CD117 antibody | rA21B21-03/4H3-2
Mouse anti Human CD117
- Product Type
- Monoclonal Antibody
- Clone
- rA21B21-03/4H3-2
- Isotype
- IgG1
- Specificity
- CD117
Antibody Quality and Performance Guarantee
TZA0138GA
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| Mouse anti Human CD117 antibody, clone rA21B21-03/4H3-2, is a recombinant monoclonal antibody that recognizes CD117, also known as cKIT and mast/stem cell growth factor receptor (SCFR). CD117 is a member of the type III tyrosine kinase receptor family. It is expressed on hematopoietic stem cells (approx. 70% of CD34+ cells in normal bone marrow are CD117+), germ cells and early hematopoietic progenitors. While expression is lost in some cases during cellular differentiation, it is maintained on mature melanocytes and mast cells. CD117 acts as a cell-surface receptor for stem cell factor (SCF), in response to which it can activate several signalling pathways involved in regulating cell differentiation, chemotaxis, apoptosis, cell adhesion and proliferation. The downstream pathways regulated by CD117 include the JAK/STAT, RAS/MAP kinase, PI3 kinase, and SRC signaling pathways. Antibodies against CD117 are often used as part of a panel for immunophenotyping acute myeloid leukemia (AML). CD117 is expressed in many aggressive cancers and is an indicator of poor survival in patients. In non-small cell lung cancer (NSCLC) it has been highlighted as a potential marker for predicting the prognosis of patients, with CD117‑positive cell populations linked to a shorter relapse‑free survival rate. In mastocytosis, up to 80% of malignant cells are found to have a mutation in CD117. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant CD117 Gln26-Pro524 expressed by mammalian cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
- UniProt
- P10721
- Entrez Gene
- KIT
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0004716 receptor signaling protein tyrosine kinase activity
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005524 ATP binding
- GO:0005615 extracellular space
- GO:0008584 male gonad development
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