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CD130 antibody | B-T2

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Mouse anti Human CD130

Product Type
Monoclonal Antibody
Clone
B-T2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1136
Datasheet
E F
SDS
0.1 mg loader

Mouse anti Human CD130 antibody, clone B-T2 recognizes soluble and membrane bound gp130 receptor, also known as CD130, Interleukin-6 receptor subunit beta, Oncostatin-M receptor subunit alpha or Interleukin-6 signal transducer. CD130 is a 918 amino acid, ~130 kDa single pass type-1 transmembrane glycoprotein containing a single Ig-like C2 type and multiple fibronectin type-III domains. CD130 can be cleaved to form a monomeric soluble ~100 kDa form of the receptor detectable in plasma and other biologic fluids where it acts as an IL-6 antagonist (Müller-Newen et al. 1998). Mouse anti Human CD130 antibody, clone B-T2 binds to an epitope dependent at least partially on the presence of the Ig-like domain (Hammacher et al. 1998).

Mouse anti Human CD130 antibody, clone B-T2 has been reported to block receptor activity and inhibit IL-6 induced proliferation of XG1 cells and IL-27 mediated proliferation of naive CD4+ T cells(de Groot et al. 2012, Pflanz et al. 2004).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Natural soluble gp130 receptor.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c (Iffa Credo) mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P40189
Entrez Gene
IL6ST
GO Terms
GO:0002675 positive regulation of acute inflammatory response
GO:0002821 positive regulation of adaptive immune response
GO:0005576 extracellular region
GO:0004897 ciliary neurotrophic factor receptor activity
GO:0005127 ciliary neurotrophic factor receptor binding
GO:0005615 extracellular space
GO:0005896 interleukin-6 receptor complex
GO:0005900 oncostatin-M receptor complex
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010613 positive regulation of cardiac muscle hypertrophy
GO:0034097 response to cytokine stimulus
GO:0042102 positive regulation of T cell proliferation
GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
GO:0042803 protein homodimerization activity
GO:0045509 interleukin-27 receptor activity
GO:0045669 positive regulation of osteoblast differentiation
GO:0045768 positive regulation of anti-apoptosis
GO:0048861 leukemia inhibitory factor signaling pathway
GO:0070104 negative regulation of interleukin-6-mediated signaling pathway
GO:0070106 interleukin-27-mediated signaling pathway
GO:0070110 ciliary neurotrophic factor receptor complex
GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
Regulatory
For research purposes only

Applications of CD130 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 0.2ug/106cells
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Product Specific References

References for CD130 antibody

  1. Wijdenes, J. et al. (1995) Interleukin-6 signal transducer gp130 has specific binding sites for different cytokines as determined by antagonistic and agonistic anti-gp130 monoclonal antibodies.
    Eur J Immunol. 25 (12): 3474-81.
  2. Cork, B.A. (2002) Expression of interleukin (IL)-11 receptor by the human endometrium in vivo and effects of IL-11, IL-6 and LIF on the production of MMP and cytokines by human endometrial cells in vitro.
    Mol Hum Reprod. 8: 841-8.
  3. Zhang, L. et al. (2012) Role of the microenvironment in mantle cell lymphoma: IL-6 is an important survival factor for the tumor cells.
    Blood. 120 (18): 3783-92.

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