CD147 antibody | MEM-M6/6

100% Secure

Mouse anti Human CD147:Preservative Free

Product Type
Monoclonal Antibody
Clone
MEM-M6/6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2882Z F FN WB 0.1 mg
Mouse anti Human CD147 antibody, clone MEM-M6/6 recognizes the Ig-like V-type domain of human CD147, otherwise known as basigin, neurothelin or extracellular matrix metalloproteinase inducer (EMMPRIN). CD147 is a 364 amino acid, ~50-55 kDa single pass type I transmembrane glycoprotein and member of the immunoglobulin gene superfamily, containing a single Ig-like C2-type and a single Ig-like V-type domain. CD147 is broadly expressed on peripheral blood cells, endothelial cells, and increased on T cells following activation. CD147 is also strongly expressed on small lung cancer cells and hepatocellular carcinoma.

Biological properties of CD147 include the regulation of T cells, integrin functions and cell adhesion, the stimulation of metalloproteinase (MMP) production by fibroblasts, the promotion of astrocyte outgrowths, and roles in tumour progression, neuronal system function and reproduction (Muramatsu et al. 2003).

There are four distinct isoforms of CD147 described in UniProt entry P35613 resulting from alternative splicing or differing promotor usage, the canonical version described here and three alternative truncated isoforms with alterations and deletions to the N-terminal region. The epitope recognized by mouse anti human CD147, clone MEM-M6/6 lies C-terminally to the altered sequences in these isoforms and clone MEM-M6/6 would therefore be expected to bind to all described forms of human CD147.

Mouse anti Human CD147, clone MEM-M6/6 inhibits anti-CD3-induced T cell activation, dependent upon antibody concentration (Koch et al. 1999).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
CD147Rg, consisting of the cDNA coding for the extracellular region of CD147 expressed in CHO cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P35613 Related reagents
Entrez Gene
BSG Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0005537 mannose binding
GO:0006090 pyruvate metabolic process
GO:0007166 cell surface receptor linked signaling pathway
GO:0015129 lactate transmembrane transporter activity
GO:0042470 melanosome
GO:0050900 leukocyte migration
Regulatory
For research purposes only

Applications of CD147 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Functional Assays
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Western Blotting
MCA2882Z detects a band of approximately 45-60kDa in 293 human fibroblastoid cell lysates under non-reducing conditions.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Functional Assays

Product Specific References

References for CD147 antibody

  1. Koch, C. et al. (1999) T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density.
    Int Immunol. 11 (5): 777-86.
  2. Nassif, X. et al. (2015) Cd147 as receptor for pilus-mediated adhesion of meningococci to vascular endothelia.
    Patent Publication number US20150110806 A1
  3. Bernard SC et al. (2014) Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization.
    Nat Med. 20 (7): 725-31.
  4. Douglas, A.D. et al. (2014) Neutralization of Plasmodium falciparum merozoites by antibodies against PfRH5.
    J Immunol. 192 (1): 245-58.
  5. Crosnier C et al. (2011) Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum.
    Nature. 480 (7378): 534-7.
  6. Wanaguru M et al. (2013) RH5-Basigin interaction plays a major role in the host tropism of Plasmodium falciparum.
    Proc Natl Acad Sci U S A. 110 (51): 20735-40.
  7. Zenonos, Z.A. et al. (2015) Basigin is a druggable target for host-oriented antimalarial interventions.
    J Exp Med. 212 (8): 1145-51.
  8. Hahn, J.N. et al. (2015) The role of EMMPRIN in T cell biology and immunological diseases.
    J Leukoc Biol. 98 (1): 33-48.