CD147 antibody | MEM-M6/1
Mouse anti Human CD147 antibody, clone MEM-M6/1 (MCA1876) used for the demonstration of CD147 expression in HBMEC cells by immunofluorescence.
Image caption:
CD147 and the β2AR colocalize at endothelial cell junctions and at sites of bacterial adhesion. HBMECs, transiently transfected with β2AR-YFP, were non-infected (top) or infected with meningococci for 2 h (bottom). CD147 (red) and β2AR-YFP (green) receptor localization was analysed by confocal microscopy (scale bars, 10μm).
From: Maïssa N, Covarelli V, Janel S, Durel B, Simpson N, Bernard SC, Pardo-Lopez L, Bouzinba-Ségard H, Faure C, Scott MGH, Coureuil M, Morand PC, Lafont F, Nassif X, Marullo S, Bourdoulous S.
Strength of Neisseria meningitidis binding to endothelial cells requires highly-ordered CD147/β2-adrenoceptor clusters assembled by alpha-actinin-4.
Nat Commun. 2017 Jun 1;8:15764.
doi: 10.1038/ncomms15764..
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD147 antibody, clone MEM-M6/1 (MCA1876) used for the demonstration of CD147 expression in HBMEC cells by immunoprecipitation and western blotting.
Image caption:
Actn4 co-immunoprecipitates with CD147. HBMECs were either non-infected (Ni) or infected with meningococci for the indicated period of time before lysis. CD147 was immunoprecipitated (irrelevant antibody (Ig) as control) and the obtained samples were successively immunoblotted with anti-Actn4 (top) and anti-CD147 antibodies (bottom). The total cell lysate (TCL) was loaded as a control.
From: Maïssa N, Covarelli V, Janel S, Durel B, Simpson N, Bernard SC, Pardo-Lopez L, Bouzinba-Ségard H, Faure C, Scott MGH, Coureuil M, Morand PC, Lafont F, Nassif X, Marullo S, Bourdoulous S.
Strength of Neisseria meningitidis binding to endothelial cells requires highly-ordered CD147/β2-adrenoceptor clusters assembled by alpha-actinin-4.
Nat Commun. 2017 Jun 1;8:15764.
doi: 10.1038/ncomms15764..
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD147 antibody, clone MEM-M6/1 (MCA1876) used for the demonstration of CD147 expression in HBMEC cells by immunofluorescence.
Image caption:
CD147 and Actn4 colocalize at endothelial cell junctions and at sites of bacterial adhesion. HBMECs non-infected (top) or infected for 2 h with meningococci (bottom) were stained for CD147 (green), Actn4 (red) and actin (blue) and analysed by confocal microscopy. Merged images of the same fields are presented on the right (scale bar, 10 μm).
From: Maïssa N, Covarelli V, Janel S, Durel B, Simpson N, Bernard SC, Pardo-Lopez L, Bouzinba-Ségard H, Faure C, Scott MGH, Coureuil M, Morand PC, Lafont F, Nassif X, Marullo S, Bourdoulous S.
Strength of Neisseria meningitidis binding to endothelial cells requires highly-ordered CD147/β2-adrenoceptor clusters assembled by alpha-actinin-4.
Nat Commun. 2017 Jun 1;8:15764.
doi: 10.1038/ncomms15764..
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD147 antibody, clone MEM-M6/1 (MCA1876) used for the demonstration of CD147 expression in HBMEC cells by immunofluorescence.
Image caption:
Actn4 accumulates at sites of bacterial adhesion independently of PI(4,5)P2 production and cortical actin polymerization. HBMECs were transfected with GFP-tagged PH domain of PLC-δ1, along with the membrane-targeted FRB-CFP and the mRFP-FKBP domain constructs (with or without 5'-phosphatase domain as indicated), infected with meningococci for 2 h in the presence of rapamycin to induce the translocation of 5′-phosphatase to the plasma membrane and stained for ezrin, actin, CD147 and Actn4. The colour bar indicates the pixel intensity of normalized fluorescence images (in arbitrary units). Arrows point at sites of bacterial adhesion. Control GFP, mRFP-FKBP, mRFP-FKBP-5-phosphatase and FRB-CFP fluorescence images are shown in Supplementary Fig. 5. Images are representative of five independent experiments (scale bars, 10 μm).
From: Maïssa N, Covarelli V, Janel S, Durel B, Simpson N, Bernard SC, Pardo-Lopez L, Bouzinba-Ségard H, Faure C, Scott MGH, Coureuil M, Morand PC, Lafont F, Nassif X, Marullo S, Bourdoulous S.
Strength of Neisseria meningitidis binding to endothelial cells requires highly-ordered CD147/β2-adrenoceptor clusters assembled by alpha-actinin-4.
Nat Commun. 2017 Jun 1;8:15764.
doi: 10.1038/ncomms15764..
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD147 antibody, clone MEM-M6/1 (MCA1876) used for the demonstration of CD147 expression in HBMEC cells by immunofluorescence.
Image caption:
(a and b) CD147 organizes in dense clusters surrounding adherent bacteria. (a) HBMECs cells were non-infected or infected for 1 h with meningococci, stained for bacteria and CD147, and analysed by widefield and STORM microscopy on a Leica SR GSD. Fifty thousand frames were recorded for each acquisition and reconstructed using the Leica Application Suite (LAS X) core module. (b) HBMECs cells infected for 1 h with meningococci were stained for CD147 and 4,6-diamidino-2-phenylindole (DAPI) to visualize bacteria and cell nuclei (N); imaging was performed on a iMIC imaging work station. Ten thousand frames were recorded and reconstructed using QuickPALM 1.1 ImageJ plug (scale bar, 5 μm). Bottom: Typical STORM reconstruction sequence; legend indicates the number of acquired images used for reconstruction. (c) Comparative 2D- and 3D-STORM analysis of CD147 distribution at bacterial adhesion sites. HBMECs cells were infected for 1 h with meningococci and stained for CD147. Images were acquired as in (a), on a Leica SR GSD. Shown are the 2D- and 3D-STORM reconstructions of CD147 clusters assembled at bacterial adhesion sites using the Leica LAS X 3D Visualization (scale bars, 1 μm) and the depth colour code (0–800 nm). (d) 3D-SIM analysis of CD147 distribution at bacterial adhesion sites. 3D-SIM reconstruction of CD147 and Actn4 clusters at bacterial adhesion sites (scale bar, 2μm).
From: Maïssa N, Covarelli V, Janel S, Durel B, Simpson N, Bernard SC, Pardo-Lopez L, Bouzinba-Ségard H, Faure C, Scott MGH, Coureuil M, Morand PC, Lafont F, Nassif X, Marullo S, Bourdoulous S.
Strength of Neisseria meningitidis binding to endothelial cells requires highly-ordered CD147/β2-adrenoceptor clusters assembled by alpha-actinin-4.
Nat Commun. 2017 Jun 1;8:15764.
doi: 10.1038/ncomms15764..
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD147:APC
Mouse anti Human CD147:Preservative Free
Mouse anti Human CD147
Mouse anti Human CD147:RPE
- Product Type
- Monoclonal Antibody
- Clone
- MEM-M6/1
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA1876APC | F | 100 Tests |
|
|
| MCA1876XZ | F IP WB* | 1 mg |
|
|
| MCA1876 | F IP WB* | 0.2 mg |
|
|
| MCA1876PE | F | 100 Tests |
|
Mouse anti Human CD147 antibody, clone MEM-M6/1 recognizes the human CD147 cell surface antigen, also known as Basigin, EMMPRIN and collagenase stimulatory factor. CD147 is a 385 amino acid single pass type 1 trans-membrane glycoprotein bearing 3 potential N-glycosylation sites, a single N-terminal (distal) Ig-like C2-type domain amd a more proximal Ig-like V-type domain in its extracellular region. Mouse anti Human CD147 antibody, clone MEM-M6/1 was raised and screened against recombinant extracellular region of huCD147 expressed in CHO cells, along with a number of other MEM-M6 clones. Epitope mapping indicates that clone MEM-M6/1 binds to an epitope within the distal Ig-like C-2 type domain. This is confirmed by flow cytometric studies using beads coated with constructs containing only the distal Ig-like or proximal Ig-like domains (Koch et al. 1999).
Mouse anti Human CD147 antibody, clone MEM-M6/1 binds to both un-stimulated and phytohemagglutinin (PHA) stimulated (activated) T lymphocytes with levels of CD147 enhanced following PHA stimulation. CD147 is also expressed by peripheral blood and endothelial cells as well as many cultured cells of hematopoietic and non-hematopoietic origin (Koch et al. 1999). In addition to clone MEM-M6/1 we are also able to offer the MEM-M6/6 clone derived from the same fusion which recognizes an epitope in the membrane proximal Ig-like V type domain and has inhibitory effects on CD3 induced T cell activation.
Mouse anti Human CD147 antibody, clone MEM-M6/1 binds to both un-stimulated and phytohemagglutinin (PHA) stimulated (activated) T lymphocytes with levels of CD147 enhanced following PHA stimulation. CD147 is also expressed by peripheral blood and endothelial cells as well as many cultured cells of hematopoietic and non-hematopoietic origin (Koch et al. 1999). In addition to clone MEM-M6/1 we are also able to offer the MEM-M6/6 clone derived from the same fusion which recognizes an epitope in the membrane proximal Ig-like V type domain and has inhibitory effects on CD3 induced T cell activation.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by hydrophobic charge induction chromatography.
- Preparation
- Purified IgG prepared by Protein A affinity chromatography.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Immunogen
- CD147Rg, consisting of the cDNA coding for the extracellular region of human CD147.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with SP2/0 mouse myeloma cells.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of reconstitution.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P35613 Related reagents
- Entrez Gene
- BSG Related reagents
- GO Terms
- GO:0000139 Golgi membrane
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0016021 integral to membrane
- GO:0005537 mannose binding
- GO:0006090 pyruvate metabolic process
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0015129 lactate transmembrane transporter activity
- GO:0042470 melanosome
- GO:0050900 leukocyte migration
- Regulatory
- For research purposes only
Applications of CD147 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/10 | 1/50 | |
| Immunoprecipitation | |||
| Western Blotting 1 | |||
| Flow Cytometry | 1/50 | 1/100 | |
| Immunoprecipitation | |||
| Western Blotting 1 | |||
| Flow Cytometry | Neat |
- 1MEM-M6/1 recognizes CD147 under non-reducing conditions.
- 1MEM-M6/1 recognizes CD147 under non-reducing conditions.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:APC | MCA928APC | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD147 antibody
-
Koch, C. et al. (1999) T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density.
Int Immunol. 11 (5): 777-86. -
Yang, Y. et al. (2008) Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis.
Rheumatology (Oxford). 47: 1299-310. -
Schneiderhan, W. et al. (2007) Pancreatic stellate cells are an important source of MMP-2 in human pancreatic cancer and accelerate tumor progression in a murine xenograft model and CAM assay.
J Cell Sci. 120: 512-9. -
Chen, Y. et al. (2009) Upregulation of HAb18G/CD147 in activated human umbilical vein endothelial cells enhances the angiogenesis.
Cancer Lett. 278: 113-21. -
Schneiderhan, W. et al. (2009) CD147 silencing inhibits lactate transport and reduces malignant potential of pancreatic cancer cells in in vivo and in vitro models
Gut. 58: 1391-8. -
Wright, G.J. et al. (2014) Immunogenic compositions and expression systems.
Patent Publication number. US20140093540 A1 -
Huang, Z. et al. (2013) Overexpression of CD147 contributes to the chemoresistance of head and neck squamous cell carcinoma cells.
J Oral Pathol Med. 42: 541-6. -
Hu, J. et al. (2010) Involvement of HAb18G/CD147 in T cell activation and immunological synapse formation.
J Cell Mol Med. 14 (8): 2132-43. -
Bernard, S.C. et al. (2014) Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization.
Nat Med. 20 (7): 725-31. -
Nassif, X. et al. (2015) Cd147 as receptor for pilus-mediated adhesion of meningococci to vascular endothelia.
Patent Publication number US20150110806 A1 -
Trakarnsanga, K. et al. (2017) An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
Nat Commun. 8: 14750. -
Maïssa, N. et al. (2017) Strength of Neisseria meningitidis binding to endothelial cells requires highly-ordered CD147/β2-adrenoceptor clusters assembled by alpha-actinin-4.
Nat Commun. 8: 15764.
