CD137 antibody | 4B4-1

Mouse anti Human CD137

Product Type
Monoclonal Antibody
Clone
4B4-1
Isotype
IgG1
Specificity
CD137

Product Code Applications Pack Size List Price Your Price Qty
MCA6117
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F FN IP 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD137, clone 4B4-1 recognizes CD137. CD137 (also known as 4-1BB ligand receptor or TNFRSF9) is a type I transmembrane glycoprotein of the Tumor Necrosis Factor receptor family that is expressed on activated T and NK cells, dendritic cells, myeloid cells and at the surface of some non-lymphoid cells under specific conditions (Schwarz et al. 1995). CD137 acts as a receptor for 4-1BB ligand, expressed on activated macrophages, activated B cells, hematopoietic stem cells and myeloid progenitor cells (Pollock et al. 1994) and for some proteins of the extracellular matrix (Chalupny et al. 1992).

The interaction between CD137 and 4-1BBL acts as a co-stimulatory signal for the activation, expansion and survival of T cells (Wen et al. 2002). CD137 signaling is involved in the generation of CD8+ T cells and enhance their cytotoxic activity (Shuford et al. 1997). Because of its ability to promote the anti-tumorigenic activity of cytotoxic T cells, CD137 is the target of several cancer immune therapies, such as the agonist anti-CD137 Urelumab (Segal et al. 2017) and Utomilumab (Chester et al. 2018).
CD137 also binds to the adaptor proteins TRAF1, 2 and 3 to promote NF-kappaB activation (Jang et al. 1998).

Mouse anti-human CD137, clone 4B4-1 has been reported to immunoprecipitate a homodimeric protein of 32kDa under reducing conditions and 85kDa under non-reducing conditions (Garni-Wagner et al. 1996).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Recombinant human CD137 ectodomain
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Functional Assays
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for CD137 antibody

  1. Schwarz, H. et al. (1995) ILA, the human 4-1BB homologue, is inducible in lymphoid and other cell lineages.
    Blood. 85 (4): 1043-52.
  2. Pollok, K.E. et al. (1994) 4-1BB T-cell antigen binds to mature B cells and macrophages, and costimulates anti-mu-primed splenic B cells.
    Eur J Immunol. 24 (2): 367-74.
  3. Chalupny, N.J. et al. (1992) T-cell activation molecule 4-1BB binds to extracellular matrix proteins.
    Proc Natl Acad Sci U S A. 89 (21): 10360-4.
  4. Wen, T. et al. (2002) 4-1BB ligand-mediated costimulation of human T cells induces CD4 and CD8 T cell expansion, cytokine production, and the development of cytolytic effector function.
    J Immunol. 168 (10): 4897-906.
  5. Shuford, W.W. et al. (1997) 4-1BB costimulatory signals preferentially induce CD8+ T cell proliferation and lead to the amplification in vivo of cytotoxic T cell responses.
    J Exp Med. 186 (1): 47-55.
  6. Segal, N.H. et al. (2017) Results from an Integrated Safety Analysis of Urelumab, an Agonist Anti-CD137 Monoclonal Antibody.
    Clin Cancer Res. 23 (8): 1929-1936.
  7. Chester, C. et al. (2018) Immunotherapy targeting 4-1BB: mechanistic rationale, clinical results, and future strategies.
    Blood. 131 (1): 49-57.
  8. Jang, I.K. et al. (1998) Human 4-1BB (CD137) signals are mediated by TRAF2 and activate nuclear factor-kappa B.
    Biochem Biophys Res Commun. 242 (3): 613-20.
  9. View The Latest Product References
  10. Garni-wagner, B.A. et al. (1996) 4-1BB is expressed on CD45RAhiROhi transitional T cell in humans.
    Cell Immunol. 169 (1): 91-8.

Synonyms
TNFRSF9
UniProt
Q07011
Entrez Gene
TNFRSF9
GO Terms
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0006917 induction of apoptosis
GO:0008285 negative regulation of cell proliferation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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