CD130 antibody | B-T2

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Mouse anti Human CD130

Product Type: Monoclonal Antibody
Clone: B-T2
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1136	E F			0.1 mg

Mouse anti Human CD130 antibody, clone B-T2 recognizes soluble and membrane bound gp130 receptor, also known as CD130, Interleukin-6 receptor subunit beta, Oncostatin-M receptor subunit alpha or Interleukin-6 signal transducer. CD130 is a 918 amino acid, ~130 kDa single pass type-1 transmembrane glycoprotein containing a single Ig-like C2 type and multiple fibronectin type-III domains. CD130 can be cleaved to form a monomeric soluble ~100 kDa form of the receptor detectable in plasma and other biologic fluids where it acts as an IL-6 antagonist (Müller-Newen et al. 1998). Mouse anti Human CD130 antibody, clone B-T2 binds to an epitope dependent at least partially on the presence of the Ig-like domain (Hammacher et al. 1998).

Mouse anti Human CD130 antibody, clone B-T2 has been reported to block receptor activity and inhibit IL-6 induced proliferation of XG1 cells and IL-27 mediated proliferation of naive CD4⁺ T cells(de Groot et al. 2012, Pflanz et al. 2004).

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by ion exchange chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Natural soluble gp130 receptor.

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c (Iffa Credo) mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P40189 Related reagents
Entrez Gene: IL6ST Related reagents
GO Terms: GO:0002675 positive regulation of acute inflammatory response; GO:0002821 positive regulation of adaptive immune response; GO:0005576 extracellular region; GO:0004897 ciliary neurotrophic factor receptor activity; GO:0005127 ciliary neurotrophic factor receptor binding; GO:0005615 extracellular space; GO:0005896 interleukin-6 receptor complex; GO:0005900 oncostatin-M receptor complex; GO:0010575 positive regulation vascular endothelial growth factor production; GO:0010613 positive regulation of cardiac muscle hypertrophy; GO:0034097 response to cytokine stimulus; GO:0042102 positive regulation of T cell proliferation; GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein; GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein; GO:0042803 protein homodimerization activity; GO:0045509 interleukin-27 receptor activity; GO:0045669 positive regulation of osteoblast differentiation; GO:0045768 positive regulation of anti-apoptosis; GO:0048861 leukemia inhibitory factor signaling pathway; GO:0070104 negative regulation of interleukin-6-mediated signaling pathway; GO:0070106 interleukin-27-mediated signaling pathway; GO:0070110 ciliary neurotrophic factor receptor complex; GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
Regulatory: For research purposes only

Applications of CD130 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry			0.2ug/10⁶cells

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Product Specific References

References for CD130 antibody

Wijdenes, J. et al. (1995) Interleukin-6 signal transducer gp130 has specific binding sites for different cytokines as determined by antagonistic and agonistic anti-gp130 monoclonal antibodies.
Eur J Immunol. 25 (12): 3474-81.
Cork, B.A. (2002) Expression of interleukin (IL)-11 receptor by the human endometrium in vivo and effects of IL-11, IL-6 and LIF on the production of MMP and cytokines by human endometrial cells in vitro.
Mol Hum Reprod. 8: 841-8.
Zhang, L. et al. (2012) Role of the microenvironment in mantle cell lymphoma: IL-6 is an important survival factor for the tumor cells.
Blood. 120 (18): 3783-92.

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