CD154 antibody | TRAP-1

Mouse anti Human CD154 (CD40L)

Product Type
Monoclonal Antibody
Clone
TRAP-1
Isotype
IgG1
Specificity
CD154

Product Code Applications Pack Size List Price Your Price Qty
MCA1561
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD154 antibody, clone TRAP-1 recognises the human CD40 ligand, also known as CD154, TNF-related activation protein (TRAP) or T-cell antigen Gp39. CD154 is a 261 amino acid ~32 kDa single pass, type-1 transmembrane glycoprotein (UniProt: P29965). CD154 is expressed on activated T lymphocytes, predominantly CD4 +ve and also on some basophils and mast cells.

Mouse anti Human CD154 antibody, clone TRAP-1 binds to CD154 at an epitope distinct from the CD40 binding site (Kroczek et al. 1994).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Mouse myeloma cell line transfected with human CD40L (CD154)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63.Ag 8653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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References for CD154 antibody

  1. Hermann, P. et al. (1993) Expression of a 32-kDa ligand for the CD40 antigen on activated human T lymphocytes.
    Eur J Immunol. 23 (4): 961-4.
  2. Lane, P. et al. (1992) Activated human T cells express a ligand for the human B cell-associated antigen CD40 which participates in T cell-dependent activation of B lymphocytes.
    Eur J Immunol. 22 (10): 2573-8.
  3. Kroczek, R.A et al. (1994) Defective expression of CD40 ligand on T cells causes "X-linked immunodeficiency with hyper-IgM (HIGM1)".
    Immunol Rev. 138: 39-59.
  4. Houtkamp, M.A. et al. (2001) Interleukin-15 expression in atherosclerotic plaques: an alternative pathway for T-cell activation in atherosclerosis?
    Arterioscler Thromb Vasc Biol. 21: 1208-13.
  5. Yarwood, H. et al. (2000) Resting and activated T cells induce expression of E-selectin and VCAM-1 by vascular endothelial cells through a contact-dependent but CD40 ligand-independent mechanism.
    J Leukoc Biol. 68: 233-42.

Synonyms
CD40 Ligand
RRID
AB_321588
UniProt
P29965
Entrez Gene
CD40LG
GO Terms
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005174 CD40 receptor binding
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0007159 leukocyte cell-cell adhesion
GO:0030168 platelet activation
GO:0032735 positive regulation of interleukin-12 production
GO:0042100 B cell proliferation
GO:0045190 isotype switching
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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