CD130 antibody | B-T2
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Mouse anti Human CD130
- Product Type
- Monoclonal Antibody
- Clone
- B-T2
- Isotype
- IgG1
- Specificity
- CD130
Antibody Quality and Performance Guarantee
| Mouse anti Human CD130 antibody, clone B-T2 recognizes soluble and membrane bound gp130 receptor, also known as CD130, Interleukin-6 receptor subunit beta, Oncostatin-M receptor subunit alpha or Interleukin-6 signal transducer. CD130 is a 918 amino acid, ~130 kDa single pass type-1 transmembrane glycoprotein containing a single Ig-like C2 type
and multiple fibronectin type-III domains. CD130 can be cleaved to form a monomeric soluble ~100 kDa form of the receptor detectable in plasma and other biologic fluids where it acts as an IL-6 antagonist (Müller-Newen et al. 1998). Mouse anti Human CD130 antibody, clone B-T2 binds to an epitope dependent at least partially on the presence of the Ig-like domain (Hammacher et al. 1998). Mouse anti Human CD130 antibody, clone B-T2 has been reported to block receptor activity and inhibit IL-6 induced proliferation of XG1 cells and IL-27 mediated proliferation of naive CD4+ T cells(de Groot et al. 2012, Pflanz et al. 2004). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Natural soluble gp130 receptor.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c (Iffa Credo) mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
0.2ug/106cells |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Wijdenes, J. et al. (1995) Interleukin-6 signal transducer gp130 has specific binding sites for different cytokines as determined by antagonistic and agonistic anti-gp130 monoclonal antibodies.
Eur J Immunol. 25 (12): 3474-81.
References for CD130 antibody
-
Cork, B.A.
(2002) Expression of interleukin (IL)-11 receptor by the human endometrium in vivo and effects of IL-11, IL-6 and LIF on the production of MMP and cytokines by human endometrial cells in vitro.
Mol Hum Reprod. 8: 841-8. -
Zhang, L. et al. (2012) Role of the microenvironment in mantle cell lymphoma: IL-6 is an important survival factor for the tumor cells.
Blood. 120 (18): 3783-92.
- Synonyms
- gp130-R
- RRID
- AB_2125973
- UniProt
- P40189
- Entrez Gene
- IL6ST
- GO Terms
- GO:0002675 positive regulation of acute inflammatory response
- GO:0002821 positive regulation of adaptive immune response
- GO:0005576 extracellular region
- GO:0004897 ciliary neurotrophic factor receptor activity
- GO:0005127 ciliary neurotrophic factor receptor binding
- GO:0005615 extracellular space
- GO:0005896 interleukin-6 receptor complex
- GO:0005900 oncostatin-M receptor complex
- GO:0010575 positive regulation vascular endothelial growth factor production
- View More GO Terms
- GO:0010613 positive regulation of cardiac muscle hypertrophy
- GO:0034097 response to cytokine stimulus
- GO:0042102 positive regulation of T cell proliferation
- GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
- GO:0042517 positive regulation of tyrosine phosphorylation of Stat3 protein
- GO:0042803 protein homodimerization activity
- GO:0045509 interleukin-27 receptor activity
- GO:0045669 positive regulation of osteoblast differentiation
- GO:0045768 positive regulation of anti-apoptosis
- GO:0048861 leukemia inhibitory factor signaling pathway
- GO:0070104 negative regulation of interleukin-6-mediated signaling pathway
- GO:0070106 interleukin-27-mediated signaling pathway
- GO:0070110 ciliary neurotrophic factor receptor complex
- GO:0070120 ciliary neurotrophic factor-mediated signaling pathway
MCA1136
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160863
160868
166748
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