155 antibody, clone SKII.4 has been shown to inhibit NK cell-mediated lysis (Fuchs et al. 2004).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Human SK-N-S1 neuroblastoma cells.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P15151
- Entrez Gene
- PVR
- GO Terms
- GO:0002860 positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
- GO:0016021 integral to membrane
- GO:0050839 cell adhesion molecule binding
- GO:0005615 extracellular space
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0009986 cell surface
- GO:0030054 cell junction
- GO:0034329 cell junction assembly
- GO:0034332 adherens junction organization
- GO:0042271 susceptibility to natural killer cell mediated cytotoxicity
- GO:0044419 interspecies interaction between organisms
- GO:0060370 susceptibility to T cell mediated cytotoxicity
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD155 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | |||
| Flow Cytometry | 1/25 | 1/200 | |
| Functional Assays | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Flow Cytometry | 1/25 | 1/200 | |
| Immunofluorescence | |||
| Immunohistology - Frozen |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
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| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
|
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| Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | C F | 0.5 mg |
|
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| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
Source Reference
-
Fuchs, A. et al. (2004) Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155).
J Immunol. 172 (7): 3994-8.
References for CD155 antibody
-
Fuchs, A. et al. (2005) Paradoxic inhibition of human natural interferon-producing cells by the activating receptor NKp44.
Blood. 106 (6): 2076-82. -
Boles, K.S. et al. (2009) A novel molecular interaction for the adhesion of follicular CD4 T cells to follicular DC.
Eur J Immunol. 39 (3): 695-703. -
Cella, M. et al. (2010) Loss of DNAM-1 contributes to CD8+ T-cell exhaustion in chronic HIV-1 infection.
Eur J Immunol. 40 (4): 949-54.
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