CD154 antibody | TRAP-1
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Mouse anti Human CD154 (CD40L)
- Product Type
- Monoclonal Antibody
- Clone
- TRAP-1
- Isotype
- IgG1
- Specificity
- CD154
Antibody Quality and Performance Guarantee
| Mouse anti Human CD154 antibody, clone TRAP-1 recognises the human CD40 ligand, also known as CD154, TNF-related activation protein (TRAP) or T-cell antigen Gp39. CD154 is a 261 amino acid ~32 kDa single pass, type-1 transmembrane glycoprotein (UniProt: P29965). CD154 is expressed on activated T lymphocytes, predominantly CD4 +ve and also on some basophils and mast cells. Mouse anti Human CD154 antibody, clone TRAP-1 binds to CD154 at an epitope distinct from the CD40 binding site (Kroczek et al. 1994). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Mouse myeloma cell line transfected with human CD40L (CD154)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63.Ag 8653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD154 antibody
-
Hermann, P. et al. (1993) Expression of a 32-kDa ligand for the CD40 antigen on activated human T lymphocytes.
Eur J Immunol. 23 (4): 961-4. -
Lane, P. et al. (1992) Activated human T cells express a ligand for the human B cell-associated antigen CD40 which participates in T cell-dependent activation of B lymphocytes.
Eur J Immunol. 22 (10): 2573-8. -
Kroczek, R.A et al. (1994) Defective expression of CD40 ligand on T cells causes "X-linked immunodeficiency with hyper-IgM (HIGM1)".
Immunol Rev. 138: 39-59. -
Houtkamp, M.A. et al. (2001) Interleukin-15 expression in atherosclerotic plaques: an alternative pathway for T-cell activation in atherosclerosis?
Arterioscler Thromb Vasc Biol. 21: 1208-13. -
Yarwood, H. et al. (2000) Resting and activated T cells induce expression of E-selectin and VCAM-1 by vascular endothelial cells through a contact-dependent but CD40 ligand-independent mechanism.
J Leukoc Biol. 68: 233-42.
- Synonyms
- CD40 Ligand
- RRID
- AB_321588
- UniProt
- P29965
- Entrez Gene
- CD40LG
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005174 CD40 receptor binding
- GO:0005615 extracellular space
- GO:0005625 soluble fraction
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0007159 leukocyte cell-cell adhesion
- View More GO Terms
- GO:0030168 platelet activation
- GO:0032735 positive regulation of interleukin-12 production
- GO:0042100 B cell proliferation
- GO:0045190 isotype switching
View more products with CD154 specificity
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