CD138 antibody | rB18-02-5H10
Mouse anti Human CD138
- Product Type
- Monoclonal Antibody
- Clone
- rB18-02-5H10
- Isotype
- IgG1
- Specificity
- CD138
Antibody Quality and Performance Guarantee
TZA0134GA
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| Mouse anti Human CD138 antibody, clone rB18-02-5H10, is a recombinant monoclonal antibody that recognizes CD138, also known as Syndecan-1 or SDC-1. CD138 is a member of the transmembrane heparan sulfate proteoglycan family. It is composed of a core protein covalently attached to heparan sulfate and chondroitin sulfate chains. CD138 is expressed on the surface of plasma cells within the hematopoietic system and on the surface of mature epithelial cells. CD138 acts as an extracellular matrix receptor, involved in many cellular functions, including cell binding, cell signaling and cytoskeletal organization through cell-cell adhesion and cell-matrix adhesion. It can bind to numerous proteins, including integrins and extracellular matrix components. CD138 is also involved in regulating exosome biogenesis, alongside SDCBP and PDCD6IP. The extracellular domain is proteolytically cleaved in a shedding process and deposited in the extracellular matrix where it exerts autocrine and paracrine effects. The CD138 marker is used in flow cytometry to identify normal or malignant plasma cells. CD138 is upregulated in malignant plasma cells and has a causal role in the progression of multiple myeloma (MM). CD138 expression level correlates with MM disease severity and is used an index of the effectiveness of the treatment regimen. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Mammalian cells transfected with full length CD138
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
- UniProt
- P18827
- Entrez Gene
- SDC1
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005737 cytoplasm
- GO:0005925 focal adhesion
- GO:0006629 lipid metabolic process
- GO:0008022 protein C-terminus binding
- GO:0008092 cytoskeletal protein binding
- GO:0042157 lipoprotein metabolic process
- GO:0048627 myoblast development
- GO:0055002 striated muscle cell development
- View More GO Terms
View more products with CD138 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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