Mouse anti Human CD130 antibody, clone B-T2
recognizes soluble and membrane bound gp130 receptor, also known as CD130, Interleukin-6 receptor subunit beta, Oncostatin-M receptor subunit alpha or Interleukin-6 signal transducer. CD130 is a 918 amino acid, ~130 kDa single pass type-1 transmembrane glycoprotein containing a single Ig-like C2 type
and multiple fibronectin type-III
domains. CD130 can be cleaved to form a monomeric soluble ~100 kDa form of the receptor detectable in plasma and other biologic fluids where it acts as an IL-6 antagonist (Müller-Newen et al. 1998
). Mouse anti Human CD130 antibody, clone B-T2 binds to an epitope dependent at least partially on the presence of the Ig-like domain (Hammacher et al. 1998
Mouse anti Human CD130 antibody, clone B-T2 has been reported to block receptor activity and inhibit IL-6 induced proliferation of XG1 cells and IL-27 mediated proliferation of naive CD4+
T cells(de Groot et al. 2012
, Pflanz et al. 2004
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Natural soluble gp130 receptor.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c (Iffa Credo) mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
positive regulation of acute inflammatory response
positive regulation of adaptive immune response
ciliary neurotrophic factor receptor activity
ciliary neurotrophic factor receptor binding
interleukin-6 receptor complex
oncostatin-M receptor complex
positive regulation vascular endothelial growth factor production
positive regulation of cardiac muscle hypertrophy
response to cytokine stimulus
positive regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
protein homodimerization activity
interleukin-27 receptor activity
positive regulation of osteoblast differentiation
positive regulation of anti-apoptosis
leukemia inhibitory factor signaling pathway
negative regulation of interleukin-6-mediated signaling pathway
interleukin-27-mediated signaling pathway
ciliary neurotrophic factor receptor complex
ciliary neurotrophic factor-mediated signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD130 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD130 antibody
Wijdenes, J. et al. (1995) Interleukin-6 signal transducer gp130 has specific binding sites for different cytokines as determined by antagonistic and agonistic anti-gp130 monoclonal antibodies.
Eur J Immunol. 25 (12): 3474-81.
Cork, B.A. (2002) Expression of interleukin (IL)-11 receptor by the human endometrium in vivo and effects of IL-11, IL-6 and LIF on the production of MMP and cytokines by human endometrial cells in vitro.
Mol Hum Reprod. 8: 841-8.
Zhang, L. et al. (2012) Role of the microenvironment in mantle cell lymphoma: IL-6 is an important survival factor for the tumor cells.
Blood. 120 (18): 3783-92.