CD147 antibody | MEM-M6/1

Mouse anti Human CD147

Product Type
Monoclonal Antibody
Clone
MEM-M6/1
Isotype
IgG1
Format
Purified
Specificity
CD147

Product Code Applications Pack Size List Price Your Price Qty
MCA1876
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP WB * 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD147 antibody, clone MEM-M6/1 recognizes the human CD147 cell surface antigen, also known as Basigin, EMMPRIN and collagenase stimulatory factor. CD147 is a 385 amino acid single pass type 1 trans-membrane glycoprotein bearing 3 potential N-glycosylation sites, a single N-terminal (distal) Ig-like C2-type domain amd a more proximal Ig-like V-type domain in its extracellular region. Mouse anti Human CD147 antibody, clone MEM-M6/1 was raised and screened against recombinant extracellular region of huCD147 expressed in CHO cells, along with a number of other MEM-M6 clones. Epitope mapping indicates that clone MEM-M6/1 binds to an epitope within the distal Ig-like C-2 type domain. This is confirmed by flow cytometry using beads coated with constructs containing only the distal Ig-like or proximal Ig-like domains (Koch et al. 1999).

Mouse anti Human CD147 antibody, clone MEM-M6/1 binds to both un-stimulated and phytohemagglutinin (PHA) stimulated (activated) T lymphocytes with levels of CD147 enhanced following PHA stimulation. CD147 is also expressed by peripheral blood and endothelial cells as well as many cultured cells of hematopoietic and non-hematopoietic origin (Koch et al. 1999). In addition to clone MEM-M6/1 we are also able to offer the MEM-M6/6 clone derived from the same fusion which recognizes an epitope in the membrane proximal Ig-like V type domain and has inhibitory effects on CD3 induced T cell activation (Kock et al. 1999)
PrecisionAb Antibodies

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by Protein A affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
CD147Rg, consisting of the cDNA coding for the extracellular region of human CD147.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with SP2/0 mouse myeloma cells.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunoprecipitation
Western Blotting 1
  1. 1MEM-M6/1 recognizes CD147 under non-reducing conditions.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (Fc):HRP
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control

References for CD147 antibody

  1. Koch, C. et al. (1999) T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density.
    Int Immunol. 11 (5): 777-86.
  2. Yang, Y. et al. (2008) Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis.
    Rheumatology (Oxford). 47: 1299-310.
  3. Schneiderhan, W. et al. (2007) Pancreatic stellate cells are an important source of MMP-2 in human pancreatic cancer and accelerate tumor progression in a murine xenograft model and CAM assay.
    J Cell Sci. 120: 512-9.
  4. Chen, Y. et al. (2009) Upregulation of HAb18G/CD147 in activated human umbilical vein endothelial cells enhances the angiogenesis.
    Cancer Lett. 278: 113-21.
  5. Schneiderhan, W. et al. (2009) CD147 silencing inhibits lactate transport and reduces malignant potential of pancreatic cancer cells in in vivo and in vitro models
    Gut. 58: 1391-8.
  6. Wright, G.J. et al. (2014) Immunogenic compositions and expression systems.
    Patent Publication number. US20140093540 A1
  7. Huang, Z. et al. (2013) Overexpression of CD147 contributes to the chemoresistance of head and neck squamous cell carcinoma cells.
    J Oral Pathol Med. 42: 541-6.
  8. Hu, J. et al. (2010) Involvement of HAb18G/CD147 in T cell activation and immunological synapse formation.
    J Cell Mol Med. 14 (8): 2132-43.
  9. View The Latest Product References
  10. Bernard, S.C. et al. (2014) Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization.
    Nat Med. 20 (7): 725-31.
  11. Nassif, X. et al. (2015) Cd147 as receptor for pilus-mediated adhesion of meningococci to vascular endothelia.
    Patent Publication number US20150110806 A1
  12. Trakarnsanga, K. et al. (2017) An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
    Nat Commun. 8: 14750.

Flow Cytometry

Synonyms
Neurothelin
RRID
AB_322225
UniProt
P35613
Entrez Gene
BSG
GO Terms
GO:0000139 Golgi membrane
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0005537 mannose binding
GO:0006090 pyruvate metabolic process
GO:0007166 cell surface receptor linked signaling pathway
GO:0015129 lactate transmembrane transporter activity
GO:0042470 melanosome
GO:0050900 leukocyte migration
View more products with CD147 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products