CD120a antibody | H398

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Mouse anti Human CD120a:Alexa Fluor® 647

Mouse anti Human CD120a:Preservative Free

Mouse anti Human CD120a:FITC

Mouse anti Human CD120a

Mouse anti Human CD120a:RPE

Product Type
Monoclonal Antibody
Clone
H398
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1340A647T F 25 Tests/0.25ml
MCA1340A647 F 100 Tests/1ml
MCA1340XZ E F FN R 1 mg
MCA1340FT F 25 µg
MCA1340F F 0.1 mg
MCA1340T E F R 25 µg
MCA1340 E F R 0.2 mg
MCA1340PET F 25 Tests
MCA1340PE F 100 Tests
Mouse anti Human CD120a antibody, clone H398 recognizes an extracellular domain of the ~55 kDa human TNF receptor (p55, TNF-R1, CD120a). No binding occurs to the ~75 kDa TNF receptor (CD120b). CD120a is weakly expressed by monocytes and granulocytes.

Mouse anti Human CD120a antibody, clone H398 may be used to detect high levels of TNFR1, in western blotting under reducing conditions, such as recombinant material, but it is not suitable for detection of TNFR1 in normal cells in this application.

Mouse anti Human CD120a antibody, clone H398 inhibits the biological activity of both natural and recombinant human TNFalpha and TNFbeta (Thoma et al. 1990 and Dri et al. 1999).

Mouse anti Human CD120a antibody, clone H398 is routinely tested in flow cytometry on human peripheral blood monocytes.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647- liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 25 Tests
Reconstitute with 0.25 ml distilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
Pack Size: 25 Tests
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Pack Size: 100 Tests
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Purified human tumour necrosis factor receptor type 1.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 25 Tests
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P19438 Related reagents
Entrez Gene
TNFRSF1A Related reagents
GO Terms
GO:0006915 apoptosis
GO:0005576 extracellular region
GO:0005031 tumor necrosis factor receptor activity
GO:0005887 integral to plasma membrane
GO:0006693 prostaglandin metabolic process
GO:0006954 inflammatory response
GO:0008624 induction of apoptosis by extracellular signals
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0044419 interspecies interaction between organisms
GO:0045121 membrane raft
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050729 positive regulation of inflammatory response
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD120a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
ELISA
Flow Cytometry
Functional Assays
Radioimmunoassays
Flow Cytometry Neat
ELISA
Flow Cytometry
Radioimmunoassays
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA929A647 100 Tests/1ml F
Mouse IgG2a Negative Control:FITC MCA929F 100 Tests F
Mouse IgG2a Negative Control MCA929 100 Tests F
Mouse IgG2a Negative Control:RPE MCA929PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Functional Assays

Product Specific References

References for CD120a antibody

  1. Thoma, B. et al. (1990) Identification of a 60-kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses.
    J Exp Med. 172 (4): 1019-23.
  2. Menegazzi, R. et al. (1994) Evidence that tumor necrosis factor alpha (TNF)-induced activation of neutrophil respiratory burst on biologic surfaces is mediated by the p55 TNF receptor.
    Blood. 84 (1): 287-93.
  3. Dri, P. et al. (1999) Role of the 75-kDa TNF receptor in TNF-induced activation of neutrophil respiratory burst.
    J Immunol. 162 (1): 460-6.
  4. Kohrgruber, N. et al. (1999) Survival, maturation, and function of CD11c- and CD11c+ peripheral blood dendritic cells are differentially regulated by cytokines.
    J Immunol. 163 (6): 3250-9.
  5. Weigert, N. et al. (1996) Gastrin secretion from primary cultures of rabbit antral G cells: stimulation by inflammatory cytokines.
    Gastroenterology. 110 (1): 147-54.
  6. Kennedy, G. et al. (2010) Biochemical and vascular aspects of pediatric chronic fatigue syndrome.
    Arch Pediatr Adolesc Med. 164 (9): 817-23.
  7. Gregory, A.P. et al. (2012) TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis.
    Nature. 488 (7412): 508-11.
  8. Schett, G. et al. (2003) TNFalpha mediates susceptibility to heat-induced apoptosis by protein phosphatase-mediated inhibition of the HSF1/hsp70 stress response.
    Cell Death Differ. 10: 1126-36.
  9. Buckley, C.D. et al. (2005) Identification of a phenotypically and functionally distinct population of long-lived neutrophils in a model of reverse endothelial migration.
    J Leukoc Biol.79: 303-11.
  10. Thiery, J. et al. (2003) Potentiation of a tumor cell susceptibility to autologous CTL killing by restoration of wild-type p53 function.
    J Immunol. 170: 5919-26.
  11. Lin, K.H. et al. (2009) Mechanisms of resveratrol-induced platelet apoptosis.
    Cardiovasc Res. 83: 575-85.
  12. Baker, P.K. et al. (2012) Response of hairy cells to IFN-alpha involves induction of apoptosis through autocrine TNF-alpha and protection by adhesion.
    Blood. 100: 647-53.
  13. Nakamura-Lopez, Y. et al. (2015) RSV P-protein impairs extrinsic apoptosis pathway in a macrophage-like cell line persistently infected with respiratory syncytial virus.
    Virus Res. 204: 82-7.