CD120a antibody | H398
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Mouse anti Human CD120a
- Product Type
- Monoclonal Antibody
- Clone
- H398
- Isotype
- IgG2a
- Specificity
- CD120a
Antibody Quality and Performance Guarantee
| Mouse anti Human CD120a antibody, clone H398 recognizes an extracellular domain of the ~55 kDa human TNF receptor (p55, TNF-R1, CD120a). No binding occurs to the ~75 kDa TNF receptor (CD120b). CD120a is weakly expressed by monocytes and granulocytes. Mouse anti Human CD120a antibody, clone H398 may be used to detect high levels of TNFR1, in western blotting under reducing conditions, such as recombinant material, but it is not suitable for detection of TNFR1 in normal cells in this application. Mouse anti Human CD120a antibody, clone H398 inhibits the biological activity of both natural and recombinant human TNFalpha and TNFbeta (Thoma et al. 1990 and Dri et al. 1999). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Purified human tumour necrosis factor receptor type 1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Radioimmunoassays | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Source Reference
-
Thoma, B. et al. (1990) Identification of a 60-kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses.
J Exp Med. 172 (4): 1019-23.
References for CD120a antibody
-
Menegazzi, R. et al. (1994) Evidence that tumor necrosis factor alpha (TNF)-induced activation of neutrophil respiratory burst on biologic surfaces is mediated by the p55 TNF receptor.
Blood. 84 (1): 287-93. -
Weigert, N. et al. (1996) Gastrin secretion from primary cultures of rabbit antral G cells: stimulation by inflammatory cytokines.
Gastroenterology. 110 (1): 147-54. -
Dri, P. et al. (1999) Role of the 75-kDa TNF receptor in TNF-induced activation of neutrophil respiratory burst.
J Immunol. 162 (1): 460-6. -
Kohrgruber, N. et al. (1999) Survival, maturation, and function of CD11c- and CD11c+ peripheral blood dendritic cells are differentially regulated by cytokines.
J Immunol. 163 (6): 3250-9. -
Schett, G. et al. (2003) TNFalpha mediates susceptibility to heat-induced apoptosis by protein phosphatase-mediated inhibition of the HSF1/hsp70 stress response.
Cell Death Differ. 10: 1126-36. -
Kennedy, G. et al. (2010) Biochemical and vascular aspects of pediatric chronic fatigue syndrome.
Arch Pediatr Adolesc Med. 164 (9): 817-23. -
Thiery, J. et al. (2003) Potentiation of a tumor cell susceptibility to autologous CTL killing by restoration of wild-type p53 function.
J Immunol. 170: 5919-26. -
Gregory, A.P. et al. (2012) TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis.
Nature. 488 (7412): 508-11.
View The Latest Product References
-
Buckley, C.D. et al. (2005) Identification of a phenotypically and functionally distinct population of long-lived neutrophils in a model of reverse endothelial migration.
J Leukoc Biol.79: 303-11. -
Lin, K.H. et al. (2009) Mechanisms of resveratrol-induced platelet apoptosis.
Cardiovasc Res. 83: 575-85. -
Baker, P.K. et al. (2012) Response of hairy cells to IFN-alpha involves induction of apoptosis through autocrine TNF-alpha and protection by adhesion.
Blood. 100: 647-53. -
Nakamura-Lopez, Y. et al. (2015) RSV P-protein impairs extrinsic apoptosis pathway in a macrophage-like cell line persistently infected with respiratory syncytial virus.
Virus Res. 204: 82-7. -
Yang, F. et al. (2013) Improved retinal ganglion cell survival through retinal microglia suppression by a chinese herb extract, triptolide, in the DBA/2J mouse model of glaucoma.
Ocul Immunol Inflamm. 21 (5): 378-89.
- Synonyms
- TNF-R1
- RRID
- AB_321564
- UniProt
- P19438
- Entrez Gene
- TNFRSF1A
- GO Terms
- GO:0006915 apoptosis
- GO:0005576 extracellular region
- GO:0005031 tumor necrosis factor receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006693 prostaglandin metabolic process
- GO:0006954 inflammatory response
- GO:0008624 induction of apoptosis by extracellular signals
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0044419 interspecies interaction between organisms
- View More GO Terms
- GO:0045121 membrane raft
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0050729 positive regulation of inflammatory response
MCA1340T
153297
160120
MCA1340
153156
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